Metodología para el tratamiento de los contenidos del hockey sobre césped categoría 11-12 años

La investigación va dirigida a resolver insuficiencias en el tratamiento de los contenidos en el proceso de enseñanza-aprendizaje del hockey sobre Césped, en la categoría 11-12 años; en consecuencia, se planteó como objetivo elaborar una metodología para su proceder didáctico. De una población de 18 profesores se seleccionó, de manera intencionada, una muestra de 12; y de 168 hockeístas, a 72. Se emplearon métodos y técnicas como el análisis de documentos, la observación, la entrevista, el experimento pedagógico formativo y los talleres de opinión crítica. Se resolvió la esencia de la contradicción entre el tratamiento fragmentado de los contenidos del hockey categoría 11-12 años y la necesidad de una visión teórico y metodológica que favorezca la estructuración coherente entre el carácter desarrollador de los componentes de la preparación de los hockeístas y los del proceso de enseñanza-aprendizaje de este deporte. Se argumentaron las potencialidades instructivas y educativas de los contenidos como vía para lograr el vínculo de la unidad entre lo cognitivo y lo afectivo en la metodología que contribuya al perfeccionamiento de su estructuración, mediante procedimientos didácticos sustentados en el aprendizaje desarrollador y las particularidades del deporte. La aplicación de la metodología, desde el experimento pedagógico formativo, permitió valorar su factibilidad y funcionalidad, y se evidenció su efectividad para lograr los fines trazados.

A pesquisa tem como objetivo solucionar insuficiências no tratamento de conteúdos no processo de ensino-aprendizagem do Hóquei em Campo, na categoria de 11 a 12 anos; Consequentemente, estabeleceu-se o objetivo de desenvolver uma metodologia para seu procedimento didático. De uma população de 18 professores, foi selecionada intencionalmente uma amostra de 12; e de 168 jogadores de hóquei, para 72. Foram utilizados métodos e técnicas como análise documental, observação, entrevista, experimento pedagógico formativo e oficinas de opinião crítica. A essência da contradição entre o tratamento fragmentado dos conteúdos da categoria Hóquei 11-12 anos e a necessidade de uma visão teórico-metodológica que favoreça a estruturação coerente entre o carácter desenvolvimental das componentes da preparação dos jogadores de hóquei e da processo de ensino-aprendizagem deste esporte. O potencial instrutivo e educativo dos conteúdos foi defendido como forma de alcançar o elo de unidade entre o cognitivo e o afetivo na metodologia que contribui para a melhoria da sua estruturação, através de procedimentos didáticos apoiados na aprendizagem desenvolvimental e nas particularidades do desporto. A aplicação da metodologia, a partir da experiência pedagógica formativa, permitiu avaliar a sua viabilidade e funcionalidade, sendo evidente a sua eficácia no alcance dos objetivos estabelecidos.

The research is aimed at resolving insufficiencies in the treatment of content in the teaching-learning process of Field Hockey, in the 11-12-year-old category; consequently, the objective was set to develop a methodology for its didactic procedure. From a population of 18 teachers, a sample of 12 was intentionally selected; and from 168 hockey players, 72 were chosen. Methods and techniques such as document analysis, observation, interview, formative pedagogical experiment and critical opinion workshops were used. It was resolved the essence of the contradiction between the fragmented treatment of the contents of Hockey category 11-12 years and the need for a theoretical and methodological vision that favors coherent structuring between the developmental nature of the components of hockey players ` preparation and those of the teaching-learning process of this sport. The instructive and educational potential of the contents were argued as a way to achieve the link of unity between the cognitive and the affective in the methodology that contributes to the improvement of its structuring, through didactic procedures supported by developmental learning and the particularities of sport. The application of the methodology, from the formative pedagogical experiment, allowed its feasibility and functionality to be assessed, and its effectiveness in achieving the established goals was evident.

Phytochemical screening and evaluation of antioxidant, total phenolic and flavonoid contents in various weed plants associated with wheat crops

Abstract This study explores the antioxidant activity, phytochemical screening, total phenolic and flavonoids contents in the extracts of four locally available weeds plants namely Convolvulus arvensis, Chenopodium murale, Avena fatua and Phalaris minor with different solvents. The antioxidant activities of these extracts were determined via various in-vitro methods such as total antioxidant activity (TAA), reducing power (RP), DPPH (2,2-Diphenyl-1-Picrylhydrazyl) free radical scavenging and hydrogen peroxide scavenging assays. Phytochemical screening was performed both qualitatively as well as quantitatively. Total phenolic content (TPC) and total flavonoid content (TFC) were determined through Folin- Ciocalteu reagent and aluminium chloride methods respectively. Methanol-chloroform solvent showed the presence of a high amount of TPC in milligram of gallic acid equivalent per gram of dry weight (mg of GAE/g of DW) in the extracts of all weeds. Their descending sequence was Avena fatua (74.09) Phalaris minor (65.66) Chenopodium murale (64.04) Convolvulus arvensis (61.905), while, chloroform solvent found to be best solvent for the extraction of TFC. Methanol-chloroform solvent was also found to be best solvent for TAA (Total antioxidant activity assay) which showed values in milligram of ascorbic acid equivalent per gram of dry weight (mg of AAE /g of DW), for DPPH scavenging activity, reducing power (antioxidant activity) and hydrogen peroxide scavenging activity. Phytochemical screening indicated the presence of polyphenols, flavonoids, tannins, saponins, alkaloids and glycosides in these weeds.

Resumo Este estudo investiga a atividade antioxidante, a triagem fitoquímica, os teores de fenólicos totais e de flavonoides nos extratos de quatro plantas daninhas disponíveis localmente, quais sejam, Convolvulus arvensis, Chenopodium murale, Avena fatua e Phalaris minor com diferentes solventes. As atividades antioxidantes desses extratos foram determinadas por meio de vários métodos in vitro, tais como atividade antioxidante total (TAA), poder redutor (RP), sequestro de radicais livres DPPH (2,2-Difenil-1-Picril-hidrazil) e ensaios de sequestro de peróxido de hidrogênio. A triagem fitoquímica foi realizada tanto qualitativamente quanto quantitativamente. O teor de fenólicos totais (TPC) e o teor de flavonoides totais (TFC) foram determinados pelos métodos do reagente de Folin-Ciocalteu e do cloreto de alumínio, respectivamente. O solvente metanol-clorofórmio mostrou a presença de elevada quantidade de TPC em miligramas de ácido gálico equivalente por grama de peso seco (mg de GAE/g de DW) nos extratos de todas as plantas daninhas. Sua sequência descendente foi Avena fatua (74,09) Phalaris minor (65,66) Chenopodium murale (64,04) Convolvulus arvensis (61,905), enquanto o solvente clorofórmio foi o melhor solvente para a extração de TFC. O solvente metanol-clorofórmio também foi considerado o melhor solvente para AAT (ensaio de atividade antioxidante total), que apresentou valores em miligramas de equivalente de ácido ascórbico por grama de peso seco (mg de AAE/g de DW), para atividade sequestrante de DPPH, RP (atividade antioxidante) e atividade de sequestro de peróxido de hidrogênio. A triagem fitoquímica indicou a presença de polifenóis, flavonoides, taninos, saponinas, alcaloides e glicosídeos nessas plantas daninhas.

Inorganic contamination in roadkill birds in Northeast Brazil / Contaminação inorgânica em aves atropeladas no Nordeste do Brasil

Abstract Wildlife roadkill surveys in Brazil often focus on birds, as they are one of the main groups affected by road accidents. In addition roadkill, anthropogenic litter also contributes to a significant number of bird fatalities every year. The present study aimed to investigate the stomach contents and possible contamination by inorganic material of birds killed on a federal highway that crosses the Serra de Itabaiana National Park in the state of Sergipe, Brazil. Monitoring and data collection were carried out from January to June 2022, with a motorcycle at an average speed of 60 km/h. The collected animals were transported to the Institute of Technology and Research, located at the Tiradentes University, Sergipe, Brazil, and a plastic material was identified. The plastic material found was subjected to Fourier Transform Infrared Spectroscopy (FTIR), which showed a similar wavenumber to a polymer in the polypropylene chain, very common in plastic packaging. The presence of plastics and metals in the intestinal contents of Caracara plancus and Crotophaga ani is noteworthy, as they have a greater tendency to ingest plastics due to their generalist diet. Plastic contamination in Progne chalybea and Nyctidromus albicollis probably occurred through bioaccumulation, from the consumption of insects contaminated by microplastics.

Resumo As pesquisas sobre atropelamentos de animais silvestres no Brasil geralmente se concentram nas aves, pois elas são um dos principais grupos afetados por acidentes rodoviários. Além dos atropelamentos, o lixo antropogênico também contribui para um número significativo de mortes de aves todos os anos. O presente estudo teve como objetivo investigar o conteúdo estomacal e possível contaminação por material inorgânico de aves atropeladas em uma rodovia federal que atravessa o Parque Nacional da Serra de Itabaiana, no estado de Sergipe, Brasil. O monitoramento e a coleta de dados foram realizados no período de janeiro a junho de 2022, com uma motocicleta a uma velocidade média de 60 km/h. Os animais coletados foram transportados para o Instituto de Tecnologia e Pesquisa situado na Universidade Tiradentes, Sergipe, Brasil, onde foi identificado um material plástico. O material plástico encontrado foi submetido à espectroscopia no infravermelho com transformada de Fourier (FTIR), que mostrou número de onda semelhante a um polímero da cadeia do polipropileno, muito comum em embalagens plásticas. Chama a atenção a presença de plásticos e metais no conteúdo intestinal de Caracara plancus e Crotophaga ani, pois apresentam maior tendência à ingestão de plásticos devido à dieta generalista. A contaminação plástica em Progne chalybea e Nyctidromus albicollis provavelmente ocorreu por bioacumulação, a partir do consumo de insetos contaminados por microplásticos.

Analysis on Advantages of New Integration Processing Method in Producing Area of Polygoni Multiflori Radix Praeparata / 中国实验方剂学杂志

ObjectiveTo analyze the effects of new integration processing method in producing area and traditional method on the composition and pharmacological action of Polygoni Multiflori Radix Praeparata（PMRP）， and to illustrate the advantages of toxicity reducing and efficacy enhancing of the decoction pieces prepared by the new method. MethodFresh Polygoni Multiflori Radix（PMR） was taken from Dao-di producing area， and was processed by new integration processing method in producing area（steaming with black bean juice under pressure of 0.1 MPa and temperature at 120 ℃ for 10.5 h） and traditional method（steaming with black bean juice under water for 36 h）， respectively. Samples were collected during the processing process of the two methods， For new method， the samples were collected at 0.5， 3， 5.5， 8， 10.5 h， separately. For traditional method， the samples were collected every 4 h. High performance liquid chromatography（HPLC） was used to establish fingerprint and identify common peaks， the content of polysaccharides was determined by anthrone-sulfuric acid colorimetry at 627 nm， and the contents of anthraquinones and stilbene glycosides in different processed products were determined according to the methods under the item of determination of PMR and PMRP in the 2020 edition of Chinese Pharmacopoeia. In pharmacological experiments， 90 SD rats were randomly divided into 9 groups with 10 in each group（half of male and half of female）， including the blank group， and raw products， 24 h processed products under atmospheric pressure， 30 h processed products under atmospheric pressure， 8 h processed products under high pressure groups with low and high dosages（4.125， 16.5 g·kg-1）. Rats were given the drug by gavage for 29 d with once a day， blood was collected from the abdominal aorta after the last administration， and the serum was isolated， the body mass and liver mass of rats were weighed and the organ index was calculated. The pathological change of liver tissue was observed by hematoxylin-eosin（HE） staining， and biochemical methods were used to detect the contents of aspartate aminotransferase（AST）， alanine aminotransferase（ALT）， alkaline phosphatase（ALP）， γ-glutamyltransferase（GGT）， lactic dehydrogenase（LDH） in serum which used as liver function indicators and the levels of superoxide dismutase（SOD）， malondialdehyde（MDA）， glutathione peroxidase（GSH-Px） in brain tissues which used as oxidation indicators. ResultA total of 14 common peaks were identified in the fingerprint of PMR， PMRP prepared by new method and traditional method， and three of the peaks were designated as stilbene glycoside， emodin and emodin methyl ether， respectively. The characteristic peak areas of each processed products changed significantly from 0 min to 25 min， indicating that different processing methods had an effect on the contents of components with high polarity in PMRP， and the trend of the changes of the two methods was similar， with the higher degree of change in the new method. The determination results showed that compared with the traditional method， the content of polysaccharide（a kind of beneficial component in PMRP obtained by the new method） significantly increased， while the contents of stilbene glycoside and bound anthraquinone（liver-damaging ingredients） significantly decreased. The pharmacological results showed that compared with the blank group， AST and LDH levels of male rats in the low and high dose groups of 24 h processed products under atmospheric pressure and AST level of male rats in the low and high dose groups of 8 h processed products under high pressure were significantly reduced（P<0.05， P<0.01）， while compared with the raw product groups with the same dose， AST and LDH levels of male rats in the low dose group of 30 h processed products under atmospheric pressure were significantly reduced（P<0.05， P<0.01）， the AST levels of male rats in the low and high dose groups of 8 h processed products under high pressure were significantly decreased（P<0.01）， and there was no statistical significance in the differences of biochemical indexes of female rats in each administration group as compared with those of the blank group. ConclusionThe new integration processing method in producing area of PMRP can reach the quality of relevant regulations in 8 h. The processed products obtained by this method have more advantages than the traditional method in terms of toxicity reducing and efficacy enhancing， and energy saving to avoid the loss of ingredients， which can provide ideas for the production of high-quality decoction pieces of PMRP， and the integration processing method in producing area of other roots and rhizomes of traditional Chinese medicines.

Adaptación cultural y validación del Job Contents Questionnaire (JCQ) para trabajadores argentinos

El objetivo de este trabajo consiste en realizar la adaptación cultural y el estudio psicométrico de la versión española del Job Contents Questionnaire en trabajadores de Ciudad Autónoma de Buenos Aires y de Gran Buenos Aires. La muestra se conformó de 331 (70% es de género femenino y 30% es de género masculino, media de edad de 33,15, DS=11,84). Primero se realizó una adaptación cultural mediante la evaluación de jueces expertos y una prueba piloto. La versión adaptada se conformó de 29 ítems. Para evaluar el comportamiento de los ítems se realizó un análisis factorial exploratorio bajo la modalidad de correlaciones policóricas aplicando el método de mínimos cuadrados no ponderados (ULS) con un método de rotación Varimax. El instrumento quedó constituido por 21 ítems que se adecúan al modelo propuesto por Karasek. Los ítems que componen el factor Demandas (5), Control (6) y Apoyo Social (10). Los resultados evidencian criterios de validez aparente, de contenido y de constructo, con excelentes índices de fiabilidad (alfas ordinales mayores a .86). Además, se evidenció validez externa tomando como medida de criterio la escala de Ansiedad Estado del STAI. El instrumento presentó propiedades psicométricas satisfactorias para su aplicación en el contexto local.

This article aims to present the cultural adaptation and the psychometric study of the Spanish version of the Job Contents Questionnaire (JCQ, originally built by Karasek) for workers from the Ciudad Autónoma de Buenos Aires and Gran Buenos Aires. The sample consisted on 331 workers (70% are female and 30% are male, mean age of 33.15, SD=11,84). In the first instance, a cultural adaptation was carried out based on the evaluation of expert judges and a pilot test. The adapted version was made up of 29 items. To evaluate the behavior of the items, an exploratory factor analysis was performed under the polychoric correlation modality, applying the unweighted least squares (ULS) method with a Varimax rotation method. The instrument was made up of 21 items that fit the model proposed by Karasek. In summary, the items that make up the factor Demands (5), Control (6) and Social Support (10). The results show face, content and construct validity criteria, with excellent reliability indices (ordinal alphas greater than .86). In addition, external validity was evidenced by taking the STAI State Anxiety scale as a criterion measure. The instrument presented satisfactory psychometric properties for its application in the local context.

O objetivo deste trabalho é realizar a adaptação cultural e o estudo psicométrico da versão em espanhol do Job Contents Questionnaire em trabalhadores da Cidade Autônoma de Buenos Aires e Grande Buenos Aires. A amostra foi composta por 331 (70% do sexo feminino e 30% do sexo masculino, média de idade de 33,15, DP=11,84). Primeiramente, foi realizada uma adaptação cultural por meio da avaliação de juízes especialistas e um teste piloto. A versão adaptada foi composta por 29 itens. Para avaliar o comportamento dos itens, foi realizada uma análise fatorial exploratória sob a modalidade de correlação policórica, aplicando-se o método dos mínimos quadrados não ponderados (ULS) com um método de rotação Varimax. O instrumento foi composto por 21 itens que se enquadram no modelo proposto por Karasek. Os itens que compõem o fator Demandas (5), Controle (6) e Apoio Social (10). Os resultados mostram critérios de validade de face, conteúdo e construto, com excelentes índices de confiabilidade (alfas ordinais maiores que 0,86). Além disso, a validade externa foi evidenciada tomando-se a escala STAI State Anxiety como medida de critério. O instrumento apresentou propriedades psicométricas satisfatórias para sua aplicação no contexto local.

A way forward in pulmonary aspiration incidence reduction: ultrasound, mathematics, and worldwide data collection

Abstract Aspiration of gastric contents during induction of general anesthesia remains a significant cause of mortality and morbidity in anesthesia. Recent data show that pulmonary aspiration still accounts for many cases with implications on mortality despite technical and technological evolution. Practical, ethical, and methodological issues prevent high-quality research in the setting of aspiration and rapid sequence induction/intubation, and significant controversy is ongoing. Patients' position, drugs choice, dosing and timing, use of cricoid force, and a reliable risk assessment are widely debated with significant questions still unanswered. We focus our discussion on three approaches to promote a better understanding of rapid sequence induction/intubation and airway management decision-making. Firstly, we review how we can use qualitative and quantitative assessment of fasting status and gastric content with the point-of-care ultrasound as an integral part of preoperative evaluation and planning. Secondly, we propose using imaging-based mathematical models to study different patient positions and aspiration mechanisms, including identifying aspiration triggers. Thirdly, we promote the development of a global data collection system aiming to obtain precise epidemiological data. Therefore, we fill the gap between evidence-based medicine and experts' opinion through easily accessible and diffused computer-based databases. A better understanding of aspiration epidemiology obtained through focused global data gathering systems, the widespread use of ultrasound-based prandial status evaluation, and development of advanced mathematical models might potentially guide safer airway management decision making in the 21st century.

Hiperentorno de aprendizaje con contenidos histológicos para el desarrollo del trabajo independiente del estudiante

Introducción: la información a incluir en el hiperentorno de aprendizaje con contenidos histológicos, y la funcionalidad de este para el trabajo independiente del estudiante, deben ser concebidas integradamente, en una comunión de intereses donde el programa de la asignatura aporte el flujograma pedagógico. Objetivo: diseñar un hiperentorno de aprendizaje que contribuya al desarrollo del trabajo independiente del estudiante, con los contenidos histológicos del proceso de enseñanza-aprendizaje de la asignatura Célula, Tejidos, Sistema Tegumentario, de la carrera de Medicina, en la Universidad de Ciencias Médicas de Matanzas. Materiales y métodos: como método rector se empleó la dialéctica materialista de la filosofía marxista-leninista. Métodos teóricos: histórico-lógico, analítico-documental, inductivo-deductivo, sistémico estructural funcional, modelación. Métodos empíricos: encuesta a estudiantes, entrevista a profesores, observación a clases, selección de expertos. La población estuvo conformada por 10 profesores de la asignatura y una muestra de 290 estudiantes. El trabajo realizado abarcó dos etapas y tres fases: diagnóstico, diseño y valoración de los resultados. Resultados: con relación a requisitos de diseño, la dimensión mejor evaluada fue la usabilidad (4,98). En lo referido a habilidades cognitivas, se destaca, con buenos resultados, el indicador desarrollo de habilidades de navegación en la búsqueda de información, con un valor de 9,30. Conclusiones: el hiperentorno de aprendizaje diseñado es factible, muestra facilidad de uso y funcionalidad al dar respuesta a las necesidades de los estudiantes en la asignatura; se adapta a las posibilidades del estudiante.

Introduction: the information to be included in the learning hyper-environment with histological contents, and its functionality for the student's independent work, must be conceived in an integrative way, in communion of interests where the program of the subject contributes the pedagogical flowchart. Objective: to design a learning hyper-environment that contributes to the development of the student's independent work, with the histological contents of the teaching-learning process of the subject Cell, Tissues, Integumentary System, of the Medicine degree, at the Matanzas University of Medical Sciences of Matanzas. Materials and methods: the materialist dialectics of the Marxist-Leninist philosophy was used as the guiding method. Theoretical methods: historical-logical, analytical-documentary, inductive-deductive, systemic structural functional, modeling. Empirical methods: students survey, teachers interview, observation of classes, selection of experts. The population consisted of 10 teachers of the subject and a sample of 250 students. The work carried out covered two stages and three phases: diagnosis, design and evaluation of the results. Results: regarding design requirements, the best evaluated dimension was usability (4.98). In terms of cognitive skills, the development of navigation skills indicator in the search for information stands out with good results, and a value of 9.30. Conclusions: the designed learning hyper-environment is feasible, shows ease of use and functionality by responding to the needs of students in the subject; it adapts to the possibilities of the student.

The dynamics of genome size and GC contents evolution in genus Nicotiana / A dinâmica do tamanho do genoma e a evolução dos conteúdos de GC no gênero Nicotiana

Abstract Hybridization and Polyploidization are most common of the phenomenon observed in plants, especially in the genus Nicotiana leading to the duplication of genome. Although genomic changes associated with these events has been studied at various levels but the genome size and GC content variation is less understood because of absence of sufficient genomic data. In this study the flow cytometry technique was used to uncover the genome size and GC contents of 46 Nicotiana species and we compared the genomic changes associated with the hybridization events along evolutionary time scale. The genome size among Nicotiana species varied between 3.28 pg and 11.88 pg whereas GC contents varied between 37.22% and 51.25%. The tetraploid species in genus Nicotiana including section Polydiclae, Repandae, Nicotiana, Rustica and Sauveolentes revealed both up and downsizing in their genome sizes when compared to the sum of genomes of their ancestral species. The genome sizes of three homoploid hybrids were found near their ancestral species. Loss of large genome sequence was observed in the evolutionary more aged species (>10 Myr) as compared to the recently evolved one's (<0.2 Myr). The GC contents were found homogenous with a mean difference of 2.46% among the Nicotiana species. It is concluded that genome size change appeared in either direction whereas the GC contents were found more homogenous in genus Nicotiana.

Resumo A hibridização e a poliploidização são os fenômenos mais comuns observados em plantas, principalmente no gênero Nicotiana, levando à duplicação do genoma. Embora as mudanças genômicas associadas a esses eventos tenham sido estudadas em vários níveis, o tamanho do genoma e a variação do conteúdo de GC são menos compreendidos devido à ausência de dados genômicos suficientes. Neste estudo, a técnica de citometria de fluxo foi usada para descobrir o tamanho do genoma e o conteúdo de GC de 46 espécies de Nicotiana, e comparamos as mudanças genômicas associadas aos eventos de hibridização ao longo da escala de tempo evolutiva. O tamanho do genoma entre as espécies de Nicotiana variou entre 3,28 pg e 11,88 pg, enquanto os conteúdos de GC variaram entre 37,22% e 51,25%. As espécies tetraploides do gênero Nicotiana, incluindo as seções Polydiclae, Repandae, Nicotiana, Rustica e Sauveolentes, revelaram aumento e redução do tamanho do genoma quando comparados à soma dos genomas de suas espécies ancestrais. Os tamanhos do genoma de três híbridos homoploides foram encontrados perto de suas espécies ancestrais. A perda da grande sequência do genoma foi observada nas espécies evolutivas mais velhas (> 10 Myr) em comparação com as que evoluíram recentemente (< 0,2 Myr). Os teores de GC foram homogêneos com diferença média de 2,46% entre as espécies de Nicotiana. Conclui-se que a mudança no tamanho do genoma apareceu em ambas as direções, enquanto os conteúdos de GC foram encontrados mais homogêneos no gênero Nicotiana.

The dynamics of genome size and GC contents evolution in genus Nicotiana / A dinâmica do tamanho do genoma e a evolução dos conteúdos de GC no gênero Nicotiana

Hybridization and Polyploidization are most common of the phenomenon observed in plants, especially in the genus Nicotiana leading to the duplication of genome. Although genomic changes associated with these events has been studied at various levels but the genome size and GC content variation is less understood because of absence of sufficient genomic data. In this study the flow cytometry technique was used to uncover the genome size and GC contents of 46 Nicotiana species and we compared the genomic changes associated with the hybridization events along evolutionary time scale. The genome size among Nicotiana species varied between 3.28 pg and 11.88 pg whereas GC contents varied between 37.22% and 51.25%. The tetraploid species in genus Nicotiana including section Polydiclae, Repandae, Nicotiana, Rustica and Sauveolentes revealed both up and downsizing in their genome sizes when compared to the sum of genomes of their ancestral species. The genome sizes of three homoploid hybrids were found near their ancestral species. Loss of large genome sequence was observed in the evolutionary more aged species (>10 Myr) as compared to the recently evolved one’s (<0.2 Myr). The GC contents were found homogenous with a mean difference of 2.46% among the Nicotiana species. It is concluded that genome size change appeared in either direction whereas the GC contents were found more homogenous in genus Nicotiana.

A hibridização e a poliploidização são os fenômenos mais comuns observados em plantas, principalmente no gênero Nicotiana, levando à duplicação do genoma. Embora as mudanças genômicas associadas a esses eventos tenham sido estudadas em vários níveis, o tamanho do genoma e a variação do conteúdo de GC são menos compreendidos devido à ausência de dados genômicos suficientes. Neste estudo, a técnica de citometria de fluxo foi usada para descobrir o tamanho do genoma e o conteúdo de GC de 46 espécies de Nicotiana, e comparamos as mudanças genômicas associadas aos eventos de hibridização ao longo da escala de tempo evolutiva. O tamanho do genoma entre as espécies de Nicotiana variou entre 3,28 pg e 11,88 pg, enquanto os conteúdos de GC variaramentre 37,22% e 51,25%. As espécies tetraploides do gênero Nicotiana, incluindo as seções Polydiclae, Repandae, Nicotiana, Rustica e Sauveolentes, revelaram aumento e redução do tamanho do genoma quando comparados à soma dos genomas de suas espécies ancestrais. Os tamanhos do genoma de três híbridos homoploides foram encontrados perto de suas espécies ancestrais. A perda da grande sequência do genoma foi observada nas espécies evolutivas mais velhas (> 10 Myr) em comparação com as que evoluíram recentemente (< 0,2 Myr). Os teores de GC foram homogêneos com diferença média de 2,46% entre as espécies de Nicotiana. Conclui-se que a mudança no tamanho do genoma apareceu em ambas as direções, enquanto os conteúdos de GC foram encontrados mais homogêneos no gênero Nicotiana.

The dynamics of genome size and GC contents evolution in genus Nicotiana

Abstract Hybridization and Polyploidization are most common of the phenomenon observed in plants, especially in the genus Nicotiana leading to the duplication of genome. Although genomic changes associated with these events has been studied at various levels but the genome size and GC content variation is less understood because of absence of sufficient genomic data. In this study the flow cytometry technique was used to uncover the genome size and GC contents of 46 Nicotiana species and we compared the genomic changes associated with the hybridization events along evolutionary time scale. The genome size among Nicotiana species varied between 3.28 pg and 11.88 pg whereas GC contents varied between 37.22% and 51.25%. The tetraploid species in genus Nicotiana including section Polydiclae, Repandae, Nicotiana, Rustica and Sauveolentes revealed both up and downsizing in their genome sizes when compared to the sum of genomes of their ancestral species. The genome sizes of three homoploid hybrids were found near their ancestral species. Loss of large genome sequence was observed in the evolutionary more aged species (>10 Myr) as compared to the recently evolved ones ( 0.2 Myr). The GC contents were found homogenous with a mean difference of 2.46% among the Nicotiana species. It is concluded that genome size change appeared in either direction whereas the GC contents were found more homogenous in genus Nicotiana.

Resumo A hibridização e a poliploidização são os fenômenos mais comuns observados em plantas, principalmente no gênero Nicotiana, levando à duplicação do genoma. Embora as mudanças genômicas associadas a esses eventos tenham sido estudadas em vários níveis, o tamanho do genoma e a variação do conteúdo de GC são menos compreendidos devido à ausência de dados genômicos suficientes. Neste estudo, a técnica de citometria de fluxo foi usada para descobrir o tamanho do genoma e o conteúdo de GC de 46 espécies de Nicotiana, e comparamos as mudanças genômicas associadas aos eventos de hibridização ao longo da escala de tempo evolutiva. O tamanho do genoma entre as espécies de Nicotiana variou entre 3,28 pg e 11,88 pg, enquanto os conteúdos de GC variaram entre 37,22% e 51,25%. As espécies tetraploides do gênero Nicotiana, incluindo as seções Polydiclae, Repandae, Nicotiana, Rustica e Sauveolentes, revelaram aumento e redução do tamanho do genoma quando comparados à soma dos genomas de suas espécies ancestrais. Os tamanhos do genoma de três híbridos homoploides foram encontrados perto de suas espécies ancestrais. A perda da grande sequência do genoma foi observada nas espécies evolutivas mais velhas (> 10 Myr) em comparação com as que evoluíram recentemente ( 0,2 Myr). Os teores de GC foram homogêneos com diferença média de 2,46% entre as espécies de Nicotiana. Conclui-se que a mudança no tamanho do genoma apareceu em ambas as direções, enquanto os conteúdos de GC foram encontrados mais homogêneos no gênero Nicotiana.

Species differences of Baishouwu based on characteristic chromatogram and content determination of acetophenones / 药学实践杂志

Objectives To provide scientific basis for the quality control of different species of Baishouwu by establishing the HPLC fingerprint of domestic of Baishouwu and determining the main active components of acetophenones. Methods HPLC-DAD method was used to determine the HPLC fingerprints of domestic of Baishouwu. Then, the content of 4 kinds of acetophenones in Baishouwu was determined. The column was Diamonsil C18（250mm×4.6mm, 5μm）with the mobile phase of methanol and 0.1% phosphoric acid at a flow rate of 1.0 ml/min. The detection wavelength of p-Hydroxyl acetophenone, baishouwu benzophenone, 2',4'-Dihydroxy acetophenone was set at 260 nm and 2',5'-Dihydroxy acetophenone at 280 nm respectively. Results The similarity and cluster analysis in HPLC fingerprint showed that the constituents were significantly different among C. bungei, C. auriculatum and C. wilfordii. The content of total acetophenones in C. wilfordii was significantly higher than that in other localities of C. auriculatum and C. bungei. Conclusions Acetophenone could be used as the evaluation index to evaluate the quality of Baishouwu in different origins. The content of total acetophenone in C. wilfordii is the highest, which could be used as the best quality resource of Baishouwu.

Identifying the pharmaceutical formulation of gynecological khii bureldsen disease in the Mongolian traditional medicine from previous reports / Монголын эм зүй, эм судлал

Introduction@#A gynecoloic qisu syndrome lasts for long time and shows signs of chronic khii bureldsen disease and causes a gynecological khii bureldsen disease at whole-body level. The disease often occurs in hospitals, and it is one of the main gynecological diseases in Mongolian traditional medicinal hospitals³. The problem of diagnosing and treating the disease with traditional medicine is the reason for the research. @*Methods@#The documentary research method was used, and the main research materials were in Mongo- lian scripts of Ikh Yuteg Yondongombo, “Four Medical Tantras”², Luvsanchoympil “Jeduininkhor” ⁴, Disren Sanjaajamtsin “Khukh Binderiya” ⁵, “Pharmaceutical Formulation of Mongolian Medicine” ¹³ As auxiliary materials, Mongolian scripts of the Mongolian editions of Jigmeddanzinjamts “Tun- gagajad” ³, Yondongjamts “Treatment Booklet of Tibetan Medicine” ⁶, Jambalchoynkhor “Four Keys of the Sea of Charms” ⁷, Jigmeddanzanjamts “Courageous Treatment Concepts” ¹⁰, and Ishbaljir “Four Springs” ¹¹ were used.</br> At the meeting of the academic council of the Inner Mongolian National University, permission was obtained to conduct the research. Furthermore, since this research is documentary research, we have taken care not to violate the copyright by mentioning the name of the author of the book or work every time it is quoted.@*Conclusion@#Due to the improper food and drinks that increase khii /rlung/, gynecological khii bureldsen disease formation is caused by the longtime of untreated or incorrect treatment, and the disease develops slowly and chronically. As causes, conditions, pathophysiology, and differentiation between the causes of gynecological khii bureldsen diseases are similar in meaning, it shows that the ancient reports and works are related to each other.</br> In gynecological khii bureldsen disease, the selection of medicine with warm characteristics to suppress the rlung is based on the yin yang theory of Eastern philosophy which is the main method to suppress the cool characteristics of rlung.

Effectiveness of a Decision Support Training Program for Health Care Professionals

Purpose: In today’s medical field, it is an essential quality competency for staff to not only recognize the importance of patient decision-making and the skills to support it, but also implement it. This study aimed to establish a training program on decision support for healthcare professionals and examine its effectiveness. Method: We conducted the training at a medical institution and conducted a questionnaire survey at two points before and after the training. A total of 88 nurses and doctors participated in the survey. Result: We developed a two-hour training on the knowledge and skills needed for decision support. Questionnaire results showed improvements in literacy and efficacy before and after the training. Discussion: It was confirmed that the training led to an in-depth understanding of the participants’ decision support, and increased the sense of efficacy in their daily work, particularly through responding to patients according to patients’ cognitive and physical assessments, and in actively supporting those who have difficulty in making decisions. There were references to the significance of re-learning and the possibility of applying the training to difficult situations in participants comments. In the future, it is necessary to study decision support with reference toco-operation in the medical field where collaboration among multiple professions is indispensable.

Hot water washing processing technology of Euodiae Fructus based on change laws of active components and tastes / 中国中药杂志

In order to establish the standardized processing technology of the hot water washing of Euodiae Fructus, this study, based on the traditional processing method of hot water washing of Euodiae Fructus recorded in ancient works and modern processing specifications of traditional Chinese medicine decoction pieces, took the yield of decoction pieces and the content of main components as the indicators and optimized the processing conditions by orthogonal test based on the results of single factor investigation. At the same time, electronic tongue technology was used to analyze the change law of the taste index of Euodiae Fructus during the hot water washing. The results of the single factor investigation showed that the content of the main components in Euodiae Fructus showed some regular changes during the processing. Specifically, the content of chlorogenic acid, hyperin, isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-galactoside, and dehydroevodiamine decreased significantly, with average decreases of-23.75%,-27.80%,-14.04%,-14.03%, and-13.11%, respectively. The content of limonin increased significantly with an average increase of 19.83%. The content of evodiamine, rutaecarpine, evocarpine, and dihydroevocarpine showed fluctuating changes and generally increased, with average variation amplitudes of 0.54%,-3.78%, 2.69%, and 5.13%, respectively. The orthogonal test results showed that the optimum processing parameters for the hot water washing of Euodiae Fructus were as follows: washing time of 2 min, the solid-to-liquid ratio of 1∶10 g·mL~(-1), washing temperature of 80 ℃, washing once, and drying at 50 ℃. After the hot water washing processing, the average yield of Euodiae Fructus pieces was 94.80%. The content of limonin, evodiamine, and rutaecarpine was higher than those of raw pro-ducts, and the average transfer rates were 102.56%, 103.15%, and 105.16%, respectively. The content of dehydroevodiamine was lower than that of the raw products, and the average transfer rate was 83.04%. The results of taste analysis showed that the hot water washing could significantly reduce the salty, astringent, and bitter tastes of Euodiae Fructus. This study revealed the influence of the hot water washing on the content of main components and taste of Euodiae Fructus, and the processing technology of the hot water was-hing of Euodiae Fructus established in this study was stable, feasible, and suitable for industrial production, which laid a foundation for clarifying its processing principle and improving the quality standard and clinical application value of decoction pieces.

Systematic construction of undergraduate curriculum for rehabilitation based on WHO rehabilitation competency framework / 中国康复理论与实践

ObjectiveTo systematically develop the courses of rehabilitation based on World Health Organization rehabilitation competency framework (RCF). MethodsThe theories and methods of RCF were used to analyze competency characteristics of rehabilitation workers and educational objectives to construct the curriculum contents in rehabilitation. ResultsThe competency of the rehabilitation workers included practice, professionalism, learning and development, management and leadership, research, values and beliefs in the specific rehabilitation situations. The objectives of rehabilitation medicine education needed to correspond to the competencies of the rehabilitation workers and set up knowledge units that could develop competency in terms of behaviour, activity tasks and proficiency. The curriculum systems of rehabilitation with RCF included the fields of rehabilitation physiotherapy, rehabilitation occupational therapy, hearing and speech rehabilitation, and prosthetic and orthopaedic engineering, etc. ConclusionThe teaching objectives corresponding to the competency characteristics of rehabilitation workers need to be translated into competency-based education. The curriculum contents in rehabilitation needs to be systematically constructed based on RCF from the areas of rehabilitation physiotherapy, rehabilitation occupational therapy, hearing and speech rehabilitation, and prosthetic and orthopaedic engineering.

Preoperative fasting for the infusion of "yerba mate": a randomized clinical trial with ultrasound evaluation of gastric contents

Abstract Background The traditional infusion of "yerba mate" is widely consumed in South America and exported to countries around the world. Although generally considered a "clear fluid", there is no data to date on the gastric emptying time of yerba mate and safe preoperative fasting intervals. The objective of this study was to evaluate the gastric emptying time of a standardized infusion of yerba mate using bedside ultrasound and compare it with the time confirm of hot and cold tea. Methods This was a prospective, randomized crossover experimental study. Thirty healthy volunteers were evaluated after 8 hours of fasting for both fluids and solids. Gastric antral area and gastric volume were evaluated at baseline and every 20 minutes after drinking 300 mL of randomly assigned infusion of "yerba mate", hot tea, or cold tea. Results The mean gastric emptying time was: 69.7 ± 22.1 min, 63.1 ± 14.5 min, and 64.3 ± 23.5 min for the mate, hot tea, and cold tea respectively. No significant differences were found in emptying time among the infusion groups (p-value = 0.043). When same time measures were compared, the only significant difference detected was between hot teas and mate infusion at 20 minutes (p-value = 0.012) Conclusion Yerba mate infusion has a similar gastric emptying time to that of tea. All subject's gastric volume returned to baseline values by 100 minutes. It is reasonable to recommend a similar fasting period of 2 hours for mate infusion prior to elective surgery.

Diagnóstico del proceso de formación técnica en la lucha olímpica / Diagnóstico do processo de treinamento técnico na luta livre olímpica / Diagnosis of the technical training process in Olympic wrestling

RESUMEN Introducción: Los organismos internacionales y nacionales exigen un proceso de formación que facilite el acceso a los conocimientos técnicos, desde los métodos modernos de enseñanza, que sea inclusivo, equitativo y de calidad. Objetivo: En atención a ello, la investigación se orientó a diagnosticar el estado actual del proceso de formación técnica de las luchas olímpicas. Materiales y métodos: Con este propósito, se realizó un estudio longitudinal desde el año 2012 hasta 2021, seleccionándose como muestra a 18 directivos, 59 luchadores y 24 entrenadores. Se utilizaron dos teléfonos móviles para grabar y obtener las imágenes de los combates, desde donde se realizaron las observaciones de la técnica. Los métodos utilizados fueron el dialéctico-materialista, así como los de orden teórico y empíricos, se destacan la revisión de documentos, la entrevista, la encuesta y la observación, mientras como herramienta de constatación la triangulación metodológica, fundamental en el proceso de descripción del objeto de estudio. Resultados: Los procedimientos aplicados determinaron la existencia de dificultades en la dirección pedagógico-deportiva, el sistema de contenidos científico-técnicos y el análisis de factores claves de la técnica, en especial, con manejo del modelo acción (librería de video), el video-análisis (parámetros de ejecución), el factor común (selección de casos exitosos y no exitosos) y los documentos de trabajo (fichas técnicas y listado cotejo). Conclusiones: Finalmente, se declaran las pautas de un modelo didáctico para la formación de la técnica, con base en una elaboración sistémica de los contenidos científico técnicos y el análisis de factores claves, considerando las tecnologías de la información y las comunicaciones (Tic).

SÍNTESE Introdução: Organismos internacionais e nacionais exigem um processo de treinamento que facilite o acesso ao conhecimento técnico, a partir de métodos modernos de ensino, que seja inclusivo, equitativo e de qualidade. Objetivo: Em vista disto, a pesquisa teve como objetivo diagnosticar o estado atual do processo de treinamento técnico na luta livre olímpica. Materiais e métodos: Para este fim, foi realizado um estudo longitudinal de 2012 a 2021, selecionando 18 gerentes, 59 lutadores e 24 treinadores como amostra. Dois telefones celulares foram usados para gravar e obter as imagens dos surtos, de onde foram feitas as observações da técnica. Os métodos utilizados foram dialético-materialista, bem como métodos teóricos e empíricos, incluindo revisão de documentos, entrevista, levantamento e observação, enquanto que a triangulação metodológica foi utilizada como ferramenta de verificação, fundamental no processo de descrição do objeto de estudo. Resultados: Os procedimentos aplicados determinaram a existência de dificuldades na direção pedagógico-desportiva, o sistema de conteúdo técnico-científico e a análise de fatores-chave da técnica, em particular, com o manuseio do modelo de ação (videoteca), a vídeo-análise (parâmetros de execução), o fator comum (seleção de casos de sucesso e insucesso) e os documentos de trabalho (fichas técnicas e lista de verificação). Conclusões: Finalmente, são apresentadas as diretrizes de um modelo didático de treinamento técnico, baseado em uma elaboração sistêmica dos conteúdos técnico-científicos e na análise de fatores-chave, considerando as tecnologias de informação e comunicação (TIC).

ABSTRACT Introduction: International and national organizations demand a training process that facilitates access to technical knowledge, from modern teaching methods, that is inclusive, equitable and of quality. Objective: In response to this, the research was aimed at diagnosing the current state of the technical training process of Olympic wrestling. Materials and methods: For this purpose, a longitudinal study was carried out from 2012 to 2021, selecting 18 managers, 59 wrestlers and 24 coaches as a sample. Two mobile phones were used to record and obtain the images of the combats, from where the observations of the technique were made. The methods used were the dialectical-materialist, as well as those of a theoretical and empirical nature, highlighting the review of documents, the interview, the survey and the observation, while as a verification tool the methodological triangulation, fundamental in the process of describing the object of study. Results: The applied procedures determined the existence of difficulties in the pedagogical-sports direction, the scientific-technical content system and the analysis of key factors of the technique, especially, with handling of the action model (video library), the video -analysis (execution parameters), the common factor (selection of successful and unsuccessful cases) and working documents (technical sheets and comparison list). Conclusions: Finally, the guidelines of a didactic model for the trainig of the technique are declared, based on a systemic elaboration of the scientific-technical contents and the analysis of key factors, considering the information and communication technologies (ICT).

Change in leaf anatomy, physiology, and essential oil of Varronia curassavicaJacq. accessions under two light conditions / Cambio en la anatomía de la hoja, fisiología y aceite esencial de accesiones de Varronia curassavica Jacq. bajo dos condiciones de luz

The aim of this study was to evaluate the effect of light on anatomy, physiology and essential oil content and composition of Varronia curassavica. They were analysed two light conditions (full sunlight and protected environment with 50% shade screen) and five accessions (VCUR-101, VCUR-102, VCUR-201, VCUR-302, VCUR-802). V. curassavica cultivated in full sun presented a greater development of the leaf blade and palisade parenchyma thickness for all accessions. Chlorophyll levels did not differ according to the two light environments. The leaf area was larger in the protected environment. The essential oil yield of the accessions ranged from 0.26 to 0.87 mL/plant in full sun and from 0.34 to 0.53 mL/plant in the protected environment. The composition of the essential oil was influenced by the light and the accession. All accessions presented (E)-caryophyllene and α-humulene. The influence of light on the evaluated variables is genotype dependent.

El objetivo de este estudio fue evaluar el efecto de la luz sobre la anatomía, fisiología, el contenido y composición de aceites esenciales de Varronia curassavica. Se analizaron dos condiciones de luz (pleno sol y ambiente protegido con 50% de pantalla de sombra) y cinco accesiones (VCUR-101, VCUR-102, VCUR-201, VCUR-302, VCUR-802). V. curassavica cultivada a pleno sol presentó mayor desarrollo del limbo foliar y espesor del parénquima en empalizada para todas las accesiones. Los niveles de clorofila no difirieron según los dos entornos de luz. El área foliar fue mayor en el ambiente protegido. El rendimiento de aceite esencial de las accesiones varió de 0,26 a 0,87 mL/planta a pleno sol y de 0,34 a0,53 mL/planta en el ambiente protegido. La composición del aceite esencial fue influenciada por la luz y la accesión. Todas las accesiones presentaron (E)-cariofileno y α-humuleno. La influencia de la luz sobre las variables evaluadas depende del genotipo.

Variation in phenolic, antioxidant and vitamin amounts among some medicinal plants and investigation by PCA analysis: Lamiaceae family / Variación en las cantidades de fenoles, antioxidantes y vitaminas entre algunas plantas medicinales e investigación poranálisis PCA: familia Lamiaceae

Aromatic and medicinal plants are of great importance to determine the contents of the active compounds of plant origin and to evaluate them depending on variety and climate factors in order to determine the phenolic, antioxidant enzyme activity, vitamin contents in species belonging to the Lamiaceae family. Examination of the characteristics of different species, the highest peroxidase (POD) enzyme activity, ascorbate peroxidase (AxPOD), total antioxidant (TA), malondialdehyte (MDA), caffeic acids (CA), vitamin C contents,and chloric acid (ChA) were obtained in the M. longifoliaspecies. The highest vitamin E and catalase (CAT) were determined in the S. hortensisspecies but the highest total phenolic (TP), superoxide dismutase (SOD) enzyme, hydrogen peroxide (H2O2) and chlorogenic acid (ChgA) were determined in the S. spicigeraspecies. As a result of PCA analysis, it can be said that Mentha longifolia(L.) Hudson and Satureja spicigeraspecies have significant value in terms of biochemical and phenolic content.

Las plantas aromáticas y medicinales son de gran importancia para determinar el contenido de los compuestos activos de origen vegetal y evaluarlos en función de la variedad y factores climáticos con el fin de determinar la actividad enzimática fenólica, antioxidante, contenido vitamínico en especies pertenecientes a la familia Lamiaceae. El examen de las características de diferentes especies, la mayor actividad enzimática de peroxidasa (POD), ascorbato peroxidasa (AxPOD), antioxidante total (TA), malondialdehído (MDA), ácidos cafeicos (CA), contenido de vitamina C y ácido clorhídrico (ChA) se obtuvieron en la especie M. longifolia. La mayor cantidad de vitamina E y catalasa (CAT) se determinó en la especie S. hortensis, pero la mayor cantidad total de enzima fenólica (TP), superóxido dismutasa (SOD), peróxido de hidrógeno (H2O2) y ácido clorogénico (ChgA) se determinó en la especie S. spicigera. Como resultado del análisis de PCA, se puede decir que las especies Mentha longifolia(L.) Hudson y Satureja spicigeratienen un valor significativo en términos de contenido bioquímico y fenólico.

Effects of Age and Genotype on Vitamin C (Ascorbic acid) and Fiber Contents in Brassica napus

Rape (Brassica napus) is one of the major vegetables grown in Zambia and is an important source of nutrients in human diet. Vitamin C is an essential antioxidant molecule while fiber on the other hand plays a key role in mitigating constipation. However, the effects of rape genotype and the vegetative maturity (age) on nutrient content on vitamin C and fiber is not clearly understood. The objectives of the study were therefore, to; i) to evaluate the effects of age on vitamin C and fiber nutrient content in rape leaves and ii) evaluate the genotypic effect of rape leaves on vitamin C and fiber contents. The experiment was conducted Munachoonga farm, in Monze district, Zambia (16 o 16’ 45’’ S; 27o 28’25’’ E) during the 2020/ 2021 cropping season. The experiment was laid as a split plot design with three replications. Soil fertilizer amendments were the main plot while variety (genotype) as a subplot. Data was collected at 6 and 10 weeks old after transplanting. However, for each treatment, a representative sample was collected by thoroughly mixing all three associated reps and obtaining a representative leaf sample giving 18 experimental units. The dried samples were taken to the University of Zambia Lab for analysis. The results showed that the mean fiber content was higher at week 10 than week 6 (P< 0.001), while vitamin C was higher at week 6 than week 10 (P= 0.05). Implying that younger leaves are likely to supply a higher content of vitamin C than older leaves. The lesser content of fiber at younger stage of rape explains why most consumers found rape more palatable at a younger stage. It was also revealed that vitamin C and fiber nutrient contents did not depend on the type of genotype. Furthermore, a study to evaluate the precise effects of fertilizer amendment on vitamin C and fiber contents should be undertaken.