CHINA PH ARMACY Volume 33，Number 13，July 15，2022 Semimonthly Table of Contents Discussion on development difficulties and countermeasures of complex preparations in China / 中国药房

OBJECTIVE To discuss the difficulties in t he development of complex preparations and puts forward solutions ，in order to provide some reference for the development and preparation of complex preparations. METHODS Based on the development status of complex preparations ，the challenges faced by the development of complex preparations were analyzed ，and the countermeasures for the development of complex preparations were proposed. RESULTS & CONCLUSIONS The development of complex preparations required high quality of personnel ，and was limited by pharmaceutical excipients ，delivery devices and pharmaceutical equipment. The preparation process was complex ，there were many quality control parameters and patent barriers. Complex preparation development enterprises need to pay more attention to talents ，pharmaceutical excipients ，delivery devices and pharmaceutical equipment ，and clarify the impact of key technology on preparation production and quality characteristics ；in addition，they need to strengthen independent research and development ，crack the patent layout of original research enterprises and protect their own innovative achievements. Domestic complex preparation development enterprises can combine imitation and innovation to develop complex preparation products with independent brands to better meet the needs of patients.

Effects of Spirulina's Complex Preparations on Morphologies and Protein Expression of HSP70 inMyocardium,Skeletal Muscle and Liver Tissue of Mice during Prolonged Exhaustive Eexercise / 中国运动医学杂志

Objective Effect of spirulina's complex preparation(SACP) on morphologies and protein expression of HSP70 in myocardium, skeletal muscle and liver tissue were investigated in the mice during 4-week prolonged exhaustive exercise . Methods Male mice were divided into 5 groups as follow: N(control), M(exercise with moderate intensity), MS(exercise with moderate intensity plus spirulina supplementation), F(exercise with increasing intensity) and FS(exercise with increasing intensity plus spirulina supplementation). Light microscope and electron microscope were used to observe the effect of SACP on morphologies in myocardium, skeletal muscle and liver tissue in mice in group F and group FS. HSP70 protein expression was detected by immunohistochemical method. Results Under the light microscope, edema, degeneration and necroses in myocardium, skeletal muscle and liver tissues were found in the mice of group F, and fragmentation of muscle fiber, cell swelling, mitochondrial swelling and nucleoplasm solution in myocardium, skeletal muscle were found under the electron microscope in the mice of group F. The aforementioned lesions of myocardium, skeletal muscle and liver tissue in the mice of group FS were obviously alleiated. HSP70 protein expression in myocardium, skeletal muscle cells and liver cells in swimming mice was significantly higher than that in control (P0.05). Conclusions SACP had protecting effect against the microtrauma in myocardium, skeletal muscle and liver tissues caused by prolonged exhaustive exercise, and the effect was probably related to the improvement of the protein expression of HSP70 affected by SACP supplementation.