RESUMEN
The FRUITFULL (FUL) gene belongs to the AP1/ FUL subfamily of the plant MADS-box family and has functions in regulating flowering time, floral meristem differentiation and fruit development. PfFUL gene sequence was derived from the perilla transcriptome data, and the basic physicochemical properties of PfFUL were analyzed by bioinformatics methods. Evolutionary relationships of PfFUL with other species of FUL were analyzed by phylogenetic tree. Plant expression vector 35S::PfFUL was constructed and used to transform wild type Col-0 and mutant ful-7 Arabidopsis to obtain overexpression 35S::PfFUL/ Col-0 and complemented mutation 35S::PfFUL / ful-7 plants respectively. Comparative phenotypic analysis was performed to preliminarily clarify the function of PfFUL gene in plant flowering and fruit development. The functions of the PfFUL gene during flowering and angular fruit development of the plants were initially clarified. The CDS of PfFUL gene is 738 bp and encodes 245 amino acids. Phylogenetic tree showed that the perilla PfFUL was closely related to Solanum lycopersicum, Salvia splendens and Salvia hispanica, but far related to Arabidopsis thaliana, Nicotiana tabacum and Vitis vinifera. Compared to Col-0 and ful-7, the transgenic plants showed early flowering (P0. 05), and the amount of wrinkled seed was significantly reduced (P<0. 01). In addition, phenotypic observations revealed that the transgenic plants also exhibited increased internode length and narrowed and curled cauline leaves. In conclusion, this study confirmed that the PfFUL gene regulates early flowering and fruit development in plants and participates in nutritional growth.
RESUMEN
ABSTRACT: The objective of this study was to adjust parameters related to the interception of photosynthetically active radiation (PAR) by reproductive structures of spring canola, with different nitrogen levels and hybrids. Two field experiments were conducted, being one with different sowing dates and hybrids (Hyola 61 and Hyola 432) and another with doses of 10, 20, 40, 80, 160kg ha-1 of N, applied in top dressing. They were conducted in Passo Fundo and Coxilha, RS, Brazil, in 2011 and 2014, respectively.The evaluated variables were: area index of reproductive structures, interception efficiency and extinction coefficient of these structures for PAR. PAR interception increased by increasing the reproductive structures volume. Interception efficiency of PAR by reproductive structures ranged from 45 to 80%. It was higher in the Hyola 61 hybrid and at the highest dose of N. The extinction coefficient of reproductive structures for PAR was 0.44 in non-limiting doses of N.
RESUMO: O objetivo deste estudo foi parametrizar a interceptação de radiação fotossinteticamente ativa (RFA) pelas estruturas reprodutivas de canola de primavera, em função de doses de nitrogênio e híbridos. Dois experimentos de campo foram conduzidos, sendo um com diferentes híbridos (Hyola 61 e Hyola 432) e outro com doses de N de 10, 20, 40, 80, 160kg ha-1, aplicadas em cobertura. Eles foram conduzidos em Passo Fundo e Coxilha, RS, Brasil, em 2011 e 2014, respectivamente. As variáveis avaliadas foram: índice de área de estruturas reprodutivas, eficiência de interceptação de RFA e coeficiente de extinção dessas estruturas. A interceptação de RFA aumentou com a elevação do volume das estruturas reprodutivas. A eficiência de interceptação de RFA pelas estruturas reprodutivas variou de 45 a 80%, sendo maior no híbrido Hyola 61 e na maior dose de N aplicado. O coeficiente de extinção de estruturas reprodutivas foi de 0,44 em doses não limitantes de N.