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Objective To research the expression of Sp3 andβ‐Catenin in HCC and study the assessable factors of them for prognosis in patients with hepatocellular carcinoma .Methods Western blot and RT‐PCR methods were used to detect the expres‐sion of Sp3 andβ‐Catenin in HCC and the liver tissue beside tumor among 49 cases .We analyzed the difference of these two indexes expressed in HCC and the liver tissue beside tumor .Then we detected the correlation between these two indexes and the character of clinic pathology ,and researched the correlation between Sp3 and the prognosis of HCC .Results The high expression rate of Sp3 in HCC was higher than that of liver tissue beside tumor(P<0 .05) according to Western blot and RT‐PCR ,the same toβ‐Catenin (P<0 .05) .Expression of Sp3 andβ‐Catenin were both related with size of tumor and degree of differentiation .Positive correlation existed between these two indexes according to Western blot method(r=0 .681 ,P=0 .000) and RT‐PCR method(r=0 .641 ,P=0 .000) .The prognosis of cases with high expression of Sp3 was poorer than the low expression cases(P<0 .05) .Conclusion Sp3 plays a promoter role in occurrence of HCC ,which is correlated with the grade malignancy of HCC .Sp3 might participated in occur‐rence and development of HCC via the Wnt pathway .
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<p>The aim of this study was to investigate the effects of acupuncture to SP 3 on the quadriceps femoris during knee extension in patients with musculoskeletal diseases by applying the meridian concept.<br>The subjects included 10 healthy individuals with a mean age of 23.1 years, and all of whom provided informed consent for participation. During knee extension, each subject underwent three different types of acupuncture stimuli : 1) stimulation to SP 3, 2) stimulation to SP 4, and 3) no stimulation. For each stimulation,the subjects performed isometric contraction with 40% of maximum voluntary contraction with knee flexion to 60°. Surface electromyography (EMG) of the vastus medialis obliquus, vastus medialis longus, rectus femoris,and the four sites within the vastus lateralis was performed during knee extension before acupuncture stimulation ; immediately after starting stimulation ; and 5, 10, and 15 min after starting stimulation. Relative-integrated EMG data was recorded during and after acupuncture stimulation and compared to data that were recorded before acupuncture as reference values.<br>The results showed that after 15 min of acupuncture stimulation to SP 3, the relative-integrated EMG data on the vastus medialis obliquus was significantly increased compared to the data obtained without stimulation (p < 0.05).<br>Therefore, in conclusion, after 15 min of acupuncture stimulation to SP 3, the relative-integrated EMG data on the vastus medialis obliquus exhibited suppressed muscle function.</p>
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Objective To evaluate the expression of Sp3 gene of peripheral blood mononuclear cells (PBMC) in multiple sclerosis (MS) patients in Guizhou and the relationship between Sp3 gene expression and immunological function. Methods Two pairs of primers were used to amplify cDNAs generated from 31 MS patients and 30 healthy controls. The serum levels of sIL-2R were measured in 27 patients with MS and 30 healthy controls by sandwiched ELISA. Results The deficient expression of Sp3 gene in MS patients was significantly higher than that in control (41.9% ( 12/31 ) vs 6. 7% (2/30) ,x2 =7. 133 ,P =0. 008). The sIL-2R levels in MS patients were significantly higher than those in control (( 2788.5 ± 1079. 8 ), ( 1270. 7 ± 489. 4) μg/L, t = 6. 170, P = 0. 001 ). The concentration of sIL-2R in MS with negative ((3364.0 ± 1252.3) μg/L) and positive((2450.0 ± 827.0) μg/L) expression of Sp3 gene were significantly increased compared with control (F = 32. 059, P < 0. 05 ). The sIL-2R levels were significantly rising in MS patients with negative expression of Sp3 gene compared with MS patients with positive expression of Sp3 gene ( q = 4. 213, P < 0. 05 ). Conclusions A remarkable deficient expression of Sp3 gene in PBMC has been found in MS patients in Guizhou. sIL-2R may take part in the process of MS. The expression of Sp3 gene is not affected by immune state, however, MS patients with Sp3 deficient expression tend to have a more serious impairment in immunological functions.
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By NAMBA ‘weight method from leaf, root and stem of Schefflera sp. triterpen saponins were qualified. By thin layer chromatography, 11 stains of saponin were determined on leaf, 7 on stem and root with the quantities of 3,66%, 0,93%, 0,77% in absolutely dried leaf,stem and root, respectively. By thin layer chromatography 4 stains of total sapogenins of the leaf and 2 genins were isolated on column chromatography. Fusion points,chromatography,IR spectrum, 1H-NMR,13 C-NMR spectrography,DEPT,HMQC and HMBC had showed oleanolic and hederagenin in the structure of 2 genins
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Saponinas , Araliaceae , SapogeninasRESUMEN
The stems and roots of Schefflera sp3 contained numerous fibers with cubic calcium oxalate crystals, lignified medullary rays. The leaves were covered with protective multicellular hairs in the shape of ramified candles. Saponin triterpenes, phytosterols, reduced sugars, tannin, coumarins, organic acids and uronic compounds were found in the leaves, stems and roots. Of the unsaturated fatty acids, oleic and linoleic acids were found in high amount in these 3 parts of the plant, but linolenic acids only in the leaves. The composition of oligoelements were similar in the 3 parts, except Cr only in the leaves, Ga only in the roots, whereas Ni, Mo, Sn and Pb only in the stems.
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Botánica , Química , Plantas MedicinalesRESUMEN
ATP-citrate lyase (ACL), an enzyme catalyzing the first step in biosynthesis of fatty acids, is induced during the lipogenesis and cholesterologenesis. We demonstrate that the region -213 to -128 of human ACL promoter is responsible for conferring glucose-mediated transcription. This region in the ACL promoter contains Sp1 binding sites determined by DNase I foot-printing assay. Gel retardation assay using oligonucleotides from -179 to -141 and -140 to -110 showed two specific DNA-protein complexes postulated to be formed by transcription factor Sp1. Competition gel shift and supershift assays have confirmed that these DNA-protein complexes were the result of induced Sp1 as well as another Sp1-related proteins. Western blot analysis also demonstrated that transcription factor Sp1 was slightly increased in the nuclear proteins extracted from Alexander cells following supplementation of glucose. In addition, expression of 110 kDa protein reacting with antibody against Sp3 was dramatically increased by glucose supplementation, while isoforms of Sp3, about 80 kDa in size was decreased in its amounts. Our results suggest that changes in the expression of Sp1 family proteins play an important role in activation of the ACL promoter by glucose.