RESUMEN
Objective:To establish the method for the determination of five anthraquinones constituents including aloe-emodin , rhein, emodin , chrysophanol and physcion in Yanxiaodinaer syrups. Methods: An HPLC method was used with a DIONEX Acclaim 120A C18 (250 mm × 4. 6 mm,5 μm) column. The mobile phase was acetonitrile-0. 1% phosphoric acid with gradient elution at flow rate of 1. 0 ml·min-1 . The detection wavelength was 254nm,the column temperature was 30℃and the injection volume was 10 μl. Results:The linear ranges of the five anthraquinones were 0. 018 7-0. 467 0 μg(r=0. 999 8),0. 020 2-0. 504 0 μg(r=0. 999 9), 0.013 0-0.390 6 μg(r=0.999 9),0.013 3-0.399 9 μg(r=0.999 9)and 0.009 3-0.277 8 μg(r=0.999 9), and the recoveries were 94. 30%(RSD=2. 68%,n=6),95. 49%(RSD=2. 50%,n=6),93. 70%(RSD=2. 42%,n=6),92. 52%(RSD=2. 40%,n=6) and 93. 72%(RSD=2. 54%,n=6), respectively. Conclusion:The method is accurate , sensitive and reproducible, which can be used as the basis for the quality control of Yanxiaodinaer syrups.