RESUMEN
@#The chemical constituents of the CHCl3 extracts from the leaves and stems of Taxus wallichiana var. mairei were investigated. Twelve taxane diterpenoids were isolated and identified by spectroscopic analysis as 2-deacetoxytaxinine E (1),2-deacetoxytaxinine J (2),7-deacetoxytaxinine J (3),taxinine J (4),7,2′-didesacetoxyaustrospicatine (5),N-methyltaxol C (6),2-deacetoxydecinnamoyl taxinine J (7),taxol (8),7-epi-taxol (9),7-epi-10- deacetoxytaxol (10),cephalomannine (11),7-epi-cephalomannine (12). Compounds 1 and 3 were isolated from the leaves and stems of Taxus wallichiana var. mairei for the first time.
RESUMEN
Objective: To study the chemical constituents from the petroleum ether and ethyl acetate extract of the twig and leaves of Taxus media. Methods: Several column chromatography including silica gel, Sephadex LH-20 gel, ODS and so on were applied in isolation and purification. The structures were elucidated on the basis of physicochemical properties and spectral data. Results: Eight taxane diterpenoids, five flavonoids, three phenolic acids, and two other compounds were isolated and determined as taxinine-11,12-oxide (1), 2-deacetoxytaxuspine C (2), 2-deacetoxytaxinine E (3), 7,9-deacetyltaxinine B (4), taxagifin (5), 9-deacetyltaxinine A (6), 9-deacetyltaxinine (7), 10-desacetylbaccatin III (8), kaempferol (9), aromadendrin (10), apigenin (11), sciadopitysin (12), ginkgetin (13), 4-hydroxy-benzaldehyde (14), p-hydroxybenzoic acid (15), pyrocatechol (16), β-sitosterol (17), and eicosan-10-ol (18), respectively. Conclusion: Compounds 1-4, 6, 7, 10, 14-16 are firstly isolated from the plant of T. media, and compound 18 is obtained from the Taxaceae for the first time.
RESUMEN
Objective: To investigate some factors which could influence the tissue culture of callus from Taxus chinensis var. mairei such as the culturing callus time, callus induction rate, callus development rate, growth rate, and browning degree. Then, the five taxane diterpenoids including paclitaxel in callus were studied and compared. Methods: The factors which influence the T. chinensis var. mairei callus induction and growth rate were studied by single factor analysis and orthogonal test design. The five taxane diterpenoids including paclitaxel in callus from different sources were determined by HPLC analysis. The weighted score for each of the above factors mentioned would be taken account to optimise the tissue culture conditions. Results: Among the explants, the stems intacted leaves would exhibit the best ability of dedifferentiation to form callus. The enrichment dark culture was conducive to paclitaxel and other five kinds of terpene constituents. The most optimum culture medium of the callus induction was B5 + 2, 4-D 3.0 mg/L + 6-BA 1.0 mg/L + NAA 1.0 mg/L + KT 0.5 mg/L. Conclusion: The callus culture conditions for T. chinensis var. mairei have indicated greater association with different types of the explantation, nutrient medium, and light illumination. The five kinds of enrichment taxane paclitaxel diterpenoids would be significantly affected by the type and concentration of the plant growth regulators. This study has shown some references value for T. chinensis var. mairei tissue culture and for the screening of high-yielding cell lines.