Relationship between Umbilical Artery Blood Flow, Levels of sEng and NO and Perinatal Outcome in Patients with Preeclampsia / 天津医药

Objective: To explore the association of fetal umbilical artery (UA) Doppler blood flow parameters with umbilical vein (UV) serum sEng,nitric oxide (NO) level and the perinatal outcome in patients with preeclampsia(PE). Methods: Color ultrasonography was used to detect the resistive index (RI), pulse index (PI), the peak systolic velocity (Vs), diastolic velocity (Vd) and the peak-systolic/diastolic ratio(S/D) of UA in 39 PE patients(20 cases of mild preeclampsia, 19 cases of severe preeclampsia), 20 cases of normal full-term pregnant women and 20 gestational hypertension(GH) women. The UV serum level of sEng was analyzed using enzyme-linked immunosorbent assay (ELISA). The level of NO was determined using nitric acid recovery, and to analysis of perinatal outcome. Results: Compared with normal pregnancy and GH, levels of S/D, RI, PI and sEng were significantly higher in the mild and severe preeclampsia group(P < 0.05), and levels of Vs, Vd and NO were significantly lower(P < 0.05). There was a negative correlation between the values of PI, RI and S/D with the level of UV serum NO in the mild and severe preeclampsia group(P < 0.05). There was a positive correlation between the values of PI, RI and S/D with the level of UV serum sEng in the mild and severe PE group (P < 0.05). The incidence of perinatal disease was significantly higher in PE (P < 0.05). Compared with normal perinatal infants, the abnormal perinatal infants had obviously higher levels of S/D, RI, PI and sEng (P < 0.05), and lower levels of Vs, Vd and NO(P < 0.05) in PE. Conclusion: The umbilical artery blood flow parameters can reflect the disease condition and fetal status, which has an important clinical value.

The Histochemical Localization and Activities of Nitric Oxide Synthase in Placental Tissue from Pregnancies with Abnormal Umbilical Artery Flow Velocity Waveforms / 中华围产医学杂志

Objective To study the nitric oxide synthase(NOS)distribution and activity in placental villous,stem villous vessel and umbilical vessel from pregnancies with abnormal umbilical artery flow velocity waveforms and discuss the effect of NOS in the development of abnormal umbili- cal artery flow velocity waveforms.Methods Using NADPH-diaphorase histochemical method and colorimetry,we studied the distribution and activity of NOS in placental villous,stem villous vessel and umbilical vessel tissues from nine women with normal umbilical artery Doppler waveforms and eight women with abnormal umbilical artery Doppler waveforms.Results The NOS primarily dis- tributed in the syncytiotrophoblast and vessel endothelial cells.In the majority syncytiotrophoblast of normal group,the blue granules gathered to mass,appeared primarily basal in location.In the ma- jority syneytiotrophoblast of abnormal group,the blue granules diffused primarily around nucleus, the staining intension was weaker than normal.Placental villous from the abnormal group had signif- icantly lower activities of NOS than the normal group.Conclusions The lower activities and an abnormal distribution of NOS were found in the placental villous of women with abnormal umbilical artery flow velocity waveforms.The abnormity of NOS may contribute to the development abnormal umbilical artery flow velocity waveforms.