RESUMEN
Objective:To investigate the expressions of O-GlcNAc transferase (OGT) in the hepatocellular carcinoma (HCC) tissue,cells and serum,and to clarify the significance of OGT in diagnosis and treatment of HCC.Methods:Western blotting method was used to detect the expression levels of OGT in 20 samples of HCC tissue and matched non-tumor tissue,8 hepatoma cell lines and normal liver (human) cell lysate.The expression levels of serum OGT were detected by direct ELISA in 20 liver cirrhosis (LC) patients,20 HCC patients and 20 healthy volunteers.The relationship between the expression levels of protein in HCC tissue,cells and serum and the occurrence and development of tumor were analyzed.Results:The Western blotting results showed there were expressions of OGT in both HCC tissue and matched non-tumor tissue;The positive expression rate of OGT in HCC tissue was significantly higher than that in non-tumor tissue (P＜0.05).There were expressions of OGT in both hepatoma cell lines and normal liver (human) cell lysate;the positive expression rate of OGT in HCC cell lysate was significantly higher than that in the normal liver cell lysate (P＜0.05).The result of direct ELISA showed that the serum OGT levels of the LC and HCC patients were equally higher than that of the healthy volunteers (P＜0.05).Conclusion:OGT highly expresses in HCC tissue and OGT is a promising biomarker for the diagnosis of HCC.
RESUMEN
Objective:To investigate the anti-fatigue phenomenon induced by forced swimming in the mice,and to explore the anti-fatigue effect of argininyl fructoyl glucose (AFG) from red ginseng in the mice and its mechanism.Methods:The AFG was extracted from red ginseng.The ICR mice were divided into blank control group,low dose of AFG group (100 mg · kg-1),middle dose of AFG group (200 mg · kg-1) and high dose of AFG group (400 mg · kg-1) (n=20).The mice mere given a forced swimming test after continuous gavage for 28 d.The weights,organ indexes,time of forced swimming,contents of lactic acid (LD),blood urea nitrogen (BUN),hepatic glycogen (Gly) and expressing levels of PGC-1α in gastrocnemius of the mice in various groups were detected.Results:Compared with blank control group,the weights and organ indexes of the mice in low,middle and high doses of AFG groups had no significant differences (P＞ 0.05).Compared with blank control group,the time of forced swimming,contents of Gly and expressing levels of PGC-1α of the mice in low,middle and high doses of AFG groups were significantly increased (P＜0.05 or P＜0.01) in a dose-dependent manner.Compared with blank control group,The contents of LD and BUN in serum of the mice in low,middle and high doses of AFG groups were significantly decreased (P＜0.01).Conclusion:AFG has anti-fatigue effect in mice,and its mechanism may be related to energy metabolism.
RESUMEN
Objective:To investigate the expressions of O-GlcNAc transferase (OGT) in the hepatocellular carcinoma (HCC) tissue,cells and serum,and to clarify the significance of OGT in diagnosis and treatment of HCC.Methods:Western blotting method was used to detect the expression levels of OGT in 20 samples of HCC tissue and matched non-tumor tissue,8 hepatoma cell lines and normal liver (human) cell lysate.The expression levels of serum OGT were detected by direct ELISA in 20 liver cirrhosis (LC) patients,20 HCC patients and 20 healthy volunteers.The relationship between the expression levels of protein in HCC tissue,cells and serum and the occurrence and development of tumor were analyzed.Results:The Western blotting results showed there were expressions of OGT in both HCC tissue and matched non-tumor tissue;The positive expression rate of OGT in HCC tissue was significantly higher than that in non-tumor tissue (P＜0.05).There were expressions of OGT in both hepatoma cell lines and normal liver (human) cell lysate;the positive expression rate of OGT in HCC cell lysate was significantly higher than that in the normal liver cell lysate (P＜0.05).The result of direct ELISA showed that the serum OGT levels of the LC and HCC patients were equally higher than that of the healthy volunteers (P＜0.05).Conclusion:OGT highly expresses in HCC tissue and OGT is a promising biomarker for the diagnosis of HCC.
RESUMEN
Objective:To investigate the anti-fatigue phenomenon induced by forced swimming in the mice,and to explore the anti-fatigue effect of argininyl fructoyl glucose (AFG) from red ginseng in the mice and its mechanism.Methods:The AFG was extracted from red ginseng.The ICR mice were divided into blank control group,low dose of AFG group (100 mg · kg-1),middle dose of AFG group (200 mg · kg-1) and high dose of AFG group (400 mg · kg-1) (n=20).The mice mere given a forced swimming test after continuous gavage for 28 d.The weights,organ indexes,time of forced swimming,contents of lactic acid (LD),blood urea nitrogen (BUN),hepatic glycogen (Gly) and expressing levels of PGC-1α in gastrocnemius of the mice in various groups were detected.Results:Compared with blank control group,the weights and organ indexes of the mice in low,middle and high doses of AFG groups had no significant differences (P＞ 0.05).Compared with blank control group,the time of forced swimming,contents of Gly and expressing levels of PGC-1α of the mice in low,middle and high doses of AFG groups were significantly increased (P＜0.05 or P＜0.01) in a dose-dependent manner.Compared with blank control group,The contents of LD and BUN in serum of the mice in low,middle and high doses of AFG groups were significantly decreased (P＜0.01).Conclusion:AFG has anti-fatigue effect in mice,and its mechanism may be related to energy metabolism.
RESUMEN
Objective To observe the lipid regulation role of electroacupuncture intervention in the rats with non-alcoholic fatty liver disease(NAFLD)induced with high fat and cholesteol forage,and to clarify the regulation mechanism of serum retinol binding protein 4 (RBP4 )level and liver X receptor a (LXR-α)and sterol regulatory element binding protein-1c(SREBP-1c)expressions.Methods 44 female SD rats were fed for 7 d to adapt the environment and were randomly divided into normal group, model group, Dongbaogantai group, electroacupuncture group;11 rats in each group.The rats in normal group got routine feeding,and the others were fed with high fat and high cholesterol forage. After 8 weeks, the models were established, the rats in electroacupuncture group were treated with electroacupuncture method (1.5-2.0 Hz, D.-D.wave, 9V, 1-3 mA)in“Ganshu”,“Pishu”,“Geshu”for 15 min,once a day,lasted for 28 d.The changes of fasting blood-glucose(FBG),serum free fatty acids(FFA)and liver tissue homogenate triglyceride(TG)and total cholesterol (TC)levels of the rats in various groups were tested, and enzyme-linked immunosorbent (ELISA)was used to determine the serum RBP4 levels, and Western blotting method was used to detect the LXR-αand SREBP-1 c protein expression levels in rat liver tissue.Results Compared with normal group,the FBG,serum FFA,TG and TC levels in liver tissue homogenate and serum RBP4 level of the rats in model group were increased (P<0.01);the LXR-αand SREBP-1c protein expression levels were also increased (P<0.01).Compared with model group, the FBG,serum FFA,the TG and TC levels in liver tissue homogenate and serum RBP4 levels of the rats in electroacupuncture group and Dongbaogantai group were decreased (P<0.01);the LXR-αand SREBP-1c protein expression levels were also decreased (P< 0.05 or P< 0.01 ). Conclusion Electroacupuncture method in“Fenglong”,“Zusanli”,“Sanyinjiao”can reduce the serum RBP4 level, regulate the lipid metabolism, and improve the lipid deposition of the NAFLD rats;they have obvious therapeutic effect on NAFLD, and its mechanism may be related to inhibiting the increasing of LXR-αand SREBP-1 c protein expressions in liver tissue.