Content Determination of TancantongⅡA and Cryptotanshinone in Zhiwei Fuwei Wan by RP-HPLC / 中国中医药信息杂志

Objective To establish the method for determining the content of cryptotanshinone and tanshinone ⅡA in Zhiwei Fuwei Wan. Methods The determination of cryptotanshinone and tanshinone ⅡA was performed on Symmetry C18 column with mobile phase consisted of acetonitrile-0.2%phosphate (60∶40) at flow rate of 1.0 mL/min. The detection wavelength was set at 262 nm. Results The linear ranges of cryptotanshinone and tanshinone ⅡA were 0.081 4-0.407 2 μg (r=0.999 7) and 0.163 6-0.81 8 μg (r=0.999 5) respectively, the average recoveries were 99.70% (RSD=1.37%) and 99.22% (RSD=0.94%) respectively. Conclusion The method is simple and rapid for the quality control of the product.