The Mitochondrial Apoptosis Pathway Is Involved in Testicular Apoptosis Induced by Cigarette Smoke Exposure in Rats / 中国生物化学与分子生物学报

In recent years, with the rapid increase of smoking in the world, male reproductive toxicity induced by cigarette smoking (CS) has attracted increasing attention. Studies have shown that long-term heavy smoking can lead to testicular damage in men, resulting in decreased semen quality, but the specific mechanism is still unknown. This study aimed to investigate the regulation mechanism of the Bcl-2 signaling pathway in rat testicular apoptosis induced by CS exposure. SPF male SD rats were randomly divided into high, medium and low CS exposure groups (30, 20 and 10 non-filtered cigarettes/ day, respectively) and control groups. The rats in each group were treated with static exposure methods and were anesthetized after 2, 4, 6, 8 and 12 weeks-CS exposure, respectively. The testicular organ coefficient was calculated, and the testicular histopathological changes and apoptosis in rats were detected. The mRNA and protein expressions of Apaf-1, Caspase-9, Bim, Bcl-w and Bak in the mitochondrial apoptosis pathway were detected. Results indicated that with the increase of exposure dose and duration, the weight of rats in exposure groups gradually decreased, the testis in exposure groups showed obvious pathological changes, and the testicular organ coefficient gradually decreased. Compared with the control groups, the testicular organ coefficient in the middle, high-dose groups at the 8th week and all groups at the 12th week significantly decreased (P 0. 05). In conclusion, long-term heavy smoking activated the mitochondrial apoptosis pathway and induced testicular irreversible apoptotic damage. These results provide a scientific basis for further study of the molecular mechanism of male reproductive injury induced by cigarette smoking.

Involvement of histone modifications in process of uterine injury alleviated by TSA in female mice induced by cigarette smoke exposure / 中国药理学通报

Aim To explore whether histone modifications are involved in the process of uterine injury alleviated by TSA in female mice induced by cigarette smoke (CS) exposure. Methods Female mice were exposed to CS twice daily for 30 days and TSA was injected intraperitoneally into CS-exposed mice on alternate days in the TSA-treated group. HE staining was used to observe the morphological changes of mice uterus after CS exposure; Western blot was used to assess the global modification levels of H3K4mel, H3K4me2, H3K4me3, H3K9mel, H3K9me2, H3K9me3 and H3K27me3 in uteri. GLP (H3K9 his-tone methyltransferase) , G9a (H3K9 histone methyl- transferase) , EZH2 (H3K27me3 histone methyltrans ferase ). Results TSA effectively restored the number of glandular and interstitial cells reduced by CS exposure. Western blot results showed that TSA significantly inhibited global H3K9mel modification and further aggravated H3K27me3 change induced by CS exposure. Furthermore TSA suppressed GLP and G9n expression in mouse uterine tissue induced by CS exposure, but further activated EZH2 increase. Conclusions Histone modifications are involved in the process of uterine injury alleviated by TSA in female mice induced by cigarette smoke exposure.

Passive smoking exposure and related risk awareness among adults in Zhengzhou / 预防医学

Objective @#To learn the passive smoking exposure and hazard awareness among the residents aged 18 years and over in Zhengzhou,so as to provide evidence for tobacco control. @*Methods@#By multi-stage stratified and clustered sampling method,the residents aged 18 years and over in Zhengzhou were selected. From June to October in 2018,a questionnaire for risk factors of non-communicable diseases,designed by Chinese Center for Disease Control and Prevention,was used to collect their passive smoking exposure and hazard awareness and then analyzed.@*Results @#A total of 6 793 questionnaires were qualified in 6 809 questionnaires and the effective rate was 99.77%. Among 5 387 non-smokers,2 131 people were exposed to passive smoking,and the crude and standardized rate was 39.56% and 40.14%. The standardized rate of passive smoking exposure was higher in men than in women（42.44% vs. 38.67%,P<0.05）,in rural residents than in urban residents（43.90% vs. 36.62%,P<0.05）,and it decreased with the age increase（P<0.05）. The standardized rate of passive smoking exposure in family,indoor workplace and indoor public place was 30.99%,36.99% and 68.02%,respectively. The standardized awareness rate of “passive smoking exposure may lead to heart disease,lung disease in children and lung cancer in adults” was 56.63%,which was higher in urban rural residents than in rural residents（75.69% vs. 36.33%,P<0.05）. @*Conclusions@#The passive smoking exposure rate was high in Zhengzhou,especially in indoor public places. The awareness of the hazards of passive smoking exposure was scarce, especially in rural residents.

Smoking Exposure and Placental Vascular Compromise: A Nationwide Population-Based Study in South Korea

PURPOSE: This study aims to investigate the effects of smoking on the development of placenta-associated syndromes, including preeclampsia, abruptio placentae, and placenta previa, which share the common pathophysiology of vascular compromise of the placenta. METHODS: A total of 966,629 pregnancies identified from the Korean National Insurance Claims Database and the National Health Information Database were analyzed from 2010 to 2014. The adjusted odds ratio and attributable risk of smoking for the development of placenta-associated syndromes, such as preeclampsia, placenta previa, and abruptio placentae, were analyzed. Maternal age, alcohol consumption, exercise habit, and economic status were controlled as confounding variables. A binary logistic regression model was used, and simple and multiple logistic regression analyses were performed. RESULTS: Among 966,629 pregnancies, 11.86% of women were ever smokers. Ever smokers had a higher risk of developing placenta previa (adjusted odds ratio, 1.23; 95% confidence interval [CI], 1.18–1.29; adjusted attributable risk, 18.70%). The adjusted odds ratio of developing placenta-associated syndromes in ever smokers compared to nonsmokers over the age of 35 years with a low economic status was 1.32 (95% CI, 1.18–1.47), with an adjusted attributable risk of 23.95%. CONCLUSION: The risk of developing placenta-associated syndromes, such as preeclampsia, placenta previa, and abruptio placentae, is high in ever smokers. Pregnant ever smokers who are >35 years and belong to the lower one-third of the economic division require special care to prevent the development of placenta-associated syndromes.

Analysis on passive smoking exposure in adults aged 40 years and older in China, 2014 / 中华流行病学杂志

Objective To describe and analyze the passive smoking exposure level in adults aged ≥40 years in China.Methods A total of 75 107 adults from 125 sites of COPD surveillance points in China were surveyed by electronic questionnaire during face to face interviews.After comprehensive weighting of the samples,the passive smoking exposure level was analyzed.Results The passive smoking exposure rate of the adults aged ≥40 years was 44.9％ (95％CI:42.3％-47.5％).The exposure rate was highest in those aged from 40 to 49 years (51.0％,95％CI:47.9％-54.1％) in term of age groups,in those with senior high school education level and above (48.6％,95％ CI:44.9％-52.2％) in term of education level,and in office workers (57.7％,95％CI:51.8％-63.7％) in term of occupation.The adults reporting passive smoking exposure every days in a week accounted for 27.7％ (95％CI:25.5％-29.8％),and those reporting household passive smoking exposure accounted for 28.3％ (95％ CI:26.2％-30.5％).Logistic regression analysis showed that the passive smoking exposure level was different in different age groups and occupation groups.Conclusion The passive smoking exposure level is still high in adults in China.Age and occupation are the influencing factors of passive smoking exposure level.

Analysis on passive smoking exposure in adults aged 40 years and older in China, 2014 / 中华流行病学杂志

Objective To describe and analyze the passive smoking exposure level in adults aged ≥40 years in China.Methods A total of 75 107 adults from 125 sites of COPD surveillance points in China were surveyed by electronic questionnaire during face to face interviews.After comprehensive weighting of the samples,the passive smoking exposure level was analyzed.Results The passive smoking exposure rate of the adults aged ≥40 years was 44.9％ (95％CI:42.3％-47.5％).The exposure rate was highest in those aged from 40 to 49 years (51.0％,95％CI:47.9％-54.1％) in term of age groups,in those with senior high school education level and above (48.6％,95％ CI:44.9％-52.2％) in term of education level,and in office workers (57.7％,95％CI:51.8％-63.7％) in term of occupation.The adults reporting passive smoking exposure every days in a week accounted for 27.7％ (95％CI:25.5％-29.8％),and those reporting household passive smoking exposure accounted for 28.3％ (95％ CI:26.2％-30.5％).Logistic regression analysis showed that the passive smoking exposure level was different in different age groups and occupation groups.Conclusion The passive smoking exposure level is still high in adults in China.Age and occupation are the influencing factors of passive smoking exposure level.

Survey of degree of passive smoking exposure and related risk awareness in adults in China, 2013 / 中华流行病学杂志

Objective To understand the passive smoking exposure level and related risk awareness in adults in China in 2013.Methods A face-to-face questionnaire survey was conducted in 179 570 adults selected through multistage cluster sampling from Chinese chronic disease and risk factors surveillance sample population (2013) in 302 surveillance sites.The effective sample size was 176 179 adults.After comprehensive weighting of the samples,the passive smoking exposure level and the awareness rate of related risks were analyzed.Results The passive smoking exposure rate was 52.8％ (95％CI:51.2％-54.4％) and the rate decreased with age (x2=515.8,P＜0.000 1).The exposure rate was highest in persons engaged in commercial services (61.6％,95％CI:58.6％-64.5％).The awareness rate of related risks was 67.9％ (95％CI:65.8％-69.9％).The awareness rate was higher in urban area (77.0％,95％CI:75.0％-79.1％) than in rural area (60.0％,95％CI:57.7％-62.4％),in males (69.8％,95％CI:67.8％-71.7％) than in females (65.9％,95％CI:63.7％-68.1％).The awareness rate of three related diseases in urban residents (49.8％,95％CI:47.3％-52.4％) were higher than that in rural residents (37.9％,95％CI:35.4％-40.5％);the awareness rate of passive smoking related lung cancer was highest (88.5％,95％CI:87.5％-89.5％),followed by lung disease in children (70.0％,95％ CI:68.1％-71.9％),the awareness rate of passive smoking related heart disease was lowest (46.8％,95％CI:44.6％-49.1％).Conclusion The passive smoking exposure level is relatively high in adults in China,and the awareness rate of passive smoking exposure risks,especially heart disease,is low.

Survey of degree of passive smoking exposure and related risk awareness in adults in China, 2013 / 中华流行病学杂志

Objective To understand the passive smoking exposure level and related risk awareness in adults in China in 2013.Methods A face-to-face questionnaire survey was conducted in 179 570 adults selected through multistage cluster sampling from Chinese chronic disease and risk factors surveillance sample population (2013) in 302 surveillance sites.The effective sample size was 176 179 adults.After comprehensive weighting of the samples,the passive smoking exposure level and the awareness rate of related risks were analyzed.Results The passive smoking exposure rate was 52.8％ (95％CI:51.2％-54.4％) and the rate decreased with age (x2=515.8,P＜0.000 1).The exposure rate was highest in persons engaged in commercial services (61.6％,95％CI:58.6％-64.5％).The awareness rate of related risks was 67.9％ (95％CI:65.8％-69.9％).The awareness rate was higher in urban area (77.0％,95％CI:75.0％-79.1％) than in rural area (60.0％,95％CI:57.7％-62.4％),in males (69.8％,95％CI:67.8％-71.7％) than in females (65.9％,95％CI:63.7％-68.1％).The awareness rate of three related diseases in urban residents (49.8％,95％CI:47.3％-52.4％) were higher than that in rural residents (37.9％,95％CI:35.4％-40.5％);the awareness rate of passive smoking related lung cancer was highest (88.5％,95％CI:87.5％-89.5％),followed by lung disease in children (70.0％,95％ CI:68.1％-71.9％),the awareness rate of passive smoking related heart disease was lowest (46.8％,95％CI:44.6％-49.1％).Conclusion The passive smoking exposure level is relatively high in adults in China,and the awareness rate of passive smoking exposure risks,especially heart disease,is low.

Effect of cigarette smoking-induced plasma nicotine and cotinine on male rats / 中国实验动物学报

Objective To explore the in vivo exposure levels of cigarette smoking ( CS) by measuring the biomar-kers nicotine and cotinine. Methods One hundred and sixty male SD rats were divided into 15 cigarette exposure groups (10, 20 and 30 nonfilter cigarettes/day, for 2, 4, 6, 8 and 12 weeks) and a control group (without CS exposure). The rats were sacrificed at different time?points. The concentration of plasma nicotine and cotinine were measured by GC?MS/MS. Results The CS?exposed rats displayed decreased locomotor activity, ataxic gait, irregular respiration, nasal noise, and salivation after smoking exposure for 3 weeks. Rats in the CS exposure groups had lower body weight, and the reduction of body weight was time and dose related (P<0. 01). The retention time of nicotine was 7. 5 to 8. 5 min. The concentra?tion of plasma nicotine in the CS exposure groups was higher than control group (155 ± 56. 65) ng/mL. The retention time of cotinine was 11. 5 to 12. 5 min, the concentrations of plasma cotinine in CS exposure groups were higher than control group (340 ± 41. 97) ng/mL, the increase of plasma cotinine in CS groups was time?related (P<0. 05), and the exposure concentration and duration had synergistic effect on the level of plasma cotinine ( P<0. 05 ) . Conclusions CS exposurecauses structural damages in male rats. The plasma concentration of cotinine can effectively reflect the in vivo exposure lev?els of cigarette smoking, and well presents a dose?response relationship.

Oxidative stress and inflammatory changes in the lung caused by cigarette smoking exposure in mice and the effect of smoking cessation / 安徽医科大学学报

Objective To observe the changes of pulmonary oxidative stress after cigarette smoking exposure,its re-lationship with inflammatory cytokines,and the effects of smoking cessation. Methods Fifty male BALB / c mice were randomly divided into the smoke exposure group,smoke cessation group,and the controls. Mice in smoke cessation group were exposed to cigarette smoking for 16 weeks. On 4,8,and 12 week after smoking cessation mice were executed and the bronchoalveolar lavage fluid(BALF)and lung tissue were collected. The morphologi-cal alternations of lung tissue were observed. Mean length of interval and mean alveolar number were measured. Total cell numbers in BALF were counted. Superoxide dismutase(SOD)activity was measured with hydroxylamine method,malondialdehyde(MDA)level was measured with TBA method. The levels of pulmonary interleukin-8 (IL-8)and tumor necrosis factor-α(TNF-α)in BALF and lung tissue homogenate were measured with ELISA. Re-sults Compared with the mice in the controls,emphysematous changes were remarkable in the lung of cigarette ex-posed mice,the total cell numbers in BALF were increased significantly(P < 0. 05)and reduced gradually after smoking cessation(P < 0. 05). SOD and MDA levels increased remarkably in the cigarette exposure group(P <0. 05),and declined gradually after smoking cessation. The levels of IL-8 and TNF-α in BALF and lung tissue ho-mogenate in the smoke exposure group increased significantly( P < 0. 05),and lowered time-dependently after smoking cessation,but not reached to normal level even 12 weeks after smoking cessation. SOD and MDA levels were positively correlated with the cytokine changes. Conclusion Abnormal oxidative stress in the airways caused by cigarette smoking exposure was merely partially reversed after smoking cessation. And the inflammation remains persistent concomitantly.