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Viscum coloratum (Kom.) Nakai is a well-known medicinal plant. However, the optimal harvest time for V. coloratum is unknown. Few studies were performed to analyze compound variation during storage and to improve post-harvest quality control. Our study aimed to comprehensively evaluate the quality of V. coloratum in different growth stages, and determine the dynamic variation of metabolites. Ultra-performance liquid chromatography tandem mass spectrometry was used to quantify 29 compounds in V. coloratum harvested in six growth periods, and the associated biosynthetic pathways were explored. The accumulation of different types of compounds were analyzed based on their synthesis pathways. Grey relational analysis was used to evaluate the quality of V. coloratum across different months. The compound variation during storage was analyzed by a high-temperature high-humidity accelerated test. The results showed that the quality of V. coloratum was the hightest in March, followed by November, and became the lowest in July. During storage, compounds in downstream steps of the biosynthesis pathway were first degraded to produce the upstream compounds and some low-molecular-weight organic acids, leading to an increase followed by a decrease in the content of some compounds, and resulted in a large gap during the degradation time course among different compounds. Due to the rapid rate and large degree of degradation, five compounds were tentatively designated as "early warning components" for quality control. This report provides reference for better understanding the biosynthesis and degradation of metabolites in V. coloratum and lays a theoretical foundation for rational application of V. coloratum and better quality control of V. coloratum during storage.
Asunto(s)
Viscum/química , Plantas Medicinales/química , Cromatografía Liquida , Espectrometría de Masas , MetabolómicaRESUMEN
Viscum coloratum(Komn.)Nakai is a well-known medicinal hemiparasite widely distributed in Asia.The synthesis and accumulation of its metabolites are affected by both environmental factors and the host plants,while the latter of which is usually overlooked.The purpose of this study was to comprehensively evaluate the effects of host and habitat on the metabolites in V.coloratum through multiple chemical and biological approaches.The metabolite profile of V.coloratum harvested from three different host plants in two habitats were determined by multiple chemical methods including high-performance liquid chromatography-ultraviolet(HPLC-UV),gas chromatography-flame ionization detector(GC-FID)and ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-QTOF/MS).The differences in antioxidant efficacy of V.coloratum were determined based on multiple in vitro models.The multivariate statistical analysis and data fusion strategy were applied to analyze the dif-ferences in metabolite profile and antioxidant activity of V.coloratum.Results indicated that the metabolite profile obtained by various chemical approaches was simultaneously affected by host and environment factors,and the environment plays a key role.Meanwhile,three main differential metab-olites between two environment groups were identified.The results of antioxidant assay indicated that the environment has greater effects on the biological activity of V.coloratum than the host.Therefore,we conclude that the integration of various chemical and biological approaches combined with multivariate statistical and data fusion analysis,which can determine the influences of host plant and habitat on the metabolites,is a powerful strategy to control the quality of semi-parasitic herbal medicine.
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Objective: To study the chemical constituents from the dry stem leaves parts of Viscum coloratum. Methods: The chemical constituents were separated and purified by various column chromatographies and HPLC. Their structures were determined on the basis of spectroscopic analyses (1H-NMR, 13C-NMR, 2D-NMR, and MS). The dry stem leaves of V. coloratum were extracted by 95% ethanol, then concentrated and was extracted by water and chloroform. Then the water part was eluted by the gradient of water and ethanol (100:0→0:100), obtaining the six parts of water, 10% ethanol, 30% ethanol, 50% ethanol, 70% ethanol, and 95% ethanol. Results: Nine compounds were isolated from the 50% EtOH extract in the dry stem leaves parts of V. coloratum with the structures identified as rhamnazin-3-O-β-D-api (1→2)-O-β-D-glucoside (1), rhamnazine (2), rhamnazin-3-O-β-D-glucoside (3), rhamnazin-3- O-β-D-(6″-acetyl)-O-β-D-glucoside (4), homeriodictyol-7-O-β-D-glucoside (5), homeriodictyol-7-O-β-D-api (1→2)-O-β-D-glucoside (6), homeriodictyol-7-O-β-D- glucoside-4'-O-β-D-apioside (7), homeriodictyol-7-O-β-D-glucosid (8), and liquidamboside (9). Conclusion: Compound 1 is a new flavonoid glycoside, named as viscumneoside IX, and the compound 9 is isolated from the dry stem leaves parts of V. coloratumi first time.
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A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-O-β-D-apiosiyl-（1→2）-β-D-glucoside was used as a reference. The relative areas of common peaks were used for hierarchical clustering analysis and similarity calculation. Thirty-seven samples collected from different sources were classified into five groups. The similarities of 21 batches Viscum coloratura samples were beyond 0.90. The results obtained suggest that the chromatographic fingerprint can efficiently identify Viscum coloratum. Additionally, the fingerprints can then be used to evaluate the correlation between Viscum coloratura and hosts.
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A high-performance liquid chromatography coupled ultraviolet (HPLC-UV) method was developed for a chemical fingerprint analysis ofViscum coloratum. Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-O-β-D-apiosiyl-(1→2)-β-D-glucoside was used as a reference.The relative areas of common peaks were used for hierarchical clustering analysis and similarity calculation.Thirty-seven samples collected from different sources were classified imo five groups.The similarities of 21 batches Viscum coloratuma samples were beyond 0.90.The results obtained suggest that the chromatographic fingerprint can efficiently identify Viscum coloratum.Additionally,the fingerprints can then be used to evaluate the correlation between Viscum coloratum and hosts.
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Object To determine the activities of various antioxidant enzymes, including superoxide dismutase, catalase, peroxidase and glutathione peroxidase, and the contents of some simpler antioxidants such as glutathione, chlorogenic acid and certain flavonoids in the leaves, stems and fruits of Viscum coloratum (Kom ) Nakai Methods The activities were determined as described in various literature reports Results The plant was found to be rich in various antioxidants Conclusion V coloratum, with its abundant antioxidant constituents, is proved to be a strong antioxidation drug for medical use especially its leaves