A case -control study on the association between single nucleotide polymorphism of eNOS gene T -786C and G894T and the risk of coronary heart disease / 预防医学

Objective To explore the association between single nucleotide polymorphism(SNPs)of eNOS gene T -786C and G894T and the risk of coronary heart disease (CHD).Methods Genotypes of single nucleotide polymorphism (SNPs)of eNOS gene T -786C and G894T were detected in 842 CHD patients and 842 age and gender matched healthy control individuals using Taqman -polymerase chain reaction genotyping assay and the association between them were analyzed.Results Genotype distributions of the two loci in controls were in consistence with Hardy -Weinberg inheritance model.Frequencies of allele C (8.79% vs.6.53%,P =0.01 4,OR =2.54,95%CI =2.01 -3.1 4),CC (0.84% vs. 0.1 1 %,P =0.04,OR =3.81 ,95%CI =1 .74 -5.56)and TC /CC (1 6.75% vs.1 3.06%,P =0.03,OR =2.1 1 ,95%CI =1 .75 -2.98)genotypes in cases were obviously higher than in controls.However,there was no significant frequency difference of TC genotype in two groups.No significant association was found between allele T,genotype GT,TT and GT/TT and the risk of CHD.Conclusion Allele C,genotype CC and TC /CC of T -786C within eNOS gene were statistically significantly associated with increased risk of CHD,indicating that this locus might be involved in the pathological process of CHD,and allele C,genotype CC,TC /CC might be genetic susceptible factors for CHD.

Effect of L-arginine on the Expression of Endothelial Nitric Oxide Synthase (eNOS) in Heart Tissues of Diabetic Rats.

Aims: This study aimed to determine the role of L-arginine on eNOS expression and its role on the level of some antioxidants as reduced glutathione and catalase in alloxan-induced diabetic rats. Study Design: Ninety adult male albino rats were divided into three (3) groups of thirty rats (30) each: Group (I): Control group, Group (II): Diabetic control group, Group (III): L-arginine treatment group. The second and the third groups were made diabetic by intraperitoneal administration of 80 mg/kg alloxan monohydrate, while L-arginine (100 mg/kg in sterile 0.9% Nacl) was given orally to rats in group III for one week before alloxan injection, and a further four weeks after induction of diabetes. The animals were sacrificed and blood collected for the determination of biochemical and antioxidant markers. Heart tissues were homogenized for determination of expression of eNOS gene by RT- PCR. Results: The study showed that L-arginine increases HDL, reduces glutathione and catalase and decreases LDL, TAG, Cholesterol and MDA. It also increased the expression of eNOS in heart tissues of diabetic rats.

eNOS3 Genetic Polymorphism Is Related to Post-Ablation Early Recurrence of Atrial Fibrillation

PURPOSE: Previous studies have demonstrated an association between eNOS polymorphisms and atrial fibrillation (AF). We sought to determine whether eNOS polymorphisms are associated with AF recurrence after a radiofrequency catheter ablation (RFCA). MATERIALS AND METHODS: A total of 500 consecutive patients (56+/-11 years, 77% male) with paroxysmal (68%) or persistent (32%) AF who underwent RFCA and 500 age, gender-matched controls were genotyped for the eNOS3 single nucleotide polymorphism (rs1799983). AF recurrence was monitored according to 2012 ACC/AHA/ESC guidelines. RESULTS: The frequencies of the rs1799983 variant alleles (T) in the case and control group were not significantly different (OR 1.05, 95% CI 0.75-1.46, p=0.798). AF patients with rs1799983 variants were more likely to have coronary artery disease or stroke than those without genetic variant at this gene (31.0% vs. 17.3%, p=0.004). During mean 17 months follow-up, early recurrence of AF (ERAF; within 3 months) and clinical recurrence (CR) of AF were 31.8% and 24.8%, respectively. The rs1799983 variant was associated with higher risk of ERAF (OR 1.71, 95% CI 1.06-2.79, p=0.028), but not with CR. ERAF occurred earlier (11+/-16 days) in variant group than those without variant allele (20+/-25 days, p=0.016). A multiple logistic regression analysis showed that presence of the rs1799983 variant (OR 1.75, 95% CI 1.07-2.86, p=0.026) and persistent AF were independent predictors for ERAF after AF ablation. CONCLUSION: The rs1799983 variant of the eNOS3 gene was associated with ERAF, but not with CR, after RFCA. eNOS3 gene variants may have a potential role for stratification of post-ablation management.

Effect of health management on morbidity risk of hypertension in population with eNOS gene G894T mutation / 心血管康复医学杂志

Objective:To explore the effect of health management on morbidity risk of hypertension in population with endotheliocyte nitric oxide synthetase (eNOS)gene G894T mutation.Methods:A total of 360 cases,who were 45~60 years old and possessed eNOS gene G894T mutation but without hypertension,were screened using gene chips detection from people who received physical examination and recreation in Qingdao sanatorium and patients visiting to affiliated hospital of medical college of Qingdao University from Jun 2010 to Jun 2013. According to their compli-ance condition,they were divided into health management group (n=163)and control group (n=197,continued their intrinsical lifestyle).Both groups were followed up for two years,and their incidence rates of hypertension were compared.Results:After two-year follow-up,a total of 17 cases (10.43%)were diagnosed as hypertension a-mong 163 cases of health management group;it was significantly lower than that in control group (24.87%,χ2 =12.43,P =0.001).Conclusion:Scientific health management can effectively reduce incidence rate of hypertension in population with eNOS gene G894T mutation.

Association between four SNPs of eNOS gene and EH in Ningxia Hui population * / 重庆医学

Objective To investigate the correlation between 4 SNPs of eNOS gene and EH in Ningxia Hui population ,and to provide theory basis for taking precautions against .Methods Polymerase chain reaction and restriction fragment length polymor-phism(PCR-RFLP) genotyping method were carried out to examine SNPs at rs2070744(T>C) ,rs1799983(G> T) ,rs1800780(A>G) and rs3918181(A>G)sites of eNOS gene ,including 134 EH patients and 115 healthy individuals in Ningxia Hui population .For EH patient-control haplotype analyses ,SHEsis online haplotype analysis software was applied .Results The genotypic frequencies of rs1800780 and rs1799983 had significant difference between patients group and normal group (P<0 .05);The allele frequency of rs1799983 had significant difference between patients group and normal group(P<0 .05);and the frequency of G allele in EH group was less than healthy controls ,which OR value was 3 .851(95% CI:2 .236 -6 .631) .According to the four SNP sites 15 kinds of haplotype were detected ,of which haplotype CGAG ,TTAG ,TGGG ,TTGG ,TTGA in Ningxia Hui healthy people and in patients with EH had a statistics difference(P<0 .05) .haplotype CGAG ,TGGG OR of 0 .352 ,0 .600 ,95% CI less than 1;haplotype TTAG OR 2 .689 ,95% CI greater than 1 .Conclusion haplotype with the emergence of CGAG ,TGGG may reduce the risk of EH Hui na-tionality .haplotype the emergence of TTAG may increase the risk of EH Hui nationality .

Prevalence of endothelial nitric oxide synthase (eNOS) gene exon 7 Glu298Asp variant in North Eastern India.

Background & objectives Endothelial nitric oxide is a potent vasodilator and impairment of its generation brought about by gene polymorphism is considered a major predictor for several diseases. A single nucleotide polymorphism G894T within exon 7 of endothelial nitric oxide synthase (eNOS-7) gene, resulting in a replacement of glutamic acid by aspartic acid, has been studied as a putative candidate gene for cardiovascular diseases. The pattern of eNOS-7 Glu298Asp variant in the Indian population is poorly known. The present study was planned to determine the prevalence of the variant of this gene among tea garden community in Assam, North-East India with high prevalence of hypertension. Methods Study participants of both sex aged ≥18 yr were recruited randomly from temporary field clinics established in tea gardens of Dibrugarh, Assam. Genomic DNA was extracted from 409 subjects by the conventional phenol-chloroform method. The prevalence of the eNOS exon 7 Glu298Asp variant was determined by polymerase chain reaction and restriction fragment length polymorphism analysis. Results The study population was in Hardy-Weinberg Equilibrium. The frequency of the eNOS GG, GT and TT genotypes was found to be 75, 22 and 3 per cent respectively and did not show any significant difference in gender wise analysis. Interpretation & conclusions Our results showed that the prevalence of the homozygous GG genotype was high (75%) and the rare mutant genotype (homozygous, TT) was 3 per cent in a population at risk with cardiovascular disease. Such population-based data on various polymorphisms can ultimately be exploited in pharmacogenomics.