Bioanalytical HPLC method of Piper betle L. for quantifying phenolic compound, water-soluble vitamin, and essential oil in five different solvent extracts

A reversed-phase high-performance liquid chromatography with diode-array detection (HPLC-DAD) was developedand validated to estimate the phenolic acids (gallic acid, caffeic acid, syringic acid, p-coumaric acid, sinapic acid, andferrulic acid), flavonoids (catechin rutin, myricetin, quercetin, apigenin, and kaempferol), ascorbic acid, and eugenol.The chromatogram condition was set in suitable wavelength 272 nm and run flow rate 0.7 µl/minutes using HPLCAgilent Technologies 1260 Infinity, a reversed-phase Zorbax SB-C18 column (3.5 µm particle size, i.d. 4.6 mm × 250mm) with the mobile phase solution (1:9, HPLC-grade acetonitrile:1% acetic acid). The linearity, precision, limit ofdetection, limit of detection, and accuracy were R2 > 0.9907, relative standard deviation < 1%, 0.005 µg/ml, 0.015 µg/ml, and 96%–102%, respectively. As a result, all the selective compounds were successfully separated, identified, andquantified. The enormous contents were found in quercetin and eugenol, expressing crude content (mean, 5.989 mg/g)and residue content (mean, 1.934 mg/g) for quercetin, while crude content (mean, 3.209 mg/g) and residue content(mean, 0.184 mg/g) for eugenol. Consequently, this method could be applied, repeated, and developed for the laterobservation, especially in commercially inclination of Piper betle analysis

Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India.

A reversed-phase high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of ascorbic acid, free phenolic acids and flavonoids (catechin, rutin, quercetin, myrecetin, apigenin and Kaempferol) in four different solvent extracts of two wild edible leaves of viz. Sonchus arvensis and Oenanthe linearis, collected from North-eastern region in India . The chromatographic separation was carried out on Acclaim C 18 column (5 μm particle size,250 x 4.6 mm), Dionex Ultimate 3000 liquid chromatograph and detection was carried out at three different wave lengths (272, 280 and 310 nm) using a mobile phase of acetonitrile and 1% aqueous acetic acid solution with gradient elution. The experimental results showed high amount of ascorbic acid in S. arvensis and O. Linearis (1.2% and 2.3 % respectively) and gallic acid (0.02% and 0.06% respectively) in 1% aq. acetic acid extract of these two plants. The high percentage of recovery (96-103%), low coefficient of variation ( R2 > 0.99) and low limit of detection (LOD) and limit of quantitation (LOQ) confirm the suitability of the method for simultaneous quantification of ascorbic acid and all phenolic compounds in the two plants under investigation.