HDCGUnet:a neural network for image segmentation of calcium imaging / 军事医学

Objective To build a neural network based on the Unet infrastructure for recognition and segmentation of two-dimensional calcium imaging fluorescence images.Methods The in vivo miniaturized two-photon microscope(mTPM)was used for brain calcium imaging in freely moving mice.The imaging data was motion corrected using the NoRMCorre algorithm and processed using ImageJ software to obtain the original images after correction,and the labels were produced using the Labelme software.The neural network HDCGUnet was built using the original images and labels for training,and optimized to improve the model structure according to the training effect.Finally,the evaluation indexes were selected and compared with those of other models to verify the utility of this model.Results The HDCGUnet model,which was collected and made on our own,performed best in the two-photon calcium imaging dataset compared to other models,and performed well on the BBBC dataset either.Conclusion The HDCGUnet model provides a novel alternative for the recognition and segmentation of two-photon calcium imaging images.

A case of mitochondrial and peroxisome fission deficiency-related encephalopathy caused by DNM1L gene mutation / 中华神经科杂志

Mitochondrial and peroxisome fission deficiency-related encephalopathy caused by DNM1L gene mutation is a rare and fatal epileptic encephalopathy, with clinical phenotype and genetic heterogeneity. The acute stage is drug-resistant epilepsy with poor prognosis and serious neurological sequelae. A case of genetically confirmed encephalopathy related to mitochondrial and peroxisome fission defects is reported, the clinical data, treatment process are summarized, and the previous literature is reviewed to improve the understanding of the rare disease.

A Comprehensive Review on Role of Bio- Regulators in the Growth and Development of Fruit and Vegetable Crops

This comprehensive review focuses on the role of bio-regulators in the growth and development of fruit and vegetable crops. Bio-regulators, also known as plant growth regulators or plant hormones, play a crucial role in regulating various physiological processes in plants, including cell division, elongation, differentiation, and fruit ripening. The review explores the major types of bio-regulators commonly used in fruit and vegetable cultivation, including auxins, gibberellins, cytokinins, abscisic acid, and ethylene. It examines their functions and effects on plant growth, flowering, fruit set, fruit development, and post-harvest characteristics. The review also discusses the widespread application of bio-regulators in horticultural practices, including seed treatment, foliar application, and post-harvest treatments. It highlights the potential benefits of using bio-regulators, such as improving crop yield, enhancing fruit quality traits, delaying senescence, and reducing post-harvest losses. Moreover, the review delves into the mechanisms of action of bio-regulators at the molecular, physiological, and biochemical levels. It investigates their interactions with various plant growth processes, signaling pathways, and gene expression patterns. Furthermore, the review addresses the challenges and limitations associated with the use of bio-regulators in fruit and vegetable production, such as dosage optimization, application timing, and potential negative impacts on the environment.

Phytodiversity of Herbaceous Flora in the Grasslands of Jannaram Division, Kawal Tiger Reserve, Telangana, India

Aims: To study the diversity and composition of herbaceous species in the grasslands of Jannaram division, Kawal Tiger Reserve, Telangana, India.Place and Duration of Study: Grasslands of Jannaram division, Kawal Tiger Reserve, Telangana, July 2022 to August 2023.Methodology: The study was carried out by laying out 223 sample plots in the Jannaram division by dividing Jannaram division into 3 ranges. The plot size was 1 x 1 m. Varied diversity indices such as Simpson, Shannon–Weiner, species evenness, and IVI were calculated according to standard formulae. Vegetation analysis and IVI value of each species were calculated and analysed.Results: A total of 65 species, comprising 53 genera and 21 families, were reported. The dominant family in the study area was Poaceae, followed by Fabaceae, Malvaceae, Acanthaceae, Asteraceae and Euphorbiaceae. The Shannon–Weiner index was 3.46 and the Simpson index was 0.05, indicating high diversity with less dominance of herb layer in the study area. Top five species holding highest IVI value are Iseilema laxum (29.94), Cynodon barberi (12.19), Eragrostis amabilis (12.15), Dichanthium annulatum (11.20), and Apluda mutica (10.21).Conclusions: This research produced fundamental information regarding the types of herbaceous plants and grasses present at the Jannaram, Kawal Tiger Reserve. This would serve as a guide to the systematists, ethnobotanists, amongst several others researchers who are interested in identifying.

Trabajo reproductivo en Cuba, una mirada desde la Encuesta Nacional de Salud / Reproductive Work in Cuba, a View from the National Health Survey

Introducción: La división sexual del trabajo diferencia el remunerado del no remunerado. Este último ha sido ejercido más frecuentemente por las mujeres, en detrimento de sus oportunidades y su salud. Objetivo: Identificar las características del trabajo no remunerado y el apoyo social, según variables sociodemográficas en la población cubana. Métodos: Se realizó un estudio descriptivo transversal en el que participaron 9 321 061 cubanos de 15 años y más; 7026 hombres y 7313 mujeres, como parte de la Encuesta Nacional de Salud 2018-2020. Se aplicó un cuestionario estructurado en los hogares para la exploración de las variables relacionadas con la responsabilidad en la realización de las tareas de hogar, cuidados de hijos menores, personas mayores, discapacitadas y apoyo social, sexo, grupos de edad y zona de residencia. La información se resumió en porcentajes y sus IC 95 por ciento. Resultados: Hubo predominio femenino en la realización de las tareas del hogar y del cuidado de niños, discapacitados y personas mayores, sin diferencias por zonas de residencia. El apoyo social recibido fue similar para hombres y mujeres. A medida que aumentó la edad disminuyó la posibilidad de recibir amor y afecto y de contar con personas para intercambiar. Conclusiones: Prevaleció una distribución inequitativa del trabajo doméstico, del cuidado de los miembros de la familia y el apoyo social percibido por la población a lo largo del curso de la vida, con peores resultados a medida que aumentó la edad, lo que reclama la implementación de estrategias más efectivas con perspectiva de género(AU)

Introduction: The gender division of labor differentiates it between paid and unpaid. The latter has been exercised more frequently by women, to the detriment of their opportunities and health. Objective: To identify the characteristics of unpaid work and social support according to sociodemographic variables in the Cuban population. Methods: A cross-sectional descriptive study was conducted with the participation of 9,321,061 Cubans aged 15 years and older; 7026 men and 7313 women, as part of the National Health Survey 2018-2020. A structured questionnaire was applied in the homes to explore the variables related to responsibility in the performance of household chores, care of minor children, the elderly, the disabled, and social support, sex, age groups and area of residence. The information was summarized in percentages and their 95percent CIs. Results: Women predominated in the performance of household chores and care for children, the disabled and the elderly, with no differences by area of residence. The social support received was similar for men and women. As age increased, so did the possibility of receiving love and affection and of having people to exchange. Conclusions: An unequal distribution of domestic work, care of family members and social support perceived by the population throughout the life course prevailed, with worse results as age increased, which calls for the implementation of more effective strategies with a gender perspective(AU)

A study of high division of sciatic nerve in human cadavers and its clinical significance

Background: Sciatic nerve is a mixed nerve which provide sensory and motor supply for skin and muscles of the lower limb by tibial and common peroneal nerve. Anatomical variations of sciatic nerve at high division have been reported by various authors. The path of the sciatic nerve is important while administration of intramuscular injection to prevent nerve injury and nerve blockage failure during anesthesia. This knowledge of high division helps in different surgical approach for sciatic nerve injury or hip dislocation. Aims and Objectives: The aim of the study was to describe incidences of high division variation of Sciatic nerve and different types in cadavers during routine dissection schedule. Materials and Methods: The study was conducted during routine dissection schedule in anatomy department for first MBBS students to observe sciatic nerve course in 30 gluteal regions from 15 adult cadavers fixed by formalin. Location of sciatic nerve in relation to piriformis muscle and its division whether in single nerve sheath or separate sheath and types was recorded. Results: In 12 cadavers (80%), sciatic nerve course found normal which leaves pelvis at inferior border of piriformis muscle and bifurcate in terminal branches tibial nerve and common peroneal nerve as it approaches at the apex of popliteal fossa. In 3 cadavers (20%), two male and one female, we found high division of sciatic nerve where terminal branches, tibial nerve, and common peroneal nerve leave the pelvis below piriformis separately in different sheaths. Conclusion: Knowledge of variations-related high division of sciatic nerve would help surgeons during different interventions related to sciatic nerve and for preventing further complications.

Maternal liver damage induced by cadmium exposure in pregnant mice through hypoxia inducible factor-1α-mediated upregulation in DRP1 / 环境与职业医学

Background Mitochondrial dynamin-related protein 1 (DRP1) regulates mitochondrial division and plays an important role in maintaining hepatocyte function. However, the role of DRP1 in cadmium exposure-induced maternal liver damage in pregnant mice remains unclear. Objective To investigate the role and mechanism of DRP1 in maternal liver damage induced by cadmium exposure during pregnancy. Methods This study consisted of animal experiments and cell experiments. (1) Animal experiments. Mice at 14 days of gestation were randomly divided into three groups: a control group, a low-dose cadmium group (LCd group: 2.5 mg·kg−1), and a high-dose cadmium group (HCd group: 5 mg·kg−1). The pregnant mice were intraperitoneally injected with cadmium chloride (CdCl2) for 6 and 24 h in the next morning. The weights of pregnant mice, uterus, maternal liver, and fetal mice were recorded after sacrifice. Serum and liver of pregnant mice were collected, the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were detected, and liver tissues were stained with HE to observe changes in liver function and liver tissue structure. The expressions of oxidative phosphorylation-related proteins, hypoxia inducible factor-1α (HIF-1α) and DRP1 proteins in liver of pregnant mice were detected by Western blotting. (2) Cell experiments. AML12 cells were treated with CdCl2 (10 μmol·L−1) for 0, 2, 6, 12, and 24 h. The expressions of oxidative phosphorylation-related proteins, DRP1, and hypoxia inducible factor-1α (HIF-1α) proteins were detected. AML12 cells were pretreated with DRP1 inhibitor Mdivi-1 for 1 h and then CdCl2 (10 μmol·L−1) for 12 h to detect the expression of oxidative phosphorylation-related proteins and DRP1 protein. AML12 cells were treated with Hif-1α siRNA for 48 h and CdCl2 (10 μmol·L−1) for 6 h to detect the expression of HIF-1α and DRP1 proteins. Results The results of animal experiments showed that cadmium exposure in pregnant mice had no effects on maternal liver weight and liver coefficient. However, the histomorphological changes and necrosis in hepatocytes were observed. Compared with the control group, the serum ALT and AST levels of pregnant mice in the LCd group were significantly increased after 6 h (P＜0.05), and the levels in the HCd group were significantly increased after 6 and 24 h (P＜0.05). Cadmium exposure during pregnancy significantly up-regulated HIF-1α and DRP1 expressions and down-regulated the expressions of oxidative phosphorylation-related proteins in maternal livers. In vitro cell experiments showed that the expressions of oxidative phosphorylation-related proteins was significantly decreased and HIF-1α and DRP1 protein expressions were significantly increased in the AML12 cells treated with CdCl2 for 6 h. Mdivi-1 pretreatment significantly antagonized the inhibitory effect of cadmium on the expressions of oxidative phosphorylation-related proteins in AML12 cells, while Hif-1α siRNA pretreatment significantly antagonized the up-regulative effect of cadmium on DRP1 expression in AML12 cells. Conclusion Cadmium exposure in pregnant mice may up-regulate DRP1 expression by activating HIF-1α signaling, then inhibit oxidative phosphorylation level of hepatic cells, and ultimately lead to maternal liver damage.

Design and applications of synthetic electroactive microbial consortia / 生物工程学报

Synthetic electroactive microbial consortia, which include exoelectrogenic and electrotrophic communities, catalyze the exchange of chemical and electrical energy in cascade metabolic reactions among different microbial strains. In comparison to a single strain, a community-based organisation that assigns tasks to multiple strains enables a broader feedstock spectrum, faster bi-directional electron transfer, and greater robustness. Therefore, the electroactive microbial consortia held great promise for a variety of applications such as bioelectricity and biohydrogen production, wastewater treatment, bioremediation, carbon and nitrogen fixation, and synthesis of biofuels, inorganic nanomaterials, and polymers. This review firstly summarized the mechanisms of biotic-abiotic interfacial electron transfer as well as biotic-biotic interspecific electron transfer in synthetic electroactive microbial consortia. This was followed by introducing the network of substance and energy metabolism in a synthetic electroactive microbial consortia designed by using the "division-of-labor" principle. Then, the strategies for engineering synthetic electroactive microbial consortiums were explored, which included intercellular communications optimization and ecological niche optimization. We further discussed the specific applications of synthetic electroactive microbial consortia. For instance, the synthetic exoelectrogenic communities were applied to biomass generation power technology, biophotovoltaics for the generation of renewable energy and the fixation of CO2. Moreover, the synthetic electrotrophic communities were applied to light-driven N2 fixation. Finally, this review prospected future research of the synthetic electroactive microbial consortia.

Engineering microbial consortia through synthetic biology approach / 生物工程学报

There are a large number of natural microbial communities in nature. Different populations inside the consortia expand the performance boundary of a single microbial population through communication and division of labor, reducing the overall metabolic burden and increasing the environmental adaptability. Based on engineering principles, synthetic biology designs or modifies basic functional components, gene circuits, and chassis cells to purposefully reprogram the operational processes of the living cells, achieving rich and controllable biological functions. Introducing this engineering design principle to obtain structurally well-defined synthetic microbial communities can provide ideas for theoretical studies and shed light on versatile applications. This review discussed recent progresses on synthetic microbial consortia with regard to design principles, construction methods and applications, and prospected future perspectives.

Expression and purification of Mycobacterium tuberculosis WhiB2 and analysis of its physicochemical properties and structure based on bioinformatics / 中华微生物学和免疫学杂志

Objective:To investigate the mechanism of Mycobacterium tuberculosis ( Mtb) WhiB2 in the pathogenesis of tuberculosis. Methods:A recombinant vector of pET28a-WhiB2 for heterogeneous expression of WhiB2 was constructed. The target protein WhiB2 and the inclusion bodies were purified. The differences between denatured and non-denatured WhiB2 were analyzed by circular dichroism and nuclear magnetic resonance. Ferrous ion (Fe 2+ ) was used to restore the iron-sulfur cluster of WhiB2. The interaction between WhiB2 and the upstream promoter sequence of the WhiBMtb gene was analyzed by nuclear magnetic resonance. The tertiary structure of WhiB2 and interacting proteins were analyzed and protein structure alignment was performed based on bioinformatics. Results:The structure of the renatured WhiB2 was basically the same as that of the non-denatuous WhiB2. In addition, Fe 2+ could restore the iron-sulfur cluster of WhiB2. It was found that WhiB2 could bind to the upstream promoter sequence of the WhiBMtb/WhiB2Ms gene. Conclusions:Mtb WhiB2 played a key role in the pathogenesis of tuberculosis, which would contribute to future exploration of novel targets against Mtb.

Expression and prognostic significance of cell division cycle associated protein 5 in pancreatic cancer tissues / 肿瘤研究与临床

Objective:To analyze the expression of cell division cycle associated protein 5 (CDCA5) in pancreatic cancer tissues and its correlation with prognosis based on the bioinformatics.Methods:The RNA sequencing data (HTSeq-FPKM) and corresponding clinical information of 168 pancreatic cancer samples from January to December 2021 were downloaded from the Cancer Genome Atlas (TCGA) database, and the data of 179 pancreatic patients from January to December 2021 were downloaded from the GEPIA2 database, and 171 normal pancreatic tissues from TCGA and GTEx databases were simultaneously integrated. The relative expression level of CDCA5 mRNA in pancreatic cancer patients in GEPIA2 database and its relationship with overall survival (OS) and disease-free survival (DFS) were explored. Combined with the clinical data of the patients, univariate and multivariate Cox regression model analysis was used to analyze the factors influencing the OS of pancreatic cancer patients. Gene set enrichment analysis (GSEA) was performed to investigate the possibly involved signal pathways of CDCA5 in pancreatic cancer.Results:In the GEPIA2 database, the relative expression level of CDCA5 mRNA in pancreatic cancer tissues was higher than that in normal pancreatic tissues, and the difference was statistically significant ( P < 0.05). The pancreatic cancer patients were divided into the high CDCA5 mRNA expression group (89 cases) and the low CDCA5 mRNA expression group (89 cases) according to the median of relative expression level of CDCA5 mRNA (the case equal to the median value was not subgrouped). Survival analysis showed that patients with high CDCA5 mRNA expression had shorter OS ( P = 0.024) and DFS ( P = 0.025) compared with those with low CDCA5 mRNA expression. Multivariate Cox analysis showed that in TCGA database, N staging ( HR = 2.15, 95% CI 1.24-3.72, P = 0.006) and CDCA5 expression ( HR = 1.71, 95% CI 1.23-2.38, P = 0.001) were independent influencing factors of OS for pancreatic cancer patients. The results of GSEA enrichment analysis indicated that high CDCA5 mRNA expression was enriched in 13 biological pathways [all P < 0.05, false discovery rate (FDR) < 0.005] including cell cycle, DNA replication, homologous recombination, pyrimidine metabolism, mismatch repair, pentose phosphate pathway, glycolysis gluconeogenesis and p53. The expression of CDCA5 mRNA was positively correlated with the expressions of HK2, PKM, PGK1, ALDOA, EN01 and LDHA (all P < 0.05). Conclusions:CDCA5 is highly expressed in pancreatic cancer tissues and is associated with poor prognosis of patients, and it can be used as a prognostic marker for pancreatic cancer.

Effects of liraglutide on inflammation and mitochondrial fusion/division in Parkinson' s disease model of mice induced by paraquat / 解剖学报

Objective To investigate the protective effect and mechanism of liraglutide on the paraquat (PQ)⁃ induced Parkinson's disease (PD) mouse model. Methods Totally 24 Kunming mice were randomly divided into control group, PQ group and PQ +liraglutide group, 8 mice in each group. PD model was established by intraperitoneal injection of PQ (10 mg/kg) for 5 consecutive days, and liraglutide (50 nmol/kg) was injected intraperitoneally for 7 consecutive days. The free⁃standing and locomotor activity of mice were measured by behavioral method. Immunofluorescence was used to observe the number of tyrosine hydroxylase (TH) and ionized calcium binding adaptor molecule 1 (Iba1) immunoreactive cells. Western blotting was used to detect the expression of protein TH, glial fibrillary acidic protein (GFAP), mitofusin⁃2 (Mfn2) and dynamin⁃related protein 1 (Drp1). Results The numbers of free⁃standing and locomotor activity numbers decreased significantly (P<0.01, P < 0.05) in PQ group compared with the control group, and the number of TH immunoreactive cells and TH protein expression in substantia nigra decreased significantly (P<0.01, P<0.01) compared with the control group, while the number of Iba1 immunoreactive cells and GFAP protein expression increased significantly (P<0.01, P<0.01) compared with the control group; the expression of Drp1 protein in PQ group was significantly higher than that in control group (P<0.05), while the Mfn2 protein expression decreased significantly (P<0.05) compared with the control group. After treatment with liraglutide, the number of TH positive cells in PQ + liraglutide group was significantly lower than that in control group (P<0.05); the numbers of free⁃standing and locomotor activity increased significantly (P<0.05, P<0.05) in PQ + liraglutide group compared with the PQ group, and the number of TH positive cells and expression of TH protein in PQ + liraglutide group were significantly higher than that in PQ group (P<0.01, P< 0.01); while the number of Iba1 positive cells and GFAP protein expression decreased significantly (P<0.01, P<0.05) compared with the PQ group; the Drp1 protein expression decreased significantly (P<0.01) compared with the PQ group, while the expression of Mfn2 protein in PQ + liraglutide group was significantly higher than that in PQ group (P<0.01). Conclusion Liraglutide has neuroprotective effect by reducing neuroinflammation in substantia nigra, regulating mitochondrial fusion and fission.

Biological Functions and Mechanism of Casein KinaseHrr25 / 中国生物化学与分子生物学报

Hrr25 is a member of the casein kinase 1 family in Saccharomyces cerevisiae and has serine / threonine protein kinase activity. It can function by phosphorylating a variety of proteins. The substrate proteins of Hrr25 include autophagy related proteins, COPII (coat protein complexes II) vesicle coat proteins Sec24 and Sec23, eukaryotic translation initiation factor 6, γ- Tubulin tub4, and extender complex protein 1, etc. In addition, Hrr25 can also interact with meiotic recombinant protein Rec8, Nucleoporin Nup53, transcription regulator Crz1, and transcription activator Haa1, etc. A variety of interacting proteins of Hrr25 enable it to play a role in autophagy, vesicular transport, microtubule assembly, meiosis, mitosis, DNA repair, ribosomal biogenesis, weak organic acid stress and other biological processes. In order to better understand the action mechanism of Hrr25 in various biological processes and the relationship between various biological processes, this paper summarizes the biological functions and action mechanism of Hrr25, and the potential significance of its research, so as to provide a theoretical basis for the further research of Hrr25.

Identification of anti-Mycobacterium tuberculosis agents targeting the interaction of bacterial division proteins FtsZ and SepFe

Tuberculosis (TB) is one of the deadly diseases caused by Mycobacterium tuberculosis (Mtb), which presents a significant public health challenge. Treatment of TB relies on the combination of several anti-TB drugs to create shorter and safer regimens. Therefore, new anti-TB agents working by different mechanisms are urgently needed. FtsZ, a tubulin-like protein with GTPase activity, forms a dynamic Z-ring in cell division. Most of FtsZ inhibitors are designed to inhibit GTPase activity. In Mtb, the function of Z-ring is modulated by SepF, a FtsZ binding protein. The FtsZ/SepF interaction is essential for FtsZ bundling and localization at the site of division. Here, we established a yeast two-hybrid based screening system to identify inhibitors of FtsZ/SepF interaction in M. tuberculosis. Using this system, we found compound T0349 showing strong anti-Mtb activity but with low toxicity to other bacteria strains and mice. Moreover, we have demonstrated that T0349 binds specifically to SepF to block FtsZ/SepF interaction by GST pull-down, fluorescence polarization (FP), surface plasmon resonance (SPR) and CRISPRi knockdown assays. Furthermore, T0349 can inhibit bacterial cell division by inducing filamentation and abnormal septum. Our data demonstrated that FtsZ/SepF interaction is a promising anti-TB drug target for identifying agents with novel mechanisms.

CRISPR/Cas9-mediated Knockout of KIFC1 Inhibits Proliferation and Induces Apoptosis of Cervical Cancer Cells / 肿瘤防治研究

Objective To investigate the functions of the KIFC1 gene in tumor cells and its effect on the proliferation of cervical cancer cells. Methods We designed sgRNAs targeting the KIFC1 gene and constructed a recombinant plasmid based on the pSpCas9 (BB)-2A-GFP vector, which was co-transfected into HeLa cells. We screened monoclonal knockout cell lines through flow cytometry sorting, limited dilution inoculation of cells, and sequencing. RT-qPCR, Western blot, and immunofluorescence were used to detect the transcription and protein expression levels of KIFC1 in knockout cells. Cell phenotypes such as nucleus and microtubule cytoskeleton were observed using phase-contrast microscopy and fluorescence confocal microscopy. Cell proliferation, cell cycle, and apoptosis were analyzed by growth curve plotting, EdU labeling, and acridine orange staining. Results The deletion of the KIFC1 gene resulted in the abnormal phenotypes of HeLa cells, with increased numbers of multinuclei, micronucleus, and disordered microtubules. The cell cycle was disrupted, accompanied with a significant increase in the ratio of late apoptotic cells and a decrease in cell proliferation (all P < 0.05). Conclusion KIFC1 gene deletion affects the assembly of microtubules and cell division in HeLa cells, leading to abnormal nuclear morphology, chromatin elimination, cell cycle arrest, and increased cell apoptosis.

Influencing of inferior mediastinum and esophageal hiatus lymph node metastasis in Siewert type Ⅱ adenocarcinoma of esophagogastric junction invading submucosal different venous divisions of esophagogastric junction / 中华消化外科杂志

Objective:To investigate the influencing of inferior mediastinum and esophageal hiatus lymph node metastasis by submucosal different venous divisions of esophagogastric junction (EGJ) invaded in Siewert type Ⅱ adenocarcinoma of esophagogastric junction (AEG).Methods:The retrospective case-control study was conducted. The clinicopathological data of 547 patients with Siewert type Ⅱ AEG who were admitted to Shanxi Cancer Hospital from January 2018 to December 2021 were collected. There were 461 males and 86 females, aged 61 (range, 33?75)years. Observa-tion indicators: (1) lymph node metastasis rate in different groups; (2) influencing factors of inferior mediastinum lymph node metastasis in Siewert type Ⅱ AEG; (3) influencing factors of esophageal hiatus lymph node metastasis in Siewert type Ⅱ AEG. Measurement data with normal distribution were represented as Mean± SD, and comparison between groups was conducted using the t test. Measurement data with skewed distribution were represented as M(range) or M( Q1, Q3), and com-parison between groups was conducted using the Mann-Whitney U test. Count data were described as percentages or absolute numbers, and comparison between groups was conducted using the chi-square test. The univariate analysis was conducted using the corresponding statistical methods based on data type. The Logistic regression model was used for multivariate analysis. Results:(1) Lymph node metastasis rate in different groups. The lymph node metastasis rate in No. 1, 2, 3, 4sa, 4sb, 7, 8a, 9, 11,20, 108, 110 of the 547 patients was 17.37%(95/547), 6.76%(37/547), 46.44%(254/547), 1.65%(9/547), 1.10%(6/547), 23.22%(127/547), 4.39%(24/547), 3.11%(17/547), 3.47%(19/547), 3.66%(20/547), 0.55%(3/547), 4.20%(23/547), respectively. Of the 547 patients, there were 456 cases with proximal of AEG invading submucosal palisade venous of EGJ including 4 cases with inferior mediastinum lymph node metastasis and no case with media mediastinum or up media-stinum lymph node metastasis. There were 91 cases with proximal of AEG invading submucosal perforator venous of EGJ including 18 cases with inferior mediastinum lymph node metastasis, 3 cases with media mediastinum lymph node metastasis and no case with up mediastinum lymph node metastasis. (2) Influencing factors of inferior mediastinum lymph node metastasis in Siewert type Ⅱ AEG. Results of multivariate analysis showed that age, tumor invading submucosal perforator venous of EGJ and histological classification were independent influencing factors for inferior media-stinum lymph node metastasis of Siewert type Ⅱ AEG ( odds ratio=0.93, 23.33, 0.31, 95% confidence interval as 0.87?0.99, 4.18?130.28, 0.12?0.78, P<0.05). (3) Influencing factors of esophageal hiatus lymph node metastasis in Siewert type Ⅱ AEG. Tumor invading submucosal perforator venous of EGJ was an independent influencing factor for esophageal hiatus lymph node metastasis of Siewert type Ⅱ AEG ( odds ratio=14.95, 95% confidence interval as 2.46?90.76, P<0.05). Conclusion:Age, tumor invading submucosal perforator venous of EGJ and histological classification are independent influencing factors for inferior mediastinum lymph node metastasis of Siewert type Ⅱ AEG, and tumor invading submucosal perforator venous of EGJ is an independent influencing factor for esophageal hiatus lymph node metastasis.

Linkage of blood cell division cycle 42 with T helper cells, and their correlation with anxiety, depression, and cognitive impairment in stroke patients

Cell division cycle 42 (CDC42) regulates T helper (Th) cell differentiation and is related to psychological disorders. This study aimed to assess the correlation between blood CDC42 and Th cells, and their association with mental issues in stroke patients. Peripheral blood samples were obtained from 264 stroke patients and 50 controls. Then, serum CDC42 was measured by enzyme-linked immunosorbent assay, and Th1, Th2, and Th17 cells were detected by flow cytometry. Hospital Anxiety and Depression Scale (HADS) and Mini Mental State Examination (MMSE) were applied to patients. CDC42 was decreased (P<0.001), Th1 (P=0.013) and Th17 (P<0.001) cells were elevated, while Th2 cells (P=0.108) showed no difference in stroke patients compared to controls. In addition, CDC42 was negatively associated to Th1 (P=0.013) and Th17 (P<0.001) cells in stroke patients but were not associated with Th2 cells (P=0.223). Interestingly, CDC42 was negatively associated with HADS-anxiety (P<0.001) and HADS-depression scores (P=0.034) and positively associated with MMSE score (P<0.001) in stroke patients. Lower CDC42 was associated to lower occurrence of anxiety (P=0.002), depression (P=0.001), and cognitive impairment (P=0.036) in stroke patients. Furthermore, increased Th17 cells were positively correlated with HADS-anxiety and HADS-depression scores and inversely correlated with MMSE score, which were also associated with higher occurrence of anxiety, depression, and cognitive impairment in stroke patients (all P<0.05). Blood CDC42 and Th17 cells were correlated, and both of them were linked to the risk of anxiety, depression, and cognitive impairment. However, the findings need further large-scale validation, and the implicated mechanism needs more investigation.

El cuidado como cuestión de tiempo: una perspectiva feminista sobre el tiempo cotidiano de cuidadoras de personas adultas con discapacidad / Caregiving as a matter of time: A feminist perspective of the daily time of women caregivers of adults with disabilities / O cuidado como questão de tempo: uma perspectiva feminista sobre o tempo cotidiano de mulheres cuidadoras de pessoas adultas com deficiência

El artículo aborda el carácter histórico y político del tiempo, por considerarle un articulador de la existencia humana, a partir de una lectura crítica de la vida cotidiana. La reproducción de la temporalidad está mediada por el trabajo, el que, analizado desde una perspectiva feminista-marxista, permite reconocer desigualdades en la experiencia del tiempo de hombres y mujeres, basadas en la división sexual del trabajo. Para profundizar en este fenómeno se presentan resultados derivados de una investigación cualitativa que buscó analizar la experiencia del tiempo cotidiano de mujeres que realizan el trabajo de cuidados de personas adultas con discapacidad en Santiago de Chile. Se realizaron entrevistas semiestructuradas y observaciones participantes en los contextos cotidianos de ocho mujeres, reconociendo que su experiencia del tiempo depende de otras personas, que está densificada por la continua realización simultánea de trabajos no remunerados y que no distingue tiempos libres, de ocio ni por fuera del cuidado. Se concluye reconociendo que las desigualdades de género también producen desigualdades en la experiencia del tiempo, lo que insta a avanzar en políticas sociales que reconozcan el cuidado como un derecho social para que las mujeres puedan construir experiencias de sentido, azar, demora y libertad.

The article addresses time's historical and political character, considering it an articulator of human existence from a critical reading of everyday life. The reproduction of temporality is mediated by work, which, analyzed from a feminist-Marxist perspective, allows the recognition of inequalities in the experience of time for men and women based on the sexual division of labor. To delve into this phenomenon, the article presents the results from qualitative research that sought to analyze the daily experience of women who are caregivers of adults with disabilities in Santiago de Chile. Methodologically, semi-structured interviews and participant observations were made in the everyday contexts of eight women, recognizing that their experience of time depends on other people, that it is densified by the continuous and simultaneous performance of unpaid work, and that it does not distinguish free time, leisure, or time outside care. It concludes by recognizing that gender inequalities also produce inequalities in the experience of time, which urges progress in social policies recognizing care as a social right so that women can build experiences of meaning, chance, delay, and freedom.

O artigo aborda a natureza histórica e política do tempo, considerando-o um articulador da existência humana, a partir de uma leitura crítica da vida cotidiana. A reprodução da temporalidade é mediada pelo trabalho, que, analisado a partir de uma perspectiva feminista-marxista, permite reconhecer desigualdades na experiência do tempo de homens e mulheres, a partir da divisão sexual do trabalho. Para aprofundar este fenômeno, são apresentados os resultados derivados de uma pesquisa qualitativa, que buscou analisar a experiência do tempo cotidiano de mulheres que realizam o trabalho de cuidar de adultos com deficiência em Santiago do Chile. Foram realizadas entrevistas semiestruturadas e observações participantes nos contextos cotidianos de oito mulheres, reconhecendo que sua experiência do tempo depende de outras pessoas, que está intensificada pelo desempenho contínuo e simultâneo de trabalhos não remunerados, e que não distingue os tempos livres, de lazer nem externo ao cuidado. Conclui-se reconhecendo que as desigualdades de gênero também produzem desigualdades na experiência do tempo, o que urge avançar em políticas sociais que reconheçam o cuidado como um direito social, para que as mulheres possam construir experiências de sentido, casualidade, lentidão e liberdade.

Embracing the cordillera: Luis Carranza Ayarza and the development of environmental imaginaries in late-nineteenth-century Peru / Abrazando la cordillera: Luis Carranza Ayarza y el desarrollo de los imaginarios ambientales en el Perú de fines del siglo XIX

Abstract This article examines the relationship between local scientific ideas about the natural world and the economic potential to transform the modern nation-state in Peru during the late nineteenth century. Writings by the Peruvian scientist Luis Carranza indicate how support for a distinctive environmental imaginary of the country's geography made it possible to conceptualize nature as an essential component of Peruvian identity. As a result, local scientists had to "imaginatively" reshape the nature of the Andes for modernization purposes. The social and political ramifications of these ideas in Carranza's work were key to the foundation of scientific institutions such as the Geographical Society of Lima.

Resumen Este artículo examina la relación entre las ideas científicas locales sobre el mundo natural y el potencial económico para transformar el Estado-nación moderno en el Perú a fines del siglo XIX. Los escritos del científico peruano Luis Carranza señalan cómo el apoyo a un imaginario ambiental distintivo de la geografía del país permitió conceptualizar la naturaleza como componente esencial de la identidad peruana. Como resultado, los científicos locales tuvieron que remodelar "imaginativamente" la naturaleza de los Andes con fines de modernización. Las ramificaciones sociales y políticas de estas ideas en la obra de Carranza fueron clave para la fundación de instituciones científicas como la Sociedad Geográfica de Lima.

"The one who takes care is the mother": Legal mediators' child care imaginary / "Quem cuida é a mãe": imaginário de mediadores judiciais sobre cuidado infantil

Objective Investigate child care collective imaginaries, a focus justified by the social and family transformations resulting from the massive insertion of women into the labor market. Method The study is organized methodologically from the perspective of concrete psychoanalytic psychology, articulating the use of the psychoanalytic method with relational theorizations. The research involves collective psychological interviews, in which 12 judicial mediators were interviewed through the Thematic Drawing and Story Procedure. Results The psychoanalytical consideration of the material allowed the interpretative production of four affective-emotional meaning fields. Conclusion The general picture indicates that a conservative imaginary prevails in which the best child care is provided by the biological mother in the contexts of both the nuclear family and the matrifocal family while also maintaining a friendly bond with the father.

Objetivo Investigar imaginários coletivos sobre cuidado infantil, justificando-se face a transformações sociais e familiares decorrentes da maciça inserção da mulher no mundo laboral. Método Organizou-se metodologicamente na perspectiva da psicologia psicanalítica concreta, referencial que articula o uso do método psicanalítico com teorizações relacionais. Concretizou-se pela realização de entrevistas psicológicas coletivas, durante as quais 12 mediadores judiciais foram abordados por meio do uso do Procedimento de Desenhos-Estórias com Tema. Resultados A consideração do material permitiu a produção interpretativa de quatro campos de sentido afetivo-emocional. Conclusão O quadro geral indica o predomínio de um imaginário conservador, conforme o qual o melhor cuidado infantil é aquele proporcionado pela mãe biológica no contexto da família nuclear ou da família matrifocal que mantém vínculo amistoso com o pai.