RESUMEN
This research aims to determine the cytotoxicity and antiproliferation activities of Sida rhombifolia leavesextract against cancer cells MCA-B1, A549, and normal Vero cells. Sida rhombifolia leaves were extractedwith ethanol using ultrasonication method and fractionated using n-hexane, ethyl acetate, and water. The testedsamples were ethanol extract and n-hexane fraction based on the results of cytotoxicity using the Brine ShrimpLethality Test. The antiproliferation activity test by using Trypan Blue Dye method and the cells harvested afterconfluence on the third or fourth day and the total cells were calculated by using the Neubauer Hemocytometer.The result showed that the inhibitory activity of ethanol extract at a concentration of 500 ppm is 69.44% withIC50 202.556 ppm on MCA-B1 cancer cells and 69.44% with IC50 276.836 ppm on A549 cancer cells, whilethe n-hexane fraction at a concentration of 1,000 ppm was 64.13% with IC50 425.969 ppm in MCA-B1 cancercells and 57.18% with IC50 786.617 ppm on A549 cancer cells. After being tested on normal Vero cells, theinhibition of normal Vero cells proliferation is not more than 1%. This indicates that ethanol extracts andn-hexane fraction are safe for normal cells and analysis by using LC-MS/MS showed a benzazepine compoundin the ethanol extract of S. rhombifolia is known for its role as antiproliferation. These results indicate thatS. rhombifolia leaves extract has the potential to be developed as anticancer compounds..
RESUMEN
A espécie invasora guanxuma (Sida rhombifolia L.) é comum em áreas de plantio direto e destaca-se nacompetição com a cultura agrícola por apresentar um sistema radicular bastante eficiente; para tanto, seucontrole depende comumente de aplicações químicas em larga escala. O presente estudo teve comoobjetivo avaliar o efeito alelopático do extrato aquoso quente das espécies medicinais capim limão(Cymbopogon citratus Stapf.) e sabugueiro (Sambucus australis Cham & Schltdl.) sobre a germinaçãoda espécie invasora guanxuma. O experimento foi realizado em duas etapas complementares: primeiramentevisou-se à quebra de dormência da espécie invasora e depois, verificou-se o efeito de capim limão esabugueiro sobre a germinação de guanxuma. O acondicionamento das sementes foi realizado em placasde Petri previamente autoclavadas por 20 minutos a 121°C, dispostas em Câmara de Germinação comfotoperíodo de 12 horas a 25°C. Diante das condições em que foi realizado o experimento, identificou-sepossível efeito alelopático na germinação de sementes de guanxuma quando expostas ao extrato aquosoquente das duas espécies medicinais.
The invasive species Cuban jute (Sida rhombifolia L.) is common in no-till areas. It is an important competitor of crops because of a quite effective root system. For this reason, Cuban jute control commonly is made on large-scale applications of chemicals. This study seeks to make a contribution to the control of Cuban jute via allelopathy using hot aqueous extracts of the medicinal species lemongrass (Cymbopogon citratus Stapf.) and sabugueiro (Sambucus australis Cham & Schltdl.). The experiment was carried out in two complementary steps: first we attempted to overcome dormancy of the invasive species, and then we investigated the effect of the medicinal species lemongrass and sabugueiro on Cuban jute germination. The seeds were placed in Petri dishes previously autoclaved for 20 minutes at 121°C and then taken to a germination chamber with a photoperiod of 12 hours at 25°C. A potential allelopathic, inhibitory effect was identified on Cuban jute seed germination when the weed was exposed to the hot aqueous extract of both medicinal species.
Asunto(s)
Cymbopogon/crecimiento & desarrollo , Malvaceae , SambucusRESUMEN
OBJECTIVE:To improve the quality standard of Sida rhombifolia. METHODS: The microscopic structure of Sida rhombifolia was identified. RESULTS: The fiber bundles in circular permutation were apparent in bark area of the transverse section in stem and root. The main vein of the leaf assumed sulciform and the palisade tissues were in 2 tiers.There were large stellate hair,small bulb-shaped glandular hair,vase-shaped glandular hair and calcium oxalate cluster crystal in the powder.CONCLUSION: The experimental results serve as references for the identification and the establishment of the related quality standard for Sida rhombifolia.