RESUMEN
Objective:To establish the fingerprint of standard decoction of Citri Reticulatae Pericarpium and evaluate its quality. Method:According to the preparation conditions of the standard decoction,15 batches of standard decoction of Citri Reticulatae Pericarpium were prepared.HPLC was employed to determine the content of hesperidin in this standard decoction.Ultraviolet spectroscopy(UV) and infrared spectroscopy(IR) were used to establish the fingerprint of standard decoction of Citri Reticulatae Pericarpium.The correlation coefficient method and double index sequence analysis method were used to compare and analyze the spectra of different batches of this standard decoction. Result:The content of hesperidin in 15 batches of this standard decoction were 0.82%-2.60%,and the measured value of dry extract rate was 32.02%-46.11%.Compared with ultraviolet and infrared control fingerprint,the fingerprint similarities of the standard decoction of each batch were > 0.897 and > 0.942,respectively.The double index analysis results showed that the common peak ratio was more than 62.50%,variation peak ratio was less than 46.67%. Conclusion:The quality evaluation method established in this study can be used for systematic evaluation of standard decoction of Citri Reticulatae Pericarpium,and it can provide theoretical reference for the formulation of quality standard of Citri Reticulatae Pericarpium dispensing granules and other related preparations.
RESUMEN
Objective To prepare 15 batches of Chuanxiong Rhizoma standard decoctions and evaluate its quality. Methods According to preparation conditions of the TCM standard decoction, 15 batches of Chuanxiong Rhizoma standard decoction were prepared; The content of ferulic acid chlorogenic acid and caffeic acid in Chuanxiong Rhizoma standard decoction were determined by using HPLC; The fingerprint of Chuanxiong Rhizoma standard decoction was established by using UV-visible spectrophotometry and infrared spectroscopy; And diverse batches of Chuanxiong Rhizoma standard decoction fingerprint were comparative analyzed by using the correlation coefficient method and double-index analysis method. Results The results of 15 batches of samples revealed that the transfer rate of ferulic acid ranged from 0.485 7% to 0.749 5 %, the transfer rate of chlorogenic acid ranged from 0.316 0% to 0.565 6%, the transfer rate of caffeic acid ranged from 0.017 7% to 0.050 4%, and extract rate was at the range of 18.82%-27.60%. Compared with the UV and IR standard fingerprint, the similarity of Chuanxiong Rhizoma standard decoction fingerprint was more than 0.990 and 0.911 respectively. The results of double-index analysis showed that the common peak rate of each lot of Chuanxiong Rhizoma standard decoction was more than 51.72%. Conclusion This quality evaluation method can be used for the systematic evaluation of Chuanxiong Rhizoma standard decoction. It also provides a theoretical reference preparation for the formulation of quality standard of for the formulation of quality standard for Chuanxiong Rhizoma preparations such as Chuanxiong Rhizoma dispensing granules.