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Objective: To observe the mechanism of Alpinia (A.) oxyphylla in the treatment of diabetic nephropathy by regulating blood glucose level. Methods: A total of 40 db/db diabetic nephropathy mice were randomly divided into four groups including model group, irbesartan group, A. oxyphylla compound formula high dose group and A. oxyphylla compound formula low dose group with 10 in each group. Ten normal mice served as normal control group. Normal group and model group were given normal saline only. Irbesartan group and A. oxyphylla compound formula high and low dose group were given corresponding drugs once a day for 4 weeks (about 0.2 mL, once a day). Blood glucose, BUN, urine protein, SCr excretion, GSH and CAT activity were detected. Results: Compared with normal group, the blood glucose of model group were increased significantly (P<0.05). While compared with model group, the blood glucose of irbesartan group and A. oxyphylla compound formula groups (both high and low dose) decreased significantly after 4 weeks' treatment. Compared with model group, 24 h urine protein, BUN and SCr of A. oxyphylla compound formula groups (both high and low dose) decreased significantly(P<0.05) after 4 weeks' treatment. Compared with normal group, GSH, CAT of model group decreased significantly (P<0.05). While compared with model group, GSH, CAT of irbesartan group and A. oxyphylla compound formula high dose group increased significantly (P<0.05). Conclusions: A. oxyphylla compound formula can protect diabetic nephropathy mice by reducing the blood glucose level, decreasing the excretion of urine protein, BUN, SCr, and increasing the activites of CAT and GSH.
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Objective: To observe the neurovascular mechanism of Rattan Moxibustion in the treatment of blood stasis type diabetic peripheral neuropathy . Methods: Seventy db/db mice were randomly divided into 7 groups including normal group, blood stasis model group, α-lipoic acid group, fake Rattan Moxibustion group, and three subgroups of Rattan Moxibustion-15-min, 20-min, and 25-min group respectively. After 6 weeks' Rattan Moxibustion therapy, thermal pain threshold, blood glucose, endothelin, nitric oxide, nitric oxide synthase and malondialdehyde levels were detected, and pancreatic tissues morphology were observed under light microscopy. Heme oxygenase 1 (HO-1) proteins in dorsal root neuron were detected by Western blotting. Results: Compared with normal group, blood stasis model group, fake Rattan Moxibustion group, thermal pain threshold of Rattan Moxibustion group was significantly improved (P<0.05); the blood glucose, endothelin, nitric oxide, nitric oxide synthase and malondialdehyde levels of the 20-min group were significantly ameliorated (P<0.01). In the Rattan Moxibustion groups, the chronic inflammation was found to be reduced according to hematoxylin-eosin staining results and Rattan Moxibustion groups HO-1 expression has been strengthened. Conclusions: Rattan Moxibustion therapy can significantly improve peripheral nerve function in diabetic peripheral neuropathy of blood stasis type in mice, improve the blood stasis of pathological state; it can also reduce the chronic inflammation of the pancreas. The mechanism may ameliorate vasoactive and antioxidant factors, enhance HO-1 expression, ultimately improve microcirculation and enhance antioxidant capacity of the model mice.
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Objective: To explore the clinical effects of tropical Eucommia ulmoides compound-Rattan Moxibustion liquid external rubbing therapy which is from medicine of Li Nationality in Hainan combined with SET sling exercise therapy technique and lumbar-abdominal muscle training program on treating nonspecific low back pain (NLBP) as well as its influence on serum 5-hydroxytryptamine and beta-endorphin levels. Methods: From June 2016 to June 2018, 42 NLBP patients were admitted to the outpatient and ward of Rehabilitation Department of the First Affiliated Hospital of Hainan Medical College, who were randomly divided into control group (n=21) and experimental group (n=21). On the basis of traditional massage manipulation, the control group adopts lumbar and abdominal muscles training, while Rattan Moxibustion liquid external rubbing therapy combined with SET sling exercise therapy was used in the experimental group. The treatment was given once every other day, three times a week and 20 min each time for 8 weeks. Then ODI index and VAS scale of low back pain, as well as serum 5-HT and beta-EP levels were observed and detected. Results: The improvement in low back pain index ODI index of the experimental group were better than that of the control group (control group from 21.23±1.57 to 4.78±0.86 while experiment group from 21.27±1.36 to 2.75±0.64 after 8 weeks' treatment)(P<0.05). VAS scale decreased significantly in the experiment group compared with control group (control group from 6.62±1.51 to 2.71±0.92 while experiment group from 6.71±1.55 to 1.83±0.79 after 8 weeks' treatment)(P<0.05). Serum 5-HT in the experimental group were significantly improved compared with those in the control group [control group from (70.15±20.64) ng/mL to (127.34±40.59) ng/mL while experiment group from (68.72±19.18) ng/mL to (142.65±38.72) ng/mL after treatment](P<0.05) and beta-EP level ameliorates compared with control group [control group from (180.63±51.87) pg/mL to (225.76±57.63) pg/mL while experiment group from (172.39±50.72) pg/mL to (251.26±62.89) pg/mL after treatment](P<0.05). Conclusions: Tropical herb-Rattan Moxibustion liquid external rubbing therapy combined with SET sling exercise therapy technology shows significant effects on NLBP. The mechanism may be related to the improved serum 5-HT and beta-EP levels.