Synergistic effect of sorafenib with Platycladus orientalis (L) leaf extract on cervical cancer

The extracts of Platycladus orientalis (L.) Franco leaves have shown promising anti-cancer, anti-oxidant and anti-inflammatory potency with the traditional knowledge of healing HPV associated warts. The purpose of this research is to assess the synergistic activity of sorafenib and Platycladus orientalis (L) leaf extraction on cervical cancer cells. The cytotoxicity efficiency of different concentrations of Sorafenib and ethanol extract of Platycladus orientalis (L.) leaves were tested on HeLa cells by MTT and Trypan blue assays. The synergistic effect of the IC50 concentrations of Sorafenib and Platycladus orientalis (L.) on HeLa cell by MTT assay, and mRNA expression levels of tumor suppressor tazarotene-induced gene 3 (TIG3), proliferating cell nuclear antigen (PCNA) gene and apoptosis modulator (Bcl-2) gene by RT-PCR were evaluated with individual treatments. Combination treatment showed a relatively more expression of TIG3 and less expression of Bcl-2 and PCNA was observed. Growth factor-induced MAPKP activation was arrested by compound combination treatment, which and suppression of proliferation-induced apoptosis of cervical cancer cells. Based on the our results, the combination of sorafenib and crude leaf extract from Platycladus orientalis (L.) can effectively suppress cervical cancer cell growth, thereby providing an interesting rationale for further clinical trials and in-vivo studies.

Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee

BACKGROUND: Chemerin, encoded by the retinoic acid receptor responder 2 (RARRES2) gene is an adipocytesecreted protein with autocrine/paracrine functions in adipose tissue, metabolism and inflammation with a recently described function in vascular tone regulation, liver, steatosis, etc. This molecule is believed to represent a critical endocrine signal linking obesity to diabetes. There are no data available regarding evolution of RARRES2 in non-human primates and great apes. Expression profile and orthology in RARRES2 genes are unknown aspects in the biology of this multigene family in primates. Thus; we attempt to describe expression profile and phylogenetic relationship as complementary knowledge in the function of this gene in primates. To do that, we performed A RT-PCR from different tissues obtained during necropsies. Also we tested the hypotheses of positive evolution, purifying selection, and neutrality. And finally a phylogenetic analysis was made between primates RARRES2 protein. RESULTS: RARRES2 transcripts were present in liver, lung, adipose tissue, ovary, pancreas, heart, hypothalamus and pituitary tissues. Expression in kidney and leukocytes were not detectable in either species. It was determined that the studied genes are orthologous. CONCLUSIONS: RARRES2 evolution fits the hypothesis of purifying selection. Expression profiles of the RARRES2 gene are similar in baboons and chimpanzees and are also phylogenetically related.

Expression and clinical significance of TIG1 gene in acute leukemia / 白血病·淋巴瘤

Objective To investigate correlation between the expression of TIG1 and the prognostic significance in acute leukemia (AL).Methods The TIG1 expression level of 75 AL and 20 normal control (NC) were measured by Real-Time Quantitative PCR(RT-QT-PCR).Results TIG1 gene expressed at a higher level in NC(0.0609±0.0281 ) than that in de novo AL(0.0057±0.0035)(U=-6.321,P=0.000).The expression of TIG1 in complete remission patients(AL-CR)(0.0409±0.0244)were lower than that in NC too(U=-2.582,P=0.01).The expression of TIG1 in AL-CR patients (83.3 ％) were higher than that in de novo AL (53.1％)(U=-6.366,P=0.000).In AL patients,the CR rate of high TIG1 level cases were higher than that in low level cases (x2=4.563,P=0.033).Conclusions Reduced expression of TIG1 may play an important role in the pathogenesis of AL.TIG1 can serve as a marker of poor prognosis in AL.

Avaliação comparativa de propriedades geradas pelos processos de soldagem TIG e brasagem em uma liga odontológica à base de Ni-Cr / Comparative assessment of properties generated by the process of TIG welding and brazing in a Ni-Cr dental alloy

O objetivo deste trabalho foi comparar, por meio de teste de microdureza e técnicas de caracterização metalúrgica, que inclui a metalografia, microscopia óptica, microscopia eletrônica de varredura (MEV) e espectroscopia por dispersão de energia (energy dispersive spectroscopy – EDS), propriedades mecânicas e metalúrgicas de juntas soldadas pelos métodos TIG e brasagem em uma liga odontológica à base de Ni-Cr (FIT-CAST SB). As regiões estudadas em cada amostra foram o metal de base (MB), a zona termicamente afetada (ZTA) e a zona de fusão (ZF). Foram confeccionados 40 corpos-de-prova cilíndricos, em forma de bastão, com 2,5mm de diâmetro e 18,0mm de comprimento, obtidos apartir de fundição odontológica por centrifugação (técnica da cera perdida). Foram separados vinte cilindros para cada técnica, que após a soldagem formaram grupos de dez amostras para cada procedimento. A microestrutura do MB apresentou-se como uma matriz de uma solução sólida à base de Ni-Cr contendo um típico arranjo dendrítico de estrutura eutética. A liga apresentou precipitados metálicos na região interdendrítica da estrutura eutética. A análise química semi-quantitativa obtida através da técnica de EDS apresentou resultados compatíveis com a composição química quantitativa fornecida pelo fabricante da liga. Na brasagem, o MB e a ZF apresentaram microestruturas distintas. A ZF apresentou uma morfologia dendrítica grosseira com a presença de precipitados e porosidades. A análise química semi quantitativa por EDS da ZF mostrou-se bastante semelhante aos resultados obtidos para o MB...

The purpose of this study was to compare, by means of microhardness testing and metallurgical characterization techniques as metallography, optical microscopy, scanning electron microscopy (SEM) and energy dispersion spectroscopy (EDS), mechanical and metallurgical properties of welded joints by TIG and brazing methods in a dental alloy based on Ni-Cr (FIT-CAST SB). The studied regions in each sample were the base metal (MB), the heat affected zone (ZTA) and the fusion zone (ZF). Forty cylindrical bodies-of-proof, stick-shaped, with 2.5mm diameter and 18.0mm in length were made from dental casting by centrifugation (lost wax technique). Twenty cylinders were used for each technique, which formed, after the welding, groups of ten samples for each procedure. The microstructure of the MB is presented as a matrix of a solid solution based on Ni-Cr with a typical dendritic arrangement of eutectic structure. The alloy presented metallic precipitates in the interdendritic region of the eutectic structure. The semiquantitative chemical analysis obtained by EDS technique showed results consistent with the quantitative chemical composition provided by the alloy manufacturer. In the brazing method, the MB and ZF showed different microstructures. The ZF showed a rough dendritic morphology with the presence of precipitates and porosities. The semiquantitative chemical analysis by EDS of the ZF had very similar results to those obtained for the MB...

Hypermethylation of TIG1 in Head and Neck Cancer / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Decreased expression and growth suppression of Tazarotene-Induced Gene 1 (TIG1) were reported in prostate cancer. In this study, we examined the possibility of TIG1 transcriptional silencing by hypermethylation in head and neck cancer. SUBJECTS AND METHOD: We extracted DNA and total RNA from five head and neck cell lines (O11, O12, O19, O22 and O28), and three prostate cell lines (PC3, LNCap and DU145). We checked the methylation status of TIG1 by methylation-specific PCR (MSP) and RT-PCR. We also examined primary cancer tissues of 32 head and neck cancer, 31 prostate cancer and 10 normal samples. RESULTS: We found that all of five head and neck (100%) cell lines and two of three prostate (66.7%) cell lines were methylated. RT-PCR analysis confirmed the absence of TIG1 expression in six cell lines with methylation. We checked primary cancer by MSP and found TIG1 methylation in 16 of 32 (50%) head and neck cancers, and 17 of 31 (54.8%) prostate cancers. Normal head and neck and prostate tissues were free of methylation. CONCLUSION: Our results support the notion that methylation might be an important mechanism of TIG1 inactivation and a target event in head and neck cancer.