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OBJECTIVES@#To estimate the system efficiency of uncle-nephew relationship identification by increasing STR markers and adding reference samples based on the test results of simulated data and real samples, so as to provide references for selecting the appropriate number of STRs and reference samples for uncle-nephew relationship identification.@*METHODS@#Five common models of uncle-nephew relationship identification were constructed by adding different reference samples. In each model, the likelihood ratio (LR) for 10 000 pairs of uncle-nephew relationships and 10 000 pairs of unrelated individuals were simulated by detecting 19, 39 or 55 STRs, and the system efficiency at different thresholds was simulated. The samples of the Han population in Zhejiang were collected, and 55 autosomal STRs were obtained by using SiFaSTRTM 23plex kit, Goldeneye® DNA ID 22NC kit and AGCU 21+1 PCR amplification kit. When 19, 39 and 55 STRs were detected, the LR of each model and system efficiency under different thresholds were calculated and compared with the simulation results.@*RESULTS@#Under the same detection system, the calculated results of simulated data and corresponding true samples were basically consistent. In the same model, there was a positive correlation between the system efficiency of uncle-nephew relationship identification and the number of STRs detected. Moreover, the system efficiency of introducing relatives was higher than identifying only two individuals. The order of preference for the introduction of relatives was the full sibling (or mother) of the uncle and the full sibling (or mother) of the nephew.@*CONCLUSIONS@#The system efficiency of uncle-nephew relationship identification could be improved by increasing the number of STRs and introducing known relatives, which would provide the basis for selecting the most appropriate detection system and reference individuals in actual cases.
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Humanos , ADN , Dermatoglifia del ADN , Repeticiones de Microsatélite , Reacción en Cadena de la Polimerasa , HermanosRESUMEN
RESUMO O coeficiente de retorno (f) da rede coletora de esgoto, que apresenta quociente dado pelos volumes água/esgoto micromedidos, é empregado no projeto e na gestão do sistema de coleta de esgoto sanitário. As fontes de suprimento de água alternativas, tais como águas de reúso e de aproveitamento pluvial, não estão contempladas por f. O presente artigo teve o objetivo de aplicar metodologias para avaliar o potencial do lançamento de água pluvial no sistema de coleta e transporte de esgoto sanitário por meio de práticas de aproveitamento, na cidade de Bauru/SP. As variáveis de interesse foram parametrizadas utilizando o número de habitantes. Foi usado procedimento amostral para a determinação das áreas de telhado disponíveis. Os resultados apontam para correções de f (f corrigido ) na faixa de 0,86 e 1,14.
ABSTRACT The return coefficient (f) of the sewage disposal system, which presents the ratio given by the volume of wastewater by the public supply system water volume is employed in the design and management of sanitary sewer collection system. The use of alternative sources, such as reuse and rainwater harvesting, are not considered by f. This article aims at applying the methodology to evaluate the potential release of rainwater in the collection and transportation of sanitary sewage system through recovery practices in Bauru city/SP. Variables were quoted by means of number of inhabitants. The statistical sampling procedure was applied for roof area determination. Results pointed out for f correction (f corrected ) values varying from 0,86 to 1.14 for Bauru city.
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Objective:Analyzing the efficiency of medical system in China and examining the impact of the imbalance of regional health system efficiency on the health status of residents.Methods:Using DEA method to measure the output efficiency of inter-provincial medical system in China;employing the panel quantile regression model to measure the health improvement effect and changing trend brought by the promotion of the efficiency of medical system.Results:The efficiency of health systems in different provinces in China showed the situation of high in the east and low in the west,regional imbalances were obvious;the impact of the promotion of efficiency of the medical system on the residents' health status would be weakened with improvement of residents' health status.In areas where the residents' health status was the best,the effect of improving the efficiency of medical treatment had no significant.Conclusion:It should give full play to improvement effect of medical system efficiency on the residents' health,focus on supporting the construction of medical systems in backward areas to promote the balanced development of China's regional medical system.
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Na cultura da soja, o déficit hídrico é um dos fatores limitantes à obtenção da máxima produtividade. A identificação de genótipos com capacidade de tolerância à seca é fundamental para a solução desse problema. Objetivou-se, neste trabalho, analisar a qualidade fisiológica e as respostas bioquímicas de cultivares de soja (MG/BR 46 Conquista, UFUS Carajás, UFUS Impacta, UFUS Riqueza e UFUS Xavante) submetidas ao estresse hídrico com soluções de PEG 6000 em diferentes níveis de potencial osmótico (0; -0,1; -0,2; -0,3 e -0,4 MPa). Avaliou-se a qualidade fisiológica das cultivares por meio dos testes de comprimento de plântulas e biomassa fresca e seca de plântulas. As respostas bioquímicas foram avaliadas pela detecção do ácido indol-acético, pelo conteúdo de sacarose e pelo transporte de água nas plântulas. A cultivar UFUS Riqueza apresentou os melhores desempenhos nos testes de qualidade fisiológica, conteúdo de sacarose, absorção e velocidade de absorção de água em plântulas. A cultivar UFUS Xavante mostrou a maior concentração de AIA e o maior massa de plântulas no teste de transporte de água.
In soybean culture water deficit is one of the most limiting factors to the maximum yield obtained. Genotypes identification with drought tolerance capacity is fundamental to solve this problem. The objective in this paper was analyze the physiological quality and physiological and biochemical responses of soybeans cultivars (MG/BR 46 Conquista, UFUS Carajás, UFUS Impacta, UFUS Riqueza and UFUS Xavante) submitted to water stress with PEG 6000 solutions in different levels of osmotic potentials (0; -0,1; -0,2; -0,3 and -0,4 MPa). Physiological quality of cultivars were evaluated by seedling length test and fresh and dry biomass. The biochemical responses were evaluated by detection of indol-acetic acid (IAA), by saccharose content and seedling water transportation. UFUS Riqueza presented the best performance in physiological quality tests, saccharose content, absorption and water absorption velocity in seedling. UFUS Xavante showed the highest IAA's concentration and the greater weight of seedling in water transportation test.