RESUMEN
Objective: To establish the introduction and culture system of the hairy roots in Solanum lyratum and to screen the clone of hairy roots with more diosgenin. Methods: The explants of S. lyratum were infected by Agrobacterium tumefaciens strain C58C1, to obtain the hairy roots and construct the genetic transformation system of the hairy roots in S. lyratum. HPLC was used to determine the diosgenin in the hairy roots. Results: The optimum transformation results were obtained with the max inductivity of hairy roots of 83.33% during infecting time for 10 min by C58C1 and co-cultural time of 4 d. The average content of diosgenin in the hairy roots was 4.620 mg/g, it was 2.652 times as high as that in the leaves (1.742 mg/g) which had the highest diosgenin content in the different tissues of wild type plant of S. lyratum. Conclusion: It is an effective way to obtain diosgenin from the hairy roots of S. lyratum.
RESUMEN
The present study was aimed at the comparison of the pharmacokinetics of pure chlorogenic acid and extract of Solanum lyratum Thunb. The animals were allocated to two groups, and were administered chlorogenic acid or extract of S. lyratum Thunb. at a dose of 50.0 mg/kg orally. Blood samples were collected up to 8 h post-dosing. Plasma chlorogenic acid analyses were performed using an HPLC method with UV detector. The pharmacokinetic parameters were evaluated using non-compartmental assessment. Significant differences existed in the two groups for AUC0-t, AUC0-∞ and CLz/F. The reliable HPLC method was successfully applied to the determination of chlorogenic acid in rat plasma at dosage of 50.0 mg/kg.
RESUMEN
A rapid method for the simultaneous determination of daidzein, genistein and formonetin in solanum Lyratum Thunb by high performance liquid chromatography (HPLC) was developed. Separation was achieved on a Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with isocratic elution, using a mobile phase of methanol-tetrahydrofuran-water (44∶3∶53, v/v). The wavelength was set at 260 nm and column was maintained at 35 ℃. The linear ranges of daidzein, genistein and formonetin were 1.0-40.0, 0.1-4.0 and 0.1-4.0 μg/mL, respectively. The average recoveries were between 98.4% and 101.3%. This method could be used for the quality control of Solanum lyratum Thunb due to its simplification, reliability, rapidity and excellent precision.
RESUMEN
A rapid method for the simultaneous determination of daidzein, genistein and formonetin in Solanum Lyratum Thunb by high performance liquid chromatography (HPLC) was developed. Separation was achieved on a Diamonsil C18 column (250 mm X 4.6 mm, 5 μm) with isocratic elution, using a mobile phase of methanol-tetrahydrofuran-water (44:3:53, v/v). The wavelength was set at 260 nm and column was maintained at 35°C. The linear ranges of daidzein, genistein and formonetin were 1.0-40.0, 0.1-4.0 and 0.1-4.0 μg/mL, respectively. The average recoveries were between 98.4% and 101.3 %. This method could be used for the quality control of Solanum lyratum Thunb due to its simplification, reliability, rapidity and excellent precision.
RESUMEN
OBJECTIVE:To explore the effects of ethanol extracts of Solanum lyratum Thunb(ST) on induction of apoptosis and the expression of apoptosis associated genes fas and caspase-3 in human lung cancer SPC-A-1 cells.METHODS:Cultured human lung cancer SPC-A-1 cells were randomly divided into the control group,ethanol extracts of ST treated groups(2.5、5、10 mg?L-1)and the positive control group(cisplatin).After treatment with drug for 48 h,the proliferation inhibitory rate was evaluated by MTT assay,induction of cell apoptosis rate was determined by TUNEL method,the expression of fas and caspase-3 mRNA was detected by semi-quantitive RT-PCR.RESULTS:Compared with control group,the inhibitory rate was increased obviously(P