RESUMEN
The melamine molecular imprinted polymer ( MIP ) membrane immobiliZed on the surface of the surface plasmon resonance ( SPR) chip was prepared with allylcyanurate and ethylene glycol dimethacrylate as a functional monomer and cross-linker, respectively.Based on the ratio of the intercept to the slope of Langmuir equation for imprinted polymer, the association constant ( Kass ) of imprinted sites in the imprinted film to melamine was calculated to be 1.40 × 108 L/mol.The SPR sensor showed high selectivity to the template molecule melamine, and the best response was obtained at pH=7.5.The method showed good linearity in the melamine concentration range of 0.1-10.0 nmol/L ( R=0.9991 ).Based on a signal to noise ratio of 3, the detection limits of melamine were 62.6 pmol/L and 56.4 pmol/L for the milk and milk powder, respectively.The recoveries were 91.5% for milk and 92.0% for milk powder.These results suggest that SPR sensing combined with MIP film is a promising alternative method for detection of melamine.
RESUMEN
Surface plasmon resonance (SPR) sensor technology,with its features of real-time,fast,no need for labeling,no background interference and non-destructive to samples,etc.,has been widely used in the field of biotechnology,medicine,environmental science and drug detection.This article gives an overview of the recent popular studies and their progress in SPR technology is reviewed,especially giving a detailed overview of the surface modification technique,related hyphenated techniques and application of SPR in clinical examination.Hyphenated techniques is discussed from three aspects of molecular imprinting technique,SPR based immunoassay and nucleic acids SPR biosensor as well.