Chemical Investigation on an Endophytic fungus Gibberella moniliformis JS1055 Derived from a Halophyte Vitex rotundifolia

Chemical investigation of the ethyl acetate extract of Gibberella moniliformis JS1055 endophytic fungus derived from a halophyte, Vitex rotundifolia, led to the isolation of nine compounds including 7-butyl-6,8-dihydroxy-3(R)-pent-11-enylisochroman-1-one (1), 7-butyl-6,8-dihydroxy-3(R)-pentylisochroman-1-one (2), 7-butyl-6,8-dihydroxy-3(R)-pentylisochroman-1-one (3), 5α,8α-epidioxyergosta-6,9(11),22-trien-3-ol (4), ergosterol peroxide (5), tetradecanoic acid (6), 8-O-methylfusarubin (7), nicotinic acid (8) and adenosine (9). They were identified by extensive spectroscopic data analysis including 1D, 2D (¹H-¹H COSY, HSQC, HMBC) NMR, and ESIMS. All the isolates (1

Chemical compositions and anti-inflammatory activities of essential oils from Aster spathulifolius and Vitex rotundifolia maxim.

The essential oils were prepared from Aster spathulifolius (ASE) and Vitex rotundifolia (VRE) by hydrodistillation and their chemical compositions were investigated by GC–MS. Analysis of ASE provided 15 components and eight of which were identified sesquiterpene compounds. The major components in ASE included germacrene D (35.1 %), trans-caryophyllene (15.9 %) and trans-phytol (14.9 %). On the other hand, VRE provided manoyl oxide (14.3%), α-terpineol (13.1 %) and α-pinene (10.0 %) as major ingredients. To assess the anti-inflammatory effects of the essential oils, the production of nitric oxide (NO) and tumor necrosis factor (TNF)-α were monitored using lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. In this test, ASE and VRE were appeared to suppress both NO and TNF-α synthesis in dose-dependent manner. The results indicate that VRE and ASE could be useful in cosmetic applications as natural products possessing antiinflammatory efficacy