RESUMEN
OBJECTIVE:To establish a method for the content determination of vitamin B1 and vitamin B2 in Trivitamin and calci-um gluconate,calcium hydrogen phosphate chewable tablets. METHODS:HPLC was conducted. The column was Inertsil C18 with the mobile phase of 1%triethylamine contained 0.02 mol/L of sodium heptane solution(pH was adjusted to 5.5 by phosphate)-methanol (74∶26,V/V)at the flow rate of 1.0 ml/min,the detection wavelength was 260 nm,temperature was 30℃and the volume was 20μl. RESULTS:There was a good linear relationship between the quality concentration of vitamin B1 and peak area in the range of 0.22-1.10μg/ml(r=0.999 8)and vitamin B2 was in the range of 0.22-1.09μg/ml(r=0.999 7). The RSDs of precision,reproducibility and stabil-ity tests were all less than 1%;the average recovery was respectively 99.66%(RSD=0.96%,n=9)and 100.31%(RSD=0.92%,n=9). CONCLUSIONS:The method is simple,accurate,sensitive and reliable and suitable for the content determination of vitamin B1 and vitamin B2 in Trivitamin and calcium gluconate,calcium hydrogen phosphate chewable tablets.