RESUMEN
Aim To predict the potential targets of Salvia miltiorrhiza- Kushen herb pairs extracts (SK) and explore its anti-inflammatory effect on modelsbased on network pharmacology, so as to provide the research foundation of both new drugs and anti-inflammatory mechanism. Methods Network pharmacology was used to predict the potential anti-inflammatory targets of SK. Ear edema model was used to study the anti-in- flammatoiy effects of SK. Luminex liquid-phase chip analysis technology was used to observethe changes in secretion of pro-inflammatory cytokines in peripheral serumafter transdermal administration in mice by SK. Intraperitoneal injection of Evans blue experiment was used to simulate the exudation of inflammatory factors, and the effect of SK on capillary permeability in mice- was explored. Results Network pharmacology was used to predict that the anti-inflammatory effect of SK- was mainly related to immune-related processes and VEGF signaling pathways, unravelingthe relationship between the anti-inflammatory mechanismand the decrease of pro-inflammatory factors and vascular permea- bility. Conclusions The experiments verify that the results are the same as the prediction by network pharmacology. The anti-inflammatory effect involves decline the IL-6 levels, granulocyte colony-stimulating factor( G-CSF) levels and vascular permeability in the STAT3 pathway. Asthemain components of SK, tanshi- none, matrine and oxymatrine are linked to anti-in- flammatory effects.
RESUMEN
Sesquiterpene-quinone is a class of secondary metabolites frequently encountered from marine sponge. The present study was designed to examine the anti-inflammatory action of sponge-derived dactyloquinone B (DQB) and cyclospongiaquinone-1 (CSQ1) mixture using lipopolysaccharide (LPS)-induced inflammatory responses. We measured the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein. TNF-alpha, IL-1beta, and IL-6 production, which increased by treatment with LPS, were significantly inhibited by DQB and CSQ1 mixture. It also decreased the production of NO production, and iNOS and COX-2 expression. Furthermore, it reduced 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ear edema of ICR mice. These results demonstrate that sesquiterpene-quinone, DQB and CSQ1 mixture, might serve as a chemical pipeline for the development of anti-inflammatory agent.