RESUMEN
Objective To evaluate the probability of siblings identification in Identifiler system by using the software of automatic analysis.Methods Using the software of automatic analysis in siblings jdentification.STP genetic typing of 151 pairs of full siblings and 31224 pairs of unrelated individuals from manual simulation were analyzed in 15 STR loci of ldentifiler system.Results Kin probability(W_(FS))of 39.07% full siblings were more than 99.999% while W_(FS) of unrelated individual pairs were 0% .W_(FS) of 60.93% full siblings and 21.3% unrelated individual pairs were all at the range from 99.999% to 1% .W_(FS) of 78.7% unrelated individual pairs 0% full siblings individuals were less than 1% .Therefore,there were notability difference between full siblings and unrelated individual pairs.In addition,testing of 15 STR loci of Identifiler system,it suggested that the pair were siblings when the locus number of the entirely-same is not less than 5 or that of the entirely-different is not more than 1,and that the pair were unrelated individuals when the locus number of the entirely-different is not less than 6 or that of the entirely-8alne is not more than 1.Conclusion The software of automatic analysis and the Identifiler system call be used to siblings identification.
RESUMEN
Objective To discuss the probability of siblings identification by autosomal short tandem repeat (STR). Methods 150 pairs of full siblings and 150 pairs of unrelated individuals were genotyped by the 15 STR loci of Power Plex?6 system. Paternity index of full siblings (Pips) and paternity probability of full siblings (WFS) were calculated with the method of ITO. WFS and allelic matching of the two groups were compared, and three sorts of allelic matching in each pair were tested by chi-square test. Results Wps of 100 pairs of siblings (66.67%) were more than 0.9995; WFS of unrelated individuals were less than 0.8, and that of 100 pairs (66.67%) were less than 0.27. The locus number of the entire-same ranged from 1 to 10, averaged 5.49 in siblings, while in unrelated individuals ranged from 0 to 5 and averaged 1.33; that of the entire-different ranged from 0 to 6, averaged 1.66 in siblings but ranged from 2 to 11 and averaged 6.57 in unrelated individuals; that of the half-same ranged from 3 to 13, averaged 7.85 in siblings, and ranged from 1 to 13, averaged 7.11 in unrelated individuals. By chi-square test, there was significant difference (P0.05) in the half-same between the two groups. Conclusion It is effective to identify siblings by Power-Plex?6 system. Testing of 15 STR loci of PowerPlex?6 system, it suggested that the pair were unrelated individuals when the locus number of the entirely-different is not less than 6 or that of the entirely-same is zero, and that the pair were siblings when the locus number of entirely-different is not more than 1 or that of the entirely-same is not less than 6.