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OBJECTIVE@#To investigate the protective effect of Zengye Decoction (, ZYD) on the submandibular glands (SMGs) in nonobese diabetic (NOD) mice.@*METHODS@#Twenty-seven female NOD mice were randomly equally divided into 3 groups: the model group, the hydroxychloroquine (HCQ) group, and the ZYD group. Nine C57/B6 mice served as the normal group. After 1-week acclimation, the HCQ and ZYD groups were intragastrically administered with HCQ and ZYD, respectively, and the normal and model groups were administered with normal saline. Changes in the salivary flow rate were observed. Mice from all 4 groups were sacrificed at the age of 20 weeks. The serum and SMGs were collected. Serum cytokines gamma-interferon (IFN-γ), interleukin-10 (IL-10) were detected by enzyme-linked immunosorbent assay. Histological changes in the submandibular glands were examined by hematoxylin and eosin staining. The mRNA expression of IFN-γ, IL-10 and vasoactive intestinal peptide (VIP) in the submandibular glands were measured by real-time polymerase chain reaction.@*RESULTS@#Compared with the model group, the salivary flow of the ZYD group significantly increased (P<0.05), the extent of the histological changes was ameliorated (P<0.05), and the Th1/Th2 cytokine imbalance was remedied (P<0.05). In the ZYD-treated mice, the VIP mRNA was up-regulated (P<0.05).@*CONCLUSIONS@#ZYD is beneficial in protecting structure and function of SMGs in NOD mice. The mechanism may be associated with the correction of the Th1/Th2 cytokine imbalance, and with the prevention of a progressive decline of the VIP level.
Asunto(s)
Animales , Femenino , Ratones , Citocinas , Sangre , Medicamentos Herbarios Chinos , Farmacología , Ratones Endogámicos C57BL , Ratones Endogámicos NOD , Salivación , Síndrome de Sjögren , Quimioterapia , Alergia e Inmunología , Glándula Submandibular , Patología , Células TH1 , Alergia e Inmunología , Células Th2 , Alergia e Inmunología , Péptido Intestinal Vasoactivo , GenéticaRESUMEN
Objective]To summarize the principle of usage of Chenqi Decoction Family, and compare with the similarities and differences of those in ShangHan Lun. [Method]To classify and analyse the scriptures related to Chenqi Decoction Family, and make a comparison with those in Chapter of Yangming Disease in ShangHan Lun. [Result]Besides the method of purgation and urgent purgation to preserve Yin, Wu Jutong inhertied and developed the purgation method of Zhang Zhongjing’s ShangHan Lun, such as Fluid-increasing Decoction, The New Yellow Dragon Decotion, The Lung-ventilating Chenqi Decotion, The Heart-fire Conduction Chenqi Decotion, The Calculi bovis Chenqi Decotion and The Fluid-increasing Chenqi Decotion, which enriched the syndrome differentiation system of ferbile disease. A deeper comparison of WenBingTiaoBian and ShangHanLun is worth researching.
RESUMEN
Objective To observe clinical effect of treating recurrent oral ulcer with modified Zengye decoction combined with levamisole. Methods 65 patients with recurrent oral ulcer were randomly recurited into a control group and a treatment group. The control group was treated with levamisole, and the treatment group was treated with modified zengye decoction plus levamisole. The therapeutic effects of both groups were observed. Results The therapeutic effect of the treatment group was significantly better than that of the control group (P<0.05). Conclusion The clinical effect of treating recurrent oral ulcer with levamisole combined with modified Zengye decoction has better effect than using levamisole alone.