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1.
Journal of Experimental Hematology ; (6): 1475-1480, 2023.
Artículo en Chino | WPRIM | ID: wpr-1009999

RESUMEN

OBJECTIVE@#To investigate the role of multiple serological methods in the identification of complex antibodies.@*METHODS@#The blood group antigens were detected by saline and microcolumn agglutination methods. The saline method was used to screen and identify IgM-type antibodies in the patient's serum, while the polybrene, anti-globulin, microcolumn agglutination, enzymic and absorption-elution methods were used to screen and identify IgG-type antibodies.@*RESULTS@#The patient was B/CCDee/Jk(a-b+)/Fy(a-b+) blood type. The serum reacted with panel cells, and the reaction presented anti-E pattern in the saline medium. It was fully positive in the microcolumn agglutination card, except 2 negative ones after using papain to treat the panel cells. Referring to the pattern table, it was concluded that there existed anti-c, anti-E, and anti-Jka antibodies, and one antibody corresponding to an antigen that was easily destroyed by papain. The red blood cells with specific phenotype were selected for absorption-elution to identify IgG-type anti-c, anti-E, anti-Jka and anti-Fya antibodies.@*CONCLUSION@#It is confirmed that IgM-type anti-E, and IgG-type anti-c, anti-E, anti-Jka and anti-Fya antibodies exist in the patient's serum by multiple serological methods.


Asunto(s)
Humanos , Papaína , Antígenos de Grupos Sanguíneos , Eritrocitos , Inmunoglobulina G , Inmunoglobulina M
2.
Korean Journal of Blood Transfusion ; : 74-79, 2010.
Artículo en Coreano | WPRIM | ID: wpr-200867

RESUMEN

Several approaches have been introduced to detect allo-antibodies in the presence of warm auto-antibodies, and these methods include warm autoadsorption, cysteine-activated papain and dithiothreitol (ZZAP), and polyethylene glycol (PEG) and dilution of the patient's serum. Among them, the dilution technique is a simple and rapid method. During pretransfusion testing of a 33 year-old systemic lupus erythematosus (SLE) patient with warm auto-antibodies, antibody identification was done by the dilution technique with using serum diluted 1-in-8. The patient demonstrated an anti-Fy(b) pattern of reactivity in his sera. Contrary to our expectations, the phenotype of the erythrocytes was Fy(a+/b+) and the genotype, as assessed by performing PCR-restriction fragment length polymorphism (RFLP), was FY*A/FY*B. These results suggest that the antibody is an autoantibody showing anti-Fy(b) specificities. An antibody identification test using undiluted serum showed the same result when 40 days had passed. We report here on a case with auto-anti-Fy(b) proven by the dilution method in the presence of warm autoantibodies.


Asunto(s)
Humanos , Autoanticuerpos , Ditiotreitol , Eritrocitos , Genotipo , Técnicas de Dilución del Indicador , Lupus Eritematoso Sistémico , Papaína , Fenotipo , Polietilenglicoles , Sensibilidad y Especificidad
3.
The Korean Journal of Laboratory Medicine ; : 57-59, 2003.
Artículo en Coreano | WPRIM | ID: wpr-186846

RESUMEN

A 72-year-old man with a regrowing tongue mass and refractory anemia received two units of compatible red blood cells by saline and albumin phase crossmatching tests. One hour after the last unit, he had a hemolytic transfusion reaction manifested by chest discomfort, fever, redcolored urine, fall in hemoglobin, hemoglobinuria and bilirubinemia. He developed acute renal failure and pulmonary edema and died three days later. Anti-E+Fy(a) antibodies were identified in the patient's serum. This case suggests that indirect antiglobulin crossmatching tests or antibody screening tests are needed for routine pretransfusion tests in Korea.


Asunto(s)
Anciano , Humanos , Lesión Renal Aguda , Anemia Refractaria , Anticuerpos , Incompatibilidad de Grupos Sanguíneos , Eritrocitos , Fiebre , Hemoglobinuria , Hiperbilirrubinemia , Corea (Geográfico) , Tamizaje Masivo , Edema Pulmonar , Tórax , Lengua
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