Expression profile analysis of long non-coding RNAs in hippocampus of alcohol-dependent mice induced by double-bottle selective drinking / 中国生物制品学杂志

@#Objective To analyze the expression profiles of long non-coding RNAs(lncRNA)in hippocampus of alcoholdependent mice induced by double-bottle selective drinking.Methods The alcohol-dependent mouse model was established by double-bottle selective drinking method,and the control group was set up(drinking water). Three male mice with alcohol preference more than 60% and alcohol consumption more than 10 g/(kg·24 h)in alcohol group and random three male mice in control group were selected,of which bilateral hippocampal brain tissues were isolated and stored in liquid nitrogen. LncRNA and mRNA of mouse hippocampal brain tissue RNA samples were sequenced by using Agilent-084388 microarray,and the differential expression of lncRNA in samples was detected by using ncRNA microarray. The biological processes and signaling pathways involved in differential expression of lncRNA were clustered and enriched by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis. Pearson correlation analysis was used to predict the coding genes co-expressed by each differentially expressed lncRNA. Hypergeometric distribution test was used to calculate the significance of differential gene enrichment in each corresponding transcription factor item,and Cytoscape software was used to draw a visual network diagram.Results Compared with the control group,totally 855 lncRNAs(FC ≥ 2. 0,P < 0. 05)were differentially expressed in the hippocampus of mice in alcohol group,of which 337 lncRNAs were up-regulated significantly,with NONMMUT025786.2 and NONMMUT072246.2 being the most up-regulated,and 518 significant downward adjustments were observed,with the largest downward adjustments being NONMMUT113098.1 and NONMMUT076455.1. There were 361 mRNAs differentially expressed in the two groups(FC ≥ 2. 0,P < 0. 05)with 271 mRNAs up-regulated significantly and 90 significant downward adjustments,among which,the most obvious up-regulated were Upf3b and Zfp943,and Adamts 13 and Ift 27 showed the largest downward adjustments. The differential expression of lncRNAs was most obvious in the positive regulation of cell surface,GTPase activity and cell vesicle transport;The main signaling pathways involved were propanoate metabolism,taurine metabolism,extracellular matrix receptor interaction and AMPK signaling pathway. The most abundant transcription factors were FOXL1 and LHX3,with 25 and 21 corresponding co-expressed genes,respectively.Conclusion Through high-throughput gene expression profile microarray analysis,the possible key regulatory sites of lncRNAs and mRNAs were obtained,which provided experimental basis for research of the molecular mechanism of alcohol dependence in the hippocampus.

Design and application of distal radial artery hemostat / 中华危重病急救医学

Transradial approach is the classical access for coronary angiography and percutaneous coronary intervention (PCI). With the increase in the number of interventional procedures, some disadvantages of the transradial approach have also been found, it is easy to lead to various complications, such as radial artery occlusion, radial nerve injury, and puncture difficulties after radial artery spasm. Therefore, some experts put forward the approach of distal radial artery approach for interventional therapy, which has the advantages of convenient positioning, easy postoperative hemostasis, less damage to the proximal radial artery and improving patients' comfort. However, there is no special distal radial artery hemostat in clinic, which limits the development of this approach to a certain extent. Therefore, based on the principles of anatomy and physics, cardiovascular physician at Jiading District District Central Hospital in Shanghai designed and invented a distal radial artery hemostatic device, which is convenient for clinical hemostasis of distal radial artery puncture, and obtained the National Utility Model Patent (patent number: ZL 2021 2 2097829.6). The hemostatic device consists of a glove body with a silicone gasket protruding towards the skin on the inner surface and a binding component. The patient's hand is inserted into the glove body, and after being fixed by the restraint component, the silicone gasket can effectively compress the location of the radial artery puncture point, and play a good hemostatic effect with less pressure, avoid the common complications of proximal radial artery hemostatic, and reduce the discomfort of the patient. Has good application value.

Psychological dependence liability of gaboxadol in two-bottle free-choice model in mice / 中国药理学与毒理学杂志

OBJECTIVE To evaluate the psychological dependence of the extrasynaptic GABAA receptor(GABAAR)agonist gaboxadol and compare it with the synaptic GABAAR modulator midazolam in the two-bottle free-choice model of mice.METHODS ① Male C57BL/6J mice were ig administered with vehicle,midazolam(59.0,73.7,92.2,115.2,144.0 and 180.0 mg·kg-1)or gaboxadol(8.4,10.5,13.1,16.4,20.5,25.6 and 32.0 mg·kg-1),and the loss of righting reflex was observed.The median effective dose(ED50)was obtained from the dose-response curve.② A two-bottle free-choice model was used to find out whether gaboxadol and midazolam induced preference behavior in mice.The mice were divided into normal control,gaboxadol or midazolam groups.During the habituation stage(the first day to the third day,D1-D3),both test and vehicle bottles contained water.During the trail stage(D4-D5),4%sucrose solution was provided in both bottles.During the test stage(D6-D15),test bottles contained vehicle,gaboxadol(3.9×10-6 mol·L-1)or midazolam(1.4×10-5 mol·L-1)in sucrose solu-tions,while other bottles contained the corresponding vehicle in sucrose solutions.Bottles were placed on the two sides of the home cage,to which mice had free access,and their consumption from each bottle was recorded daily.Total consumption,accumulated daily consumption,relative consumption,and accumulated relative consumption during the test stage were calculated.The weight of the mice was also recorded.RESULTS ① Midazolam and gaboxadol dose-dependently increased the rate of loss of right reflex in mice,with ED50 of 105.3 mg·kg-1(95%CI:96.4-115.2 mg·kg-1,R2=0.9796),13.7 mg·kg-1(95%CI:12.6-15.0 mg·kg-1,R2=0.9773),respectively.② Compared with the normal control group,there was no significant difference in the total consumption in the gaboxadol and midazolam groups.Compared to the vehicle bottles,the daily consumption from test bottles in the midazolam group increased significantly on D11-D15 of the test stage(P<0.05),while daily consumption from gaboxadol test bottles was significantly higher than that of vehicle bottles(P<0.01).Compared with the normal control group,the daily relative consumption in the gaboxadol group was significantly increased on D9(P<0.05),and the accumulative relative consumption was significantly higher than in the normal control group(P<0.01).There was no significant change in body weight across the groups over the test stage.CONCLU-SION Like midazolam,gaboxadol exhibits psychological dependence potential in a two-bottle free-choice model.

Influence of Different Plant Growth Regulators and Boron on Growth, Yield and Sex Expression of Bottle Gourd (Lagenaria siceraria Mol.) under Garhwal Valley

The present investigation was carried out at Horticultural Research Centre, Department of Horticulture, H. N. B. Garhwal University, Uttarakhand, India during summer season, 2018-19. The existing experiment was laid out in randomized block design following 13 treatments replicated thrice. The various growth parameters as well as yield attributes and yield were recorded during the experimentation. The result of the present investigation showed that GA3 @ 50 mg/kg was registered as the best treatment in terms of vine length (376.13 cm), fruits per vine (6.60), sex ratio (F:M) 1:11.07 and yield per vine (1357.33 g) and NAA @ 200 mg/kg+ Boron @ 0.1% with relation to the number of main branches per vine (10.87), length of fruit (24.57 cm), diameter of fruit (90.10 mm), yield per plot (10.29 kg/plot) and yield per hectare (457.33 q) in bottle gourd. However, GA3 @ 50 mg/kg+ Boron @ 0.1% number of node per vine (24.27), number of node bearing first flower GA3 50 @ mg/kg(4.20) and NAA @ 150 mg/kg(4.20), weight of fruit NAA @ 100 mg/kg+ Boron @ 0.1% (838.0 g) , total soluble solid GA3 @ 30 mg/kg(5.04 ºBrix) and NAA @ 200 mg/kg ascorbic acid (12.90 mg/100g). The cultivar (Pusa Naveen) gives better response with the application of GA3 @ 50 mg/kg for sex expression and NAA @ 200 mg/kg+ Boron @ 0.1% for various growth and yield parameters. It can be recommended to enhance the production of bottle gourd under Garhwal valley of Uttarakhand.

Genetic Variability and Correlation Studies in Hybrids of Bottle Gourd (Lagenaria siceraria)

The present investigation was carried out with ten bottle gourd hybrid varieties including one check arranged in a randomized block design with three replications and observations were recorded on five randomly selected plants for eleven quantitative traits at Vegetable Research Farm, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P. A detailed analysis of the results on ten bottle gourd hybrid varieties revealed that from analysis of variance it is revealed that all the traits have high significant variability and thus can be used in further analysis. Shreeman 152 showed higher days to first male and first female appearance, number of branches per vine, vine length. F115 hybrid variety found the highest fruit diameter and SW906 were found high in fruit yield per and fruit yield per ha GCV were recorded higher for Fruit yield per ha, Fruit length, Vitamin C and Fruit diameter, indicating a good amount of variability present in the material under investigation. High heritability coupled with high genetic advance as percent mean is observed in Fruit yield per ha, Fruit length, Vitamin C, TSS and days to first female appearances suggesting that selection of these traits would be effective for the desired improvement in bottle gourd. A significant positive correlation was reported between fruit yield per plant with Vitamin C, TSS, fruit yield per ha. A significant negative correlation was reported between vine length and days to first harvest, days to first male and female appearances.

Relationships between Different Growth and Yield Traits in Bottle Guord [Lagenaria siceraria (mol.) Standl] with Path Coefficient Analysis over Seasons under Salt Affected Soil

This study used 23 genotypes of bottle gourd—27 F1 hybrids, 12 Parents (9 lines, and 3 testers)—during two seasons (Y1 and Y2) and pooled analyses to analyze the correlations between fruit production per plant (Kg), growth, and economic features. The observations were evaluated based on qualities that are related to growth and yield. It was discovered that the fruit yield per plant had exhibited a significant and positive phenotypic correlation with the length of the male and female flowers' pedicels, the number of primary branches per plant, the length of the vine, the number of nodes per vine, the internodal length, the duration of picking, the length of the peduncle, the length of the fruit, the average fruit's circumference, the average fruit's weight, the number of fruits per plant. The highest positive direct effect on fruit yield per plant was exerted by number of fruit per plant followed by average fruit weight at phenotypic level. Whereas, higher negative direct effects exerted by days to first fruit harvest. The soil type of experimental site was sandy loam with average fertility level and pH in the range of 7.5-8.5

Performance of Bottle Gourd (Lagenaria siceraria L.) Genotypes for Yield and Quality under Climatic Conditions of Prayagraj

An experiment on the “Performance of Bottle Gourd Genotypes i.e. (AVT-II/2019/BOGVAR-1, AVT-II/2019/BOGVAR-2, AVT-II/2019/BOGVAR-3, AVT-II/2019/BOGVA-4, AVT-II/2019/BOGVA-5, AVT-II/2019/BOGVAR-6 RITURAJ (CHECK), GREENINDIA (CHECK), NATIONAL AGRO (CHECK), SHANKAR (CHECK) for yield and quality under climatic conditions of prayagraj” was conducted during February to May, 2022, in field of Horticulture Research Farm, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj (U.P.) India. The experiment was conducted in Randomized block design, with three replications. The genotype AVT-II/2019/BOGVAR-6 recorded maximum number of female flowers (15.66), number of fruits/plant (8.88 fruits), average yield per plant (5.71 kg/plant), yield per hectare (342.44 q/ha).

Breastfeeding practices among mothers attending a tertiary care hospital in Kashmir

Background: According to world health organization (WHO), exclusive breastfeeding (EBF) for the first 6 months of life is vital for optimal growth and development of a child. During this period, other liquids or breastfeeding substitutes should not be given to infants except for medicine or oral rehydration solution. Methods: This cross-sectional study was conducted in the child health clinic of the department of community medicine, SKIMS. The study participants were mothers accompanying their infants coming for immunisation at the child health centre. A semi-structured, questionnaire was used to interview the mothers regarding breast feeding practices of their infants. The data was entered in Microsoft excel 2010 and analysed using SPSS Version 20. Results: The prevalence of EBF was 38.7%. Mixed feeding was received by 54% and 7.3% were formula-fed. Variables that were significantly associated with EBF include residence and mother’s education. Infants from rural area were more likely to be exclusively breastfed compared to those from urban area (OR=0.365; 95% CI=0.166-0.802; p=0.012). Mothers with up to 9 years of formal education were more likely to exclusively breastfed their children than those with more than 9 years of formal education (OR=0.300;95% CI=0.121-0.744, p=0.009). Conclusions: To ensure that the rate of EBF among the mothers is increased, all the stakeholders (spouse, family and government) as well as the people at work place must play their part to the best extent.

Response of Bottle Gourd (Lagenaria siceraria (Mol.) Standl.) to Integrated Nutrient Management

A field experiment was conducted during Kharif 2019 at, All India Co-ordinate Research Project, (AICRP), College of Agriculture, Odisha University of Agricultural and Technology (OUAT).The experiment consisted of twelve treatments with three replications evaluated in randomized block design. Results of field experiment revealed that the minimum days to seed germination (7.00days), maximum vine length (49.8, 187.7, 285.95 cm at 30, 60, 75 DAS, number of branches (6.18, 7.43, 8.12) at 30, 60, 75 DAS, leaf length (22.87cm), leaf width ( 21.76cm), leaf weight (48.14g), stem diameter (2.77cm), number of nodes on main vine (42.12), node bearing 1st male flower (4.16), node bearing 1st female flower (6.87), fresh wt. of fruit (1231g), dry wt. of fruit (48.75g), no. of seeds fruit-1 (448), fruit yield (28.01 q ha-1) was recorded with treatment 50%RDF+ FYM @ 7.5t ha-1+VC @ 2.5 t ha-1+ biofertilizer Consortia (T12) and minimum under control (T1).

Material compatibility of human albumin / 中国输血杂志

【Objective】 To investigate the compatibility of human albumin and its internal packaging materials of Sinopharm Lanzhou Biopharmaceutical Co., Ltd. 【Methods】 One batch of inner packaging materials (medium borosilicate glass-molded injection bottle and halogenated butyl rubber plug for injection) was extracted with 4 extraction solvents to conduct the toxicological evaluation of potential inner packaging extracts. Through the simulated acceleration test, the trend analysis of the elements in the sample and the inner surface of the glass bottle were observed, and the routine drug inspection items during the long-term stability test process were determined. 【Results】 The detection results of the leaching elements of the internal packaging materials did not exceed the limit of 50%, and the organic matter safety threshold (margin of safety, MOS) was greater than 1.0, indicating that both the leaching elements and the organic matter had no safety risk to the user under the current exposure. The results of the simulated acceleration test show that the drug will not have the risk of peeling tablets after the long-term stability condition was placed for a period of time, and the routine inspection items of the long-term stability test drugs all meet the requirements of the pharmacopoeia. 【Conclusion】 The inner packaging material has no significant impact on the quality of drugs and has good overall compatibility, making it suitable for packaging human albumin.

Preschool Children Feeding Patterns And Early Childhood Oral Health Impact In Tanah Merah, Kelantan, Malaysia

@#Introduction: Poor feeding patterns increase risk of Early Childhood Caries (ECC), which can cause negative impacts on oral health-related quality of life (OHQoL) of preschool children and parents. This study aims to assess feeding patterns of preschool children and OHQoL of the children and their parents in Tanah Merah. Methods: A cross-sectional study was conducted involving 134 preschool children aged 5-6 years old from eight private preschools, chosen by multi-staged stratified cluster sampling. Parents answered adopted self-administered questionnaire on feeding patterns and oral health-related impacts. Mann-Whitney U test and Kruskal-Wallis test were used to compare the median between groups of feeding patterns. Results: A total of 122 parents responded. Majority of the children had history of bottle-feeding (97.5%) and being given solid food during infancy (61.2%). Many children were still using bottle to drink (38.5%). Majority were still given formula milk at least three times daily (51.1%) and were feed with bottle to put to sleep (97.9%). The Child Impact Section (CIS), Family Impact Section (FIS) and Overall ECOHIS mean scores were 2.39 (SD=3.41), 0.71 (SD=1.45) and 3.10 (SD=4.41), respectively. Most common impact reported in CIS was ‘oral pain’ (26.2%) whereas in FIS, ‘parents felt guilty’ (7.4%). None of feeding patterns were found significantly different in terms of ECOHIS score median (p-value>0.05). Children OHQoL reporting by proxy should be treated as complementary. Conclusion: Many preschool children were involved in prolonged, frequent and nocturnal bottle-feeding. No significant difference in median ECOHIS scores found according to feeding patterns.

Feeding development of children with microcephaly: a descriptive study / Desenvolvimento alimentar de crianças com microcefalia: estudo descritivo

ABSTRACT Purpose: to describe feeding characteristics of children with microcephaly, encompassing the feeding route, food consistencies, and utensils used to feed them. Methods: a descriptive study approved by the Research Ethics Committee, including 34 caregivers of children diagnosed with microcephaly. They answered a structured interview on the children's feeding route, breastfeeding, utensils used to feed them, and the food consistencies, besides sociodemographic and overall development data. Descriptive statistical analyses were performed, presenting absolute and percentage frequencies and measures of central tendency and dispersion. Results: the 34 caregivers in the sample were responsible for 34 children born between 2015 and 2018, diagnosed with microcephaly, due to various causes. Of these, 33 (97.02%) were on oral feeding in their first year of life - 26 (76.44%) on breastfeeding, nine of which (26.46%) exclusively so until 6 months old. The children were served food in baby bottles, cups, spoons, and syringes. Pureed food was the consistency most accepted by the children. Conclusions: there was a low prevalence of exclusive breastfeeding until they were 6 months old, delayed progress of consistencies, use of utensils inadequate to their age, and necessary adaptations to feed children, orally.

RESUMO Objetivo: descrever características relacionadas à alimentação de crianças com microcefalia, considerando via de alimentação, consistência e utensílios utilizados. Métodos: estudo descritivo, aprovado pelo Comitê de Ética em Pesquisa. Participaram 34 cuidadores de crianças com diagnóstico de microcefalia. Foi realizada entrevista estruturada com os cuidadores quanto à via de alimentação, amamentação, utensílios para oferta e consistência do alimento, além de aspectos sociodemográficos e de desenvolvimento global. Foi realizada análise estatística descritiva, apresentada por meio de frequências absoluta e percentual, e medidas de posição e dispersão. Resultados: os 34 cuidadores que compuseram a amostra eram responsáveis por 34 crianças nascidas entre os anos de 2015 e 2018 com diagnóstico de microcefalia de causas diversas. Dessas, 33 (97,02%) alimentaram-se por via oral durante o primeiro ano de vida, sendo que 26 (76,44%) foram amamentadas, nove delas (26,46%) de forma exclusiva até o sexto mês. Para oferta do alimento foram utilizados mamadeira, copo, colher e seringa. A consistência alimentar mais aceita pelas crianças foi a pastosa. Conclusões: observa-se baixa prevalência de aleitamento materno exclusivo até o sexto mês, atraso na progressão de consistências, uso de utensílios inadequados para a idade e necessidade de adaptações para as crianças que se mantiveram em alimentação por via oral.

Association between Pacifier Use, Bottle-Feeding, and Pre-Postnatal Variables: A Cohort Study

ABSTRACT Objective: To determine the prevalence and analyze the variables associated with the use of pacifiers and/or bottles by infants up to 6 months of age. Material and Methods: Data on sociodemographic characteristics, intention to offer pacifier and bottle-feeding, pregnancy and breastfeeding (BF) variables were collected at baseline by interviews and a self-administered questionnaire among pregnant women in the 3rd trimester. After delivery, mother-baby binomials were followed by phone calls at the 1st, 3rd, and 6th months of the baby's life (n=467) to gather information on the type of delivery, baby's gender, BF in the first hour of the newborn's life, baby's weight, mother's return to work, and oral habits. Association analyses were performed using logistic regression models with a 5% significance level with the pacifier/bottle-feeding use as the outcome. Results: Most mothers (52.5%) reported their babies used bottle-feeding, 48.2% used pacifiers and 33.4% used both of them throughout the 6 months. Intention to offer pacifier and bottle-feeding was reported by 45.0% and 54.8% of the mothers at the 3rd trimester of the pregnancy, respectively. Not living in one's own residence (OR=1.53; 95%CI: 1.05-2.24) and having the prenatal intention of offering a pacifier (OR=2.50; 95%CI: 1.63-3.83) to the baby were significantly associated with pacifier use. Variables significantly associated with bottle-feeding were mother's return to work (OR=2.48; 95%CI: 1.54-3.97), baby's lower birth weight (OR=1.58; IC95%: 1.07-2.33), and prenatal intention to offer bottle-feeding (OR=2.51; 95%CI 1.56-4.04). Conclusion: About half of the babies used pacifiers or were bottle-fed, which were associated with the mother's prenatal intention to offer them to their babies and socioeconomic factors.

Prevalence of Early Childhood Caries in India: A Systematic Review and Meta-Analysis

This review was designed to assess the pooled prevalence of early childhood caries (ECC) in India. An electronic data search was done in PubMed/MEDLINE and Scopus databases in October 2020. Epidemiological surveys assessing the prevalence of ECC were included, and data on gender, geographic region, sampling strategy, feeding habits, and dmft values were extracted. The risk of bias was assessed, and a meta-analysis was performed for pooled prevalence and mean dmft values. The selection of articles, data extraction, and validity assessment were done independently by the two reviewers(ID and RV). Athird reviewer (CJ) resolved any conflict between these two reviewers. Atotal of seventy?one studies were included for quantitative analysis. The pooled prevalence of ECC estimated from 71 studies with 69,330 participants is 46.9% (confidence interval [CI] 46.5–47.2). Males had a higher prevalence of ECC (47%, CI: 46.4–47.6,40 studies, 26,840 participants) than females (43.8%, CI: 43.2–44.2,40 studies, 24,389 participants). Region?wise analysis showed the highest number of studies (44) in southern India, with a sample size of 35,988 with a pooled prevalence of 44.6% (CI: 44–45.1). The pooled mean dmft (random effect) is 2.23 (1.97–2.48), with males having a higher proportion of 2.26 compared to 2.23 in females. The pooled prevalence of ECC was 46.9%, and the pooled mean was 2.23. The results from this study state that one in every two children in India is suffering from ECC, reflecting its public health relevance.

Prevalence and Determinants of Early Childhood Caries among Children Enrolled in Anganwadi Centres in Bhilai of Central India: A Cross-Sectional Study

Introduction: Early childhood caries (ECC), which results in rapid involvement and progression of several teeth, is a growing concern in preschool children. It directly affects a child’s overall health and quality of life. Aim: To assess the prevalence and determinants of ECC among children enrolled in Anganwadi centres in Bhilai city of Chhattisgarh. Methodology: This descriptive cross?sectional study was conducted among 360 children enrolled in Anganwadi of Bhilai. An oral examination and findings were recorded in ‘dmft’ using the WHO oral health assessment form for children 2013. The mothers’ knowledge, attitude, and practice were determined by a professionally administered 10?item pre?tested short questionnaire with Cronbach’s alpha of 0.86. The data were compiled and analysed using Microsoft Excel vs. 2013 and Statistical Package of Social Science (IBM SPSS version 23; Chicago Inc., USA). Results: The prevalence of ECC was 25.83%, out of which 13.33% in boys and 12.50% in girls. Children of middle school?educated mothers have 35.48% ECC, followed by high school and primary school, that is, 24.73 and 23.66, respectively. Mothers who assisted the children in tooth brushing have less prevalence of ECC (8.15%) as compared to non?assisted (17.8%) (P = 0.044). Conclusion: Although mothers’ knowledge was appreciable regarding reasons for tooth decay, still 1/4th of the children were found to be affected by ECC. This necessitates the importance of regular/periodic screening campaigns for the children, implementing institutional preventive programmes, and referral for children to secondary/tertiary healthcare centres.

Evaluation of the effects of different 14C-urea breath test products in the diagnosis of Helicobacter pylori infection / 中华消化杂志

Objective:To evaluate the effects of the cassette 14C-urea breath test kit, scintillation sampling bottle (solid-state scintillation method) and liquid scintillation 14C-urea breath test kit in the diagnosis of Helicobacter pylori ( H. pylori) infection. Methods:From January 7 to October 28, 2020, 239 patients were enrolled who visited Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, the Second Affiliated Hospital of Zhejiang University School of Medicine and the First Affiliated Hospital of Nanchang University. All subjects first received 14C-urea breath test.Within >1 to <7 days after gas collection, mucosal tissues were taken under gastroscopy for gold standard test, including biopsy and rapid urease test (RUT). If both biopsy and RUT indicated H. pylori positive, the result of gold standard test was H. pylori positive, and if both were negative, the result of gold standard test was H. pylori negative. If the results of biopsy and RUT were inconsistent, they were not included in the subsequent analysis. Based on the results of gold standard test, the sensitivity, specificity, accuracy, positive predictive value and negative predictive value of the cassette 14C-urea breath test kit, scintillation sampling bottle, and liquid scintillation 14C-urea breath test kit in the diagnosis of H. pylori infection were analyzed. The safety of the test was evaluated by whether there were any adverse events during the test. Descriptive methods were used for statistical analysis. Results:Among the 239 subjects, 12 cases did not complete the test, 227 subjects finally completed the test. The test completion rate was 95.0% (227/239). No.008 patient was only included in the analysis of cassette 14C-urea breath test kit and scintillation sampling bottle because of lacking the result of liquid scintillation breath test. The results of gold standard test showed that among 227 patients, 87 cases were H. pylori positive, 118 cases were H. pylori negative. The results of biopsy and RUT were inconsistent in 22 cases, so they were not included in the subsequent analysis. Excluding No.008 patient, the results of gold standard test showed that 86 cases were H. pylori positive and 118 cases were negative. Based on the results of gold standard test, the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of cassette 14C-urea breath test kit, scintillation sampling bottle, and the liquid scintillation 14C-urea breath test kit in the diagnosis of H. pylori infection were 91.9%, 100.0%, 96.6%, 100.0% and 94.4%, respectively; 95.4%, 97.5%, 96.6%, 96.5% and 96.6%, respectively; and 96.5%, 99.2%, 98.0%, 98.8% and 97.5%, respectively. Only one adverse event (right upper abdominal pain after eating) occurred. Combined with the patients condition, the adverse event was determined as the onset of chronic cholecystitis and it might not be related to the test medication. Conclusions:Cassette 14C-urea breath test kit, scintillation sampling bottle, and liquid scintillation 14C-urea breath test kit have reliable performance, good safety, and high sensitivity, specificity and accuracy in the diagnosis of H. pylori infection, which are worthy of clinical application.

Violência entre parceiros íntimos, oferta de leite materno, substitutos e uso de mamadeiras no primeiro ano de vida / Intimate partner violence, breastfeeding, breastmilk substitutes and baby bottle use in the first year of life

Resumo O objetivo deste artigo é investigar a relação da violência física entre parceiros íntimos (VFPI) e a oferta de leite materno (LM), os substitutos do leite materno (SLM) e o uso de mamadeiras entre crianças entre 12 e 15 meses. Estudo transversal com mães em unidades de saúde do município do Rio de Janeiro, realizado entre junho de 2005 e dezembro de 2009. A VFPI foi identificada pela versão brasileira da Conflict Tactics Scales 1- Form R e as práticas de alimentação foram identificadas por recordatório de 24 horas. As associações foram verificadas por regressão logística mediante estimativas de razão de chances (RC) e intervalos de confiança de 95%. O leite materno foi ofertado a 58,5% das crianças e os substitutos do leite materno a 88,5%, e 70,5% das crianças usavam mamadeira. A violência física foi observada em 26,7% dos casais. Em lares onde os casais se agridem fisicamente há uma maior chance de não ofertar leite materno, maior chance de ofertar substitutos do leite materno e de usar mamadeira comparado aos lares onde não existe violência física. Os resultados chamam atenção para a necessidade de se investigar as relações intrafamiliares em casos que são identificadas práticas inadequadas de aleitamento, e de capacitar os profissionais de saúde para apoiar as famílias em situações de conflito.

Abstract This article aims to investigate the relationship between intimate partner physical violence (IPPV) and breastfeeding (BF), use of breastmilk substitutes (BMS) and bottle-feeding among children aged 12 to 15 months. This is a cross-sectional study with mothers in primary care facilities of the city of Rio de Janeiro. IPPV was identified by the Brazilian version of the Conflict Tactics Scales 1-Form R and feeding practices were identified by a 24-hour Dietary Recall. Associations were verified by logistic regression with odds ratio (OR) estimates and 95% confidence intervals. BF was offered to 58.5% of the children and BMS to 88.5%. Also, 70.5% of the children used a baby bottle. Physical violence was observed in 26.7% of couples. Households where couples physically abuse each other are more likely to not breastfeed (OR=2.14, p-value=0.030), to use breastmilk substitutes (OR=5.15, p-value=0.03) and bottle-feed (OR=2.71; p-value=0.01), when compared to households without physical violence. The results highlight the need to investigate intrafamily relationships in cases where inadequate breastfeeding practices are identified, and to enable health professionals to support families in conflict situations.

Expectation of parental control and the maintenance of bottle-feeding in childhood

Abstract Objective: To investigate association between parental locus of control (belief of individuals about what or who has control of the events of their lives) and bottle feeding habits among children from 3 to 5 years of age. Methodology: Parental locus of control validated in Brazil, and semi-structured questionnaire to obtain sociodemographic, health, and oral habit behaviors was applied to mothers of 992 preschool children. Outcome variable "use of feeding bottle" was studied according to the time of its use (≤36 months and >36 months). Simple logistic regression models were adjusted and raw odds ratios were estimated for variables of distal blocks, which contemplated parental locus of control, socioeconomic characteristics of family, and maternal habits. In the intermediate block, the variables for conditions of the child's birth and place of health care attendance during the prenatal period and early childhood were included. In the proximal block, the time of breastfeeding and pacifier use were reported. Variables were analyzed from the distal to the proximal block, and the individual analyses that presented p ≤ 0.20 remained in each model; included in the subsequent block were the variables with p ≤ 0.10, because this was a study of prevention. Results: Longer time of feeding bottle use was associated with the internal parental locus of control, mothers older than 31 years of age, white race, premature children, who used pacifiers and are treated in the private health system. Conclusions: Children who maintained the habit of feeding bottle use for a longer time were those whose mother presented an internal locus of control.

Exploration and practice of using counterpart support resources to improve scientific research level of recipient hospitals / 中华医学科研管理杂志

Objective:Exploring the " bottle neck" factors in the scientific research management of recipient hospitals, making good use of counterpart support resources to help identifying appropriate, tailored strategies of scientific research management that might improve the research capacity of recipient hospitals.Methods:Data were collected according to questionnaire survey and on-site interview, ABC classification method were used to perform statistical analysis, and " bottleneck" factors that constraint the scientific research work of the recipient hospital were summarized.Results:" Insufficient scientific research skills and lacking of talents" and " lacking of scientific research environment and recognition" are the two most prominent factors that negatively affect the scientific research capacity building of the recipient hospitals, followed closely by " the out-dated scientific research policies and lacking support from the hospital leadership" , insufficient of research platform or resources including research funding, as well as other factors. Based on such findings, this article took the First People's Hospital of Kashgar (Guangdong counterpart support) as an example, and tried to discuss some corresponding measures on how to make good use of counterpart support resources.Conclusions:The ABC classification method were used to identify the main " bottleneck" factors, and a series of effective measures that help to making good use of counterpart support resources were explored. As a result, the efficiency of the scientific research management of the recipient hospitals, which in terms of management methods, management concepts and management models, were improved.

Fatores que impedem o aleitamento materno estendido em Unidades Básicas de Saúde de Guarulhos / Factors that prevent prolonged breastfeeding in Basic Health Units in Guarulhos

A amamentação prolongada promove diversos benefícios à saúde da criança, bem como para mãe, família e sociedade. Diante disso e da escassez de estudos sobre os aspectos envolvidos na prática do aleitamento materno após o sexto de mês de vida, o presente estudo teve como objetivo conhecer os fatores que dificultam a manutenção do aleitamento materno estendido (a partir do sexto mês até dois anos ou mais). Tratou-se de um estudo transversal, realizado em quatro unidades básicas de saúde, sorteadas, localizadas no município de Guarulhos. A amostra foi composta por mães usuárias e funcionárias das unidades básicas de saúde em questão com filhos de seis a vinte e quatro meses de idade. Para a coleta de dados foram utilizados dois questionários informativos com as variáveis do estudo. As análises de regressão simples e múltipla foram usadas para investigar a associações entre as variáveis. A utilização de mamadeira e de chupeta foram fatores importantes para a interrupção do aleitamento materno estendido. No entanto, na análise ajustada, tem-se que a idade materna predispõe a interrupção da amamentação estendida. De acordo com o modelo múltiplo de regressão logística, a utilização de chupeta aumenta em quase 20 vezes a chance de não manutenção do aleitamento materno estendido e crianças que utilizam mamadeira têm esta chance aumentada em seis vezes. Conclui-se que o avançar da idade da criança, a mamadeira e a chupeta foram determinantes para a não manutenção do aleitamento materno estendido. O retorno ao trabalho e o armazenamento do leite materno foram apontados como as principais dificuldades para manutenção da amamentação.

Prolonged breastfeeding promotes several health benefits for the child, as well as for the mother, family, and society. Given this and the scarcity of studies on the aspects involved in the practice of breastfeeding after the sixth month of life, the present study aimed to discover the factors that make it difficult to maintain breastfeeding (from six monthx to two years or more). This was a cross-sectional study, carried out in four randomly selected basic health units located in the city of Guarulhos. The sample consisted of mothers and employees of the basic health units in question, with children aged between six and twenty-four months old. For data collection, two informative questionnaires with the study variables were used. Simple and multiple regression analyses were used to investigate associations between variables. The use of a bottle and a pacifier were important factors for the interruption of prolonged breastfeeding. However, in the adjusted analysis, maternal age predisposes them to interrupt of prolonged breastfeeding. According to the multiple logistic regression model, the use of a pacifier increases the chance of not maintaining prolonged breastfeeding by almost 20-fold, and children who use a bottle have this chance increased by six-fold. It is concluded that the child aging, bottle use, and pacifier use were decisive factors for not maintaining prolonged breastfeeding. Returning to work and storing breast milk were identified as the main difficulties in maintaining breastfeeding.