RESUMEN
OBJECTIVE: To study the effects of ultrafine grinding technology on the dissolution of triterpenoids from Ganoderma lucidum.METHODS: UV spectrophotometry was used to determine total extration rates of triterpenoids from G. lucidum. HPLC method was used to determine the contents of 9 kinds of triterpenoids (ganoderic acid A, ganoderic acid B, ganoderic acid C, ganoderic acid C1, ganoderic acid C2, ganoderic acid D, ganoderic acid E, ganoderic acid G, ganoderic acid H) from G. lucidum. The total extration rates and the contents of 9 kinds of triterpenoids were compared between 30, 50, 80 mesh common G. lucidum powder and 300 mesh G. lucidum ultramicro powder. RESULTS: The total extraction rates of triterpenoids from 30, 50, 80, 300 mesh G. lucidum powder were (0. 74 ± 0. 08)％,(0. 75 ± 0. 06)％,(0. 78 ± 0. 06)％,(1. 09 ± 0. 10)％ (RSD< 2％, n=3), respectively. With the increase of the mesh number of G. lucidum powder, the contents of triterpenoids were increased gradually, and were the highest in 300 mesh, which was significant higher than common G. lucidum powder (P<0. 05). CONCLUSIONS: After ultrafine grinding, the dissolution of triterpenoids from G. lucidum is increased.
RESUMEN
Objective: To compare the differences in the dissolution behavior of puerarin, paeoniflorin, and liquiritin between ultrafine powder and fine powder of Shenzhu Yichang Pills to provide experimental evidence for optimize the production technology. Methods: The relative cumulative dissolution rates of powders of Shenzhu Yichang Pills in different dissolution mediums were quantitatively studied by HPLC column of Agilent Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm), mobile phase of CH3CN-0.1% H3PO4 (1189), flow rate of 1.5 mL/min, detection wavelength of 230 nm, and the volume of injection was 10 μL. Results: The relative cumulative dissolution rates of puerarin and liquiritin in distilled water and artificial gastric fluid for fine powder were bigger than that for ultrafine powder, but the relative cumulative dissolution rates of paeoniflorin in distilled water and artificial gastric fluid for fine powder were smaller than that for ultrafine powder. The dissolution behavior of fine powder was similar to that of ultrafine powder in artificial intestinal fluid. Conclusion: An appropriate degree of ultrafine grinding can promote the dissolution of active ingredients of Shenzhu Yichang Pills, and the dissolution is influenced by the dissolution medium. Further research is needed to the application of ultrafine grinding technology for Shenzhu Yichang Pills.