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Objective To evaluate the effect and mechanism of non-steroidal anti-flammatory drags(NSAIDs) on the colon cancer cell growth by using S-nitrosoglutathione(GSNO) which can produce nitric oxide.(Methods) Apoptosis of 3 colon cell lines were evaluated by cell growth curve and flow cytometry,the PGE_2 levels in cell culture supernatants were determined by competitive enzyme immunoassay method,and the(protein) expression of COX-1 and COX-2 were analyzed by Western blot.Results The production level of PGE_2 was increased with CSNO treated concentration and time.Using 500?mol/L CSNO treatment for 48h,the expression level of COX-1 and COX-2 protein increased.NASIDs can block the production of PGE_2 but had no effect on the inhibition of cell growth induced by GSNO.Conclusions GSNO can increase PGE_2(production) and induce COX-1 and COX-2 protein expression in a dose-and time-dependent manner.Higher concentrations of GSNO also can inhibite cell growth and induced apoptosis in all 3 cell lines.NSAIDs can block production of PEG2 but NSAIDs are no effect on cell growth.
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Objective To investigate the influence of ?-interferon (?-IFN) on liver cancer cell line (Hep-G2). Methods Observing the expression of Fas and Bcl-2 by ?-IFN-pretreated Hep-G2 cells via immunohistochemical stain; subsequently treating these cells with adrimysin, and observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results (1) ?-IFN up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P