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AIM: To investigate the expression and effects of sialyl Lewis X (SLeX) on the invasion and migration of human hepatocellular carcinoma HepG2 cells.METHODS: The expression of α1,3-fucosyltransferase VII (FUT7) in HepG2 cells and L-02 cells was detected by RT-qPCR and Western blot.The SLeX expression in HepG2 cells and L-02 cells was determined by Western blot and immunocytochemical staining.The invasion and migration abilities of the treated cells were evaluated by Transwell assay.RESULTS: The expression of FUT7 and SLeX in the HepG2 cells, but not in the L-02 cells, was observed.The invasion rates of the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L were significantly decreased as compared with control group (P<0.05).The migration ability of the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L was also significantly reduced as compared with control group (P<0.05).The invasion rate and migratory cell number were significantly different between any 2 groups in the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L (P<0.05).CONCLUSION: HepG2 cells express SLeX.SLeX is closely related to the migration and invasion abilities of the HepG2 cells.
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The sialyl Lewis X (SLex) antigen encoded by the FUT7 gene is the ligand of endotheliam-selectin (E-selectin).The combination of SLex antigen and E-selectin represents an important way for malignant tumor metastasis.In the present study,the effect of the SLeX-binding DNA aptamer on the adhesion and metastasis of hepatocellular carcinoma HepG2 cells in vitro was investigated.Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence staining were conducted to detect the expression of FUT7 at both transcriptional and translational levels.The SLex expression in HepG2 cells treated with different concentrations of SLeX-binding DNA aptamer was detected by flow cytometry.Besides,the adhesion,migration,and invasion of HepG2 cells were measured by cell adhesion assay,and the Transwell migration and invasion assay.The results showed that the FUT7 expression was up-regulated at both mRNA and protein levels in HepG2 cells.SLeX-binding DNA aptamer could significantly decrease the expression of SLex in HepG2 cells.The cell adhesion assay revealed that the SLeX-binding DNA aptamer could effectively inhibit the interactions between E-selectin and SLex in the HepG2 cells.Additionally,SLeX-binding DNA aptamers at 20 nmol/L were found to have the similar effect to the monoclonal antibody CSLEX-1.The Transwell migration and invasion assay revealed that the number of penetrating cells on the down-side of Transwell membrane was significantly less in cells treated with 5,10,20 nmol/L SLeX-binding DNA aptamer than those in the negative control group (P＜0.01).Our study demonstrated that the SLeX-binding DNA aptamer could significantly inhibit the in vitro adhesion,migration,and invasion of HepG2 cells,suggesting that the SLeX-binding DNA aptamer may be used as a potential molecular targeted drug against metastatic hepatocellular carcinoma.
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Objective To investigate the expressions of matrix metalloproteinase-26(MMP-26) and sialyl Lewis-X(SLeX) in breast carcinoma and their relation with lymphaden metastasis.Methods The expressions of MMP-26 and SLeX protein in breast carcinoma(n=120),metastatic lymph node(n=71) and normal mammary gland tissue(n=30) were studied by catalyzed signal amplification(CSA) immunohistochemical method.Results The positive rate of MMP-26 and SLeX protein expression were 16.7%(5/30) and 20.0%(6/30) in normal breast tissues,and 63.3%(76/120) and 86.7%(104/120) in breast carcinoma,resepectively.The expressions of MMP-26 and SLeX protein in breast carcinoma tissues were significantly higher than those in remote normal breast tissues(P0.05).The positive rate of MMP-26 and SLeX protein expression were 23.9%(17/71) and 88.7%(63/71) in metastatic lymph node,resepectively.The expression of SLeX protein in metastatic lymph node was correlated with that in tissues of breast carcinoma(r=0.874,P
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This study was conducted to study the expressions of sialyl Lewis X and P16 protein in colorectal carcinoma and their relationship with differentiation, metastasis and prognosis of colo rectal carcinoma. Sialyl Lewis X and P16 expressions were determined with microwave-LSAB immunohistochemical technique. The positive rates of sialyl Lewis X and P16 were 92.2% and 42.2% respectively. The increased expression of sialyl Lewis X and the weakened expression of P16 were significantly correlated with metastatic proclivity and poor prognosis of the malignancy. These findings indicated that combined examination of the expression of sialyl Lewis X and P16 might be useful in estimating the differentiation, infitrtion, metastasis of colo-rectal carcinoma and prognosis of the patients.\;