Active constituents of Urtica fissa in inhibition of benign prostatic hyperplasia / 中国中药杂志

The present study investigated the material basis of Urtica fissa for the inhibition of benign prostatic hyperplasia(BPH). The active fractions were screened, and the extracts of dichloromethane and ethyl acetate exhibited significantly inhibitory activities against 5α-reductase in vitro and BPH in model rats. The chemical constituents in the active fractions were systematically investigated, and 28 compounds were obtained, which were identified as lobechine methyl ester(1), dibutyl-O-phthalate(2), 1-monolinolein(3), epipinoresinol(4), 5-hydroxy-3,4-dimethyl-5-pentanyl-2(5H)-furanone(5), E-7,9-diene-11-methenyl palmitic acid(6), evofolin B(7), ficusal(8), threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-ethoxypropan-1-ol(9), α-viniferin(10),(9R,7E)-9-hydroxy-5,7-mengatigmadien-4-one-9-O-β-D-glucopyranoside(11), indole-3-carboxaldehyde(12), p-hydroxy ethyl cinnamate(13), benzyl alcohol-O-β-D-glucoside(14), L-methionine(15), 4-methoxyaniline(16), 6-aminopurine(17), 8'-acetyl oilvil(18), 4-methoxyl-8'-acetyl oilvil(19), vanillic acid(20), β-hydroxypropiovanillone(21), 7-hydroxy-6-methoxycoumarin(22), p-hydroxybenzaldehyde(23), pinoresinol(24), erythro-1,2-bis-(4-hydroxy-3-methoxyphenyl)-1,3-propanediol(25), urticol(26), urticol-7-O-β-D-glucopyranoside(27), and lobechine(28). Compounds 1-17 were isolated from U. fissa for the first time. Meanwhile, compound 1 was a new natural product. Compounds 10, 11, 19, 21, and 27 exhibited significant inhibitory effects on 5α-reductase.

Liposoluble Constituents of Urticae Rhizoma / 中国药学杂志

OBJECTIVE: To investigate the liposoluble constituents of Urticae Rhizoma. METHODS: The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS gel column chromatographies, and semi-preparative HPLC. The structures were elucidated on the basis of spectral data and physiochemical properties. RESULTS: Twenty-one compounds were isolated from the ethyl acetate fraction of Urticae Rhizoma, and identified as(-)-urticol(1),(-)-secoisolariciresinol(2), 23-hydroxybetulinic acid(3), 2α,3β, 24-trihydroxy-12-oleanen-28-oic acid(4), cleomiscosin A (5), dihydro-4-hydroxy-5-hydroxymethyl-2(3H)-furanone(6), methyl chlorogenate(7), kaempferol(8), pinoresinol monomethyether-4'-O-β-D-glucopyranoside(9), martairesinol-4'-O-β-D-glucopyranoside(10), cycloolivil-6-O-β-D-glucopyranoside(11), stigmasterol-3-O-β-D-glucopyranoside(12), nicotinamide(13), trans-caffeic acid-4-O-β-D-glucopyranoside(14), esculin(15), 5-hydroxyl-7-methoxycoumarin-8-O-β-D-glucopyranoside(16), 6-oxymethyluteolin-7-O-β-D-glucopyranoside(17), luteolin-7-O-β-D-glucopyranoside(18), quercetin-3-O-(4″-methoxy)-α-L-rhamnopyranoside(19), 2'-deoxy uridine(20), and apigenin-6, 8-di-C-β-D-glycoside(21), respectively. CONCLUSION: All the compounds, except 8 and 12, are isolated from U. fissa for the first time. Meanwhile, compounds 5, 6, 9, 10, 11, 14, 16, 17, and 19 are all found in Urticaeae plants for the first time.

Chemical constituents of antirheumatism fraction from Urtica fissa / 中草药

Objective To study the chemical constituents of the EtOAC soluble fraction from Urtica fissa with obviously anti-inflammatory and analygestic effect. Methods The compounds were isolated by polyamide and silical gel colum chromatography and identified by UV, IR, NMR, and MS. Results Twelve compounds were isolated from U. fissa and identined as quercetin (Ⅰ), kaempferol (Ⅱ), kaempferol-3-O-?-D-glucopyranoside (Ⅲ), quercetin-3-O-?-D-glucopyranoside (Ⅳ), caffeic acid (Ⅴ), chlorogenic acid (Ⅵ), scopolin (Ⅶ), scopoletin (Ⅷ), kaempferol-3-O-rutinoside (Ⅸ), quercetin-7-O-?-D-glucopyranoside (Ⅹ), isorhamnetin-3-O-?-D-rutinoside (Ⅺ), ?-sitosterol ( ⅩⅡ ). Conclusion Compounds Ⅰ-Ⅺ are all isolated from U. fissa for the first time and compounds Ⅶ, Ⅹ, and Ⅺ are found in plants of Urtica L. for the first time also.