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1.
Journal of Veterinary Science ; : 67-70, 2002.
Artículo en Inglés | WPRIM | ID: wpr-172834

RESUMEN

This experiment was conducted to assess the efficacy of typhoid vaccine newly produced by purifying Vi antigen of Salmonella typhi. With Karber method, LD50 of challenging organism (S. typhi ty2) was determined as 6.31 CFU/mouse, and then the organism was used for the study. With Probits method, ED50 of the vaccine was determined as 0.016 microgram / 0.5 ml / mouse. The ELISA titer (0.5097+/-0.0606) was 4 times in the group treated with high dose (0.25 microgram/0.5ml) as in control (0.1113+/-0.0110). Six major protein bands of 66, 55, 35, 33, 18, and 9 kd were detected in Western blot analysis with serum of a vaccine treated mouse, whereas only one weak band of about 35 kd was detected with serum of a control mouse. We concluded that typhoid vaccine produced by purifying Vi antigen of S. typhi very effectively prevent S. typhi infection in mice.


Asunto(s)
Animales , Masculino , Ratones , Anticuerpos Antibacterianos/inmunología , Antígenos Bacterianos/inmunología , Western Blotting , Ensayo de Inmunoadsorción Enzimática , Dosificación Letal Mediana , Modelos Logísticos , Ratones Endogámicos BALB C , Polisacáridos Bacterianos/inmunología , Salmonella typhi/química , Fiebre Tifoidea/inmunología , Vacunas Tifoides-Paratifoides/administración & dosificación
2.
Korean Journal of Infectious Diseases ; : 379-384, 1998.
Artículo en Coreano | WPRIM | ID: wpr-21695

RESUMEN

BACKGROUND: Typhoid fever is diagnosed by culture or serological study. The confirmative diagnosis of typhoid fever is made by culture of the causative orga-nism usually from body fluids. Serological test is a supportive diagnostic tool, which is useful for early dia-gnosis. In Severance Hospital, Vi-indirect fluorescent antibody test(Vi-IFAT) using the Vi-antigen of Salmo-nella typhi has been used in the diagnosis of typhoid fever since 1989. We investigated the test results from the past 7 years, in order to clarify the sensitivity and specificity of Vi-IFAT. METHODS: A retrospective study was done on pa-tients whose chief complaint was fever and who were tested using Vi-IFAT in the Severance Hospital from 1989 to 1996. The positive value for Vi-IFAT was de- fined as 1:64 or higher. RESULTS: The sensitivity and specificity of Vi-IFAT for typhoid fever was 94.4% and 95.1%, respectively. The positive and negative predictive values were 85.7% and 98.2% respectively. Positive rates of Vi-IFAT after fever onset increased with time and 68% were positive before the first week. From the first to the second week, 89.5% were positive and after the second week, 100% were positive. CONCLUSION: Vi-IFAT is not only a valuable sero-logic test for the diagnosis of typhoid fever, but also useful in the early diagnosis of the disease.


Asunto(s)
Líquidos Corporales , Diagnóstico , Diagnóstico Precoz , Fiebre , Estudios Retrospectivos , Sensibilidad y Especificidad , Pruebas Serológicas , Fiebre Tifoidea
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