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Objective:To explore the effect of processing methods on the content of toddaline in Toddalia asiati- ca Lam., so as to provide reference for the development and clinical application of T. asiatica in the future. Methods: The root of T. asiatica was processed by the processing methods of water-washing, water-boiling, water-steaming, winesteaming and stir-frying with wine. The content of toddaline in the processed products of T. asiatica was determined by high performance liquid chromatography(HPLC). Further, the orthogonal test design methodology was used for the stirfrying with wine process to investigate the effect of frying fire power, time and solid-liquid ratio(the ratio of raw material to yellow rice wine)on the toddaline content in T. asiatica. Results: The processing of the T. asiatica roots by the pro- cessing methods of water-washing, water-boiling, water-steaming and stir-frying with wine could all reduce the toddaline content in T. asiatica roots and the reducing effect was especially significant in the stir-frying with wine process. In con- trast, the steaming with wine(the wine-steaming)could increase the toddaline content in T. asiatica. The results of the orthogonal test for the stir-frying with wine process showed that the processing time most greatly influenced the toddaline content, the influence on the content came next from the solid-liquid ratio, and the influence from the frying fire power was lowest. The toddaline content in T. asiatica roots was increased by processing with Wen Huo (the weak frying fire power), with processing time 10 min and solid-liquid ratio 1:1. In contrast, the toddaline content was most significantly reduced when the roots were processed with Zhong Huo(the medium frying fire power), with processing time 30 min and solid-liquid ratio 1:1. Conclusion: The effect of different processing methods on the content of toddaline in T. asiatica was differed in the tested five processing methods. For the stir-frying with wine process, the change of processing parame- ters also caused the different effect on the toddaline content in in T. asiatica.
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AIM To study the anti-inflammatory and analgesic activities of ethanol extract of Toddalia asiatica Lamn.and the mechanism.METHODS Both inflammatory rat model induced by carrageenan and pain model induced by formalin were applied to investigating the analgesic effect of extract of Toddalia asiatica Lam.ELISA kit was used to detect the contents of β-EP,5-HT and PGE2 in serum of carrageenan-treated rats,contents of TNF-αand IL-1β in skin tissue of inflammatory rats,and content of LTB4 in serum of formalin-treated rats;immunohistochemical method was used to observe the SP and FOS protein expressions in rat spinal cord.RESULTS The ethanol extract of Toddalia asiatica Lam.could significantly reduce the rate of toe swelling.In the formalin test,the ethanol extract of Toddalia asiatica Lam.reduced not only the total licking time,but also the content of PGE2,especially in the high dose group.And lowered serum 5-HT contents were observed in all the three dose groups,but a much better performance was found in both the high and low dose groups,and the high dose group's capability in increasing serum β-EP content was also noticed.TNF-α and IL-1β contents in skin tissue were reduced in various dose groups.Middle and high dose groups inhibited FOS protein expression.And the content of LTB4 in serum was obviously decreased in the high dose group.CONCLUSION The anti-inflammatory and analgesic activities of ethanol extract of Toddalia asiatica Lam.may associate with its power in increasing β-EP in serum,decreasing PGE2,5-HT,LTB4 contents,reducing TNF-α,IL-1β contents in skin tissue,and lowering SP and FOS protein expressions in spinal cord.
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Objective:To preliminarily study on the procoagulant activity of Toddalia asiatica Lam. Methods:The plasma recal-cification time affected by the water decoction extract, ultrasonic extract and organic solvent extract from T. asiatica were determined to observe the effects of different extracts from T. asiatica on plasma recalcification time of rabbits. Results:The ultrasonic extract of T. asiatica could reduce the plasma recalcification time (P<0. 05), the ethyl acetate and methanol extract could significantly reduce the plasma recalcification time (P <0. 01), while the water decoction extract prolonged the plasma recalcification time (0.01