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OBJECTIVE: To explore the reversal effect and molecular mechanism of shengqi fuzheng injection(SQFZ) on pancreatic cancer multidrug resistance(MDR) cells. METHODS: Patu8988 was exposured to fluorouracil(5-Fu) to obtain stable 5-Fu-resistant Patu8988/5-Fu cell lines. MTT assay was used to detect the inhibition of SQFZ on cell proliferation and the resistance index of Patu8988/5-Fu and reversal index of SQFZ were calculated. Flow cytometry(FCM) was employed to measure the accumulation of Rho123 in cells. The mRNA expression of MDR1, Bcl-2 and Bax and presence of Bcl-2, Bax and P-gp protein were analyzed by quantitative real-time PCR and Western blot methods respectively. RESULTS: SQFZ at the concentration of 5, 10, 20 μL·mL-1 obviously reversed MDR of Patu8988/5-Fu cells, with the reversal index of 1.5, 2.3 and 3.4 respectively. It increased Rho123 accumulation in a dose-dependent manner in MDR cells(r=0.979, P<0.05). The study revealed that SQFZ downregulated the expressions of MDR1 and Bcl2 gene and upregulated the expression of Bax gene. Furthermore, SQFZ decreased the expression of P-gp and Bcl-2 protein, and increased the expression of Bax protein in Patu8988/5-Fu cells. CONCLUSION: SQFZ can reverse multidrug resistance of Patu8988/FU cells, likely by modulating gene transcription and protein expression of MDRl, Bcl-2 and Bax.
RESUMEN
Objective To explore the effect of SFI in radiation-induced mice brain injury after 20 Gy cranial radiation.Methods The mice were divided into three groups:(1) control group,(2) RT-only group:the whole brain was irradiated with a dose of 20 Gy,(3) RT and SFI group:SFI at 20 ml/kg/d from 4 weeks after 20 Gy cranial radiation theraty(CRT).Results Morris water maze test showed that the latency of the irradiated group was longer than control group and SFI improved the cognitive function of mice (t =6.34,6.70,P ＜0.05).The expression of TNF-α reached to the highest level at 3 h after irradiation,and then it decreased but got the second higher level again at 4 weeks after irradiation.The expression of IL-1 β reached to the highest level at 72 h after irradiation and decreased until 4 weeks after irradiation.SFI decreased both expressions of TNF-α (t =11.34,9.70,6.07,P ＜ 0.05) and IL-1 β (t =12.27,5.70,7.52,P ＜ 0.05).Immune florescence staining showed that SFI reduced the number of activated microglia (t =12.35,8.64,7.82,P ＜ 0.05)and inhibited the translocation of p65 of microglia after irradiation.Conclusions Findings suggest that SFI may decrease microglial activation and suppress the expression of TNF-α and IL-1β by inhibiting the translocation of NF-κB p65 and then attenuate irradiation-induced brain injury.