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Objective:To research the correlation between the cobamamide photodegradation products in the visible light area at different wavelengths and the photodegradation rate intensity. Methods:The 40-minute illumination experiment was conducted for co-bamamide reference solution respectively at the wavelength of 400 nm,450 nm,500 nm,550 nm,600 nm,650 nm and 700 nm under the illumination of 50 Lx,100 Lx,200 Lx,300 Lx,400 Lx and 500 Lx,respectively. An HPLC method was used to detect the cobam-amide content after the irradiation, the contents and proportions of hydroxocobalamin and adenosine (two photodegradation products) were calculated and the two photodegradation products were confirmed by mass spectrum. Results:The main photodegradation products of cobamamide (impurity 1,adenosine and hydroxocobalamin) had the similar photodegradation tendency. Within the scope of 50 Lx-200 Lx,the illumination intensity affected the concentration ratio of hydroxocobalamin and adenosine obviously. Within the scope of 200 Lx-500 Lx,the illumination intensity showed smaller effect. Under the different wavelengths and photodegradation rate intensity, the content of hydroxocobalamin was higher than that of adenosine under the same experimental condition.Conclusion:This paper re-searched the cobamamide photodegradation products in the optimum dark condition. The method is accurate and reliable,which can be used for the control of photodegradation related substances of cobamamide.
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Objective To observe the effect of acupuncture plus acupoint injection on the hyperhomocystinemia in the convalescent stage of cerebral infarction patients.Method A total of 120 patients in the convalescent stage of cerebral infarction with hyperhomocystinemia were randomized into group A, group B, and group C, 40 cases in each group. The three groups were intervened by conventional western medicine treatment and rehabilitation therapy, the group A was byXing Nao Kai Qiao acupuncture treatment in addition, the group B was by acupoint injection with cobamamide for injection and the group C was byXing Nao Kai Qiao acupuncture plus acupoint injection treatment. The change of the plasma homocysteine (HCY) level, the Barthel Index (BI) score and National Institutes of Health Stroke Scale (NIHSS) score were observed before and after treatment.ResultEach index(the HCY level, BI score and NIHSS score) in the three groups was significantly changed after treatment(P<0.01). Each index in the group C was significantly different from that in the group A and group B after treatment(P<0.05,P<0.01). The HCY level of the group B after the treatment was significantly different from that of the group A.Conclusion Acupuncture plus acupoint injection can significantly change the HCY level of the convalescent stage of cerebral infarction patients with hyperhomocystinemia, improve the activity of daily living and nerve function.
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Objective To improve the related substances analysis method of cobamamide.And to further research light degradation characteristics of cobamamide.Methods Determined the related substances of solid and aqueous solution of cobamamide after light degradation by high performance liquid chromatography(HPLC) conditions,which is Merck Hibar C18 column (4.6 mm ×250 mm, 5μm), 0.05 mol/L KH2PO4 solution:acetonitrile (90:10) -80% acetonitrile as mobile by gradient elution and detection wavelength 260 nm.And compared their light stability.The main three kinds of light degradation impurities were determinate from LC-MS.Results Gradient elution made the light degradation impurities separate better.The results of precision and reproducibility tests increased to RSD =0.2% (n=6) and RSD =8% (n=5) from RSD =3.8% (n =6) and RSD=38%(n=5). Cobamamide solution was very sensitive to light, the preparation should be strict dark operation.Two of the light degradation impurities were adenosine and hydroxycobalamin, with the relative response factor 2.5 and 0.7.Conclusion New method is specific, durable and reproducible, which can be used for quality control of cobamamide.
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Objective To investigate the clinical efficacy of cobamamide combined with Longxuejie in the treatment of herpes zoster in the elderly .Methods 104 elderly patients with herpes zoster were randomly divided into the treatment group and the control group .Both the two groups were given orally famciclovir 250mg,3 times a day for 14 days,intramuscularly injected gland glycosides cobalamin 500mg,1 day,14 days a course of treatment ,the treat-ment group was given Longxuejie enteric-coated tablets 4,3 times a day orally for 14 days.The blisters disappeared , pain relief,and recovery time of completely crusted ,treatment efficacy and neuralgia were compared between the two groups.Results In the treatment group,34 cases were cured,effective in 13 cases,total effective rate was 90.4%, which was significantly higher than 73.1% in the control group,the difference was statistically significant (χ2 =5.216,P<0.05).The blisters disappeared,pain relief,and recovery time of completely crusted in the treatment group were significantly shorter than those in the control group (t =3.216,5.432,4.643,3.654,all P <0.05).No postherpetic neuralgia was observed in the treatment group ,the incidence rate of postherpetic neuralgia of the control group was 13.5%,the difference was statistically significant (χ2 =4.875,P<0.05).Conclusion The Longxuejie combined with cobamamide in the treatment of herpes zoster in elderly patients has reliable effect ,fast improvement of clinical symptoms,a low incidence of postherpetic neuralgia ,it is worthy of clinical application .
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Objective To observe curative effect of sodium ozagrel combined with cobamamide and pancreatic kininogenase in patients with diabetic peripheral neuropathy (DNP). Methods Seventy-two diabetes patients with DNP in our hospital from September, 2008 to September, 2010 were randomly divided into observation group and control group. All patients were treated with cobamamide by intramuscular injection and pancreatic kininogenase taken orally. In the observation group sodium ozagrel injection were added to the treatment used in the control group. Results After one course of treatment, the total effective rate in the observation group was 91. 7% (31/36),which was better than the control group (63. 9% ,23/36) (x =7. 6, P 0. 05). After treatment, the nerve conduction velocity in the observation group significant better than those in the control group (P < 0. 05). Conclusion The treatments of sodium ozagrel combined with cobamamide and pancreatic kininogenase in diabetic peripheral neuropathy is safe and effective.