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Objective T o observe cardiac ultrastructure and the expression of heat shock protein 70 (H SP70) and hypoxia inducible factor-1α (H IF-1α) in electric shock death rats and to explore the application of these indexes as the basis of m edical identification in electric shock death. Methods Seventy-tw o SD rats w ere random ly divided into electric shock death group, postm ortem electric shock group and the control group. T he changes of m yocardial ultrastructure w ere observed by transm ission electron m icro-scope, and the expressions of m yocardial H SP70 and H IF-1α w ere observed by im m unohistochem ical technology. Results M yocardial m yofibril fracture, m itochondrial cristae and m em brane dissolution, and disordered arrangem ent of Z lines and M lines w ere observed in electric shock rats. H SP70 and H IF-1αw ere strong positive expressions in the electric shock death group, significantly com pared w ith the con-trol and postm ortem electric shock groups (P<0.05). Conclusion T he expressions of H SP70 and H IF-1αw ere obviously increased in electric shock death group, w hich m ay be used as the diagnostic indicator of electric shock death.
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Heat shock proteins (HSPs) are present in all organisms from E. coli to humans and have been preserved very well over species. HSPs are then found to be induced by various types of stress, not only by heat stress, and repair the stress-induced misfolded proteins, thus protecting the body from stress. HSPs come in various sizes according to their molecular weights, of which HSP70 (70 kDa) is especially well known for being induced by heat stress. HSP70 is efficiently induced by mild hyperthermia and has the important physiologic function of biophylaxis action (protection from stresses), an immunoenhancing effect (activation of NK cells), and a molecular chaperone action (folding of proteins).<BR> We confirmed that HSP70 was induced by mild hyperthermic living (Kaon Seikatsu) such as bath and hot-spring, and established the HSP bathing method (that had taken a bath for 10-20 min. at 40-42°C, keep it warm for 15 minutes after that) to increase HSP70. In the HSP bathing method, HSP70 and NK cells activity increased significantly on two days after bathing. About five minutes could be shortened by using bath additive including the carbonic acid at the bathing time. The rise of the heartbeats with the step going up and down was decreased by HSP bathing, and the physical index increased significantly. In the results of the questionnaire concerning health, tiredness and muscular pain were reduced, the confusion of feelings was controlled, and the result of the improvement mentally and physically was obtained in the HSP bathing.<BR> On the other hand, various functions of whole body decrease with aging. <BR> Also, the induction of HSP70 rapidly decrease on the sixties, the senior citizen become weakening to the stresses and the immunity. In this report, it was shown about the relationship between the benefits of bathing, hot springs and HSP70.<BR> Japan is the best country of long life expectancy in the world, and a lot of Japanese like bath. It seems that one of the reasons of a Japanese long life is the induction of HSP70 by a certain daily activity like bathing, and this would be helpful in reducing medical expenses in the elderly.<BR> Moreover, there are some reports that HSP70 is related to long life. As for recent high-profile folding diseases caused by misfolded proteins, such as Alzheimer disease, and Parkinson disease, HSP70 induced by bathing can be expected to be supplementary remedy through the molecular chaperone effect of HSP70. Especially, bath and hot-spring (40-42°C) in Japan is suitable for inducing HSP70. It seems that the hot-spring hopping is the most reasonable ecological way to keep fit as the healthy method in the old age.
RESUMEN
Heat shock proteins (HSPs) are present in all organisms from E. coli to humans and have been preserved very well over species. HSPs are then found to be induced by various types of stress, not only by heat stress, and repair the stress-induced misfolded proteins, thus protecting the body from stress. HSPs come in various sizes according to their molecular weights, of which HSP70 (70 kDa) is especially well known for being induced by heat stress. HSP70 is efficiently induced by mild hyperthermia and has the important physiologic function of biophylaxis action (protection from stresses), an immunoenhancing effect (activation of NK cells), and a molecular chaperone action (folding of proteins). We confirmed that HSP70 was induced by mild hyperthermic living (Kaon Seikatsu) such as bath and hot-spring, and established the HSP bathing method (that had taken a bath for 10-20 min. at 40-42°C, keep it warm for 15 minutes after that) to increase HSP70. In the HSP bathing method, HSP70 and NK cells activity increased significantly on two days after bathing. About five minutes could be shortened by using bath additive including the carbonic acid at the bathing time. The rise of the heartbeats with the step going up and down was decreased by HSP bathing, and the physical index increased significantly. In the results of the questionnaire concerning health, tiredness and muscular pain were reduced, the confusion of feelings was controlled, and the result of the improvement mentally and physically was obtained in the HSP bathing. On the other hand, various functions of whole body decrease with aging. Also, the induction of HSP70 rapidly decrease on the sixties, the senior citizen become weakening to the stresses and the immunity. In this report, it was shown about the relationship between the benefits of bathing, hot springs and HSP70. Japan is the best country of long life expectancy in the world, and a lot of Japanese like bath. It seems that one of the reasons of a Japanese long life is the induction of HSP70 by a certain daily activity like bathing, and this would be helpful in reducing medical expenses in the elderly. Moreover, there are some reports that HSP70 is related to long life. As for recent high-profile folding diseases caused by misfolded proteins, such as Alzheimer disease, and Parkinson disease, HSP70 induced by bathing can be expected to be supplementary remedy through the molecular chaperone effect of HSP70. Especially, bath and hot-spring (40-42°C) in Japan is suitable for inducing HSP70. It seems that the hot-spring hopping is the most reasonable ecological way to keep fit as the healthy method in the old age.
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Heat shock protein 70 is a molecular chaperone which expressed during oxidative stress to protect the cell from damage. Preeclampsia is a hypertensive disorder with an oxidative stress imbalance. Antioxidant defenses appear to be depleted during preeclampsia resulting in increased oxidative stress. Various alternative medicines are employed to increase the endogenous antioxidant level during preeclampsia. The aim of the present study was to determine the modulatory effect of medicinal plants, Camellia sinensis and Mentha spicata on oxidative stress, antioxidant and thiol status using placental explant as the model system. Placental explants were cultured from the placental tissues of both normotensive and preeclamptic subjects. The lipid peroxide, total antioxidant capacity, glutathione redox ratio, HSP70 levels were measured in the placental explants with and without incubation with tea, mint and mint-tea. The addition of these three extracts increased the TAC and GRR in both placental explants with decrease in the LPO level. The expression of HSP70 also decreased more significantly in preeclamptic explants on addition of tea and mint extracts due to the restoration of cell homeostasis via maintaining the antioxidant status. In view of the above results, mint-tea may emerge as an effective antioxidant, preventing cell damage during stress condition.
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@#ObjectiveTo investigate the effects of Yinhuang solution on the bladder mucosal before and/or after mucosal injury.Methods32 SPF female SD rats were randomly divided into 4 groups with 8 rats in each group. Group A was used for the normal control group, group B for the model control group, group C for preventive group, group D for preventive and therapeutic group. Injured rats were created by hydrochloric acid applied on bladder mucosa. After the models were established or before and after the models were established, bladders were perused with Yinhuang solution. Severity of inflammatory infiltration were observed by HE staining, histological score and heat shock protein 70 (HSP70) expressed in the mucosa.ResultsEndoscopic pathological change showed that group B had a large number of inflammatory cell infiltration, significant expansion of capillary, epithelial cell necrosis. Group C had a small amount of expansion of capillary, inflammatory cell infiltration. Group D scattered in expansion of capillary, a small amount of inflammatory cell infiltration. Concerning the results of histological score and HSP70 expression, there was a significant difference between group A and groups B, C or D (P<0.01); there was a significant difference between group B and groups C or D (P<0.01); there was a significant difference between group C and group D (P<0.05).ConclusionYinhuang solution can evidently improve the repair capability of bladder mucosa after injury.
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Objective:To study its effect on the expression of BDNF from injured astrocytes after HSP70 were blocked by HSP70-antibody. Methods:We performed scratch injuries in the cultured rat astrocytes,and added HSP70-antibody into the nu- trient medium at once after injury(intervention group).Then,at 30min,24h,4d,7d after injury,the expression of brain-derived neurotropic facto(rBDNF)was studied by immunocytochemical and reverse transcriptase-polymerase chain reaction(RT-PCR).The immunocytochemical pictures were analyzed by image analysis system to abtain the average optical density of BDNF. Results: The expression of BDNF increased gradually with injury time.Compared with the control group,the average optical density of BDNF in the intervention group were significantly reduced(P
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It had been proved by many evidences that several heat shock proteins (HSPs) expression is up-regulated in tissue-derived primary lung cancer, and HSPs may play important roles in development, in resistant to drugs and in prognosis of lung cancer. However, there have not still systemic research on which HSPs,especially HSP70 can be or not thought as a new biological target in the therapy to lung cancer. In order to address the expression and roles of heat shock protein 70 (HSP70) in lung adenocarcinoma, immunoblotting was performed to detect the expression of HSP70 in tissue specimens from lung adenocarcinoma which were diagnosed unambiguously by branch fibromicroscopy and were excised. It showed that in the normal lung tissues, the expression of HSP70 was less then that in cancer tissues. After down-regulation of HSP70 protein by HSP70 anti-sense oligonucleotides in A549 cell line, MTT assay showed that the proliferation of A549 cells was inhibited remarkably after the treatment with HSP70 antisense oligonucleotides and Act D. There had significant differential in HSP70 antisense treatment group followed by Act D treatment and Act D treatment group. Results of Hoechst33258 staining revealed that HSP70 antisense oligonucleotides could promote Act D-mediated apoptosis in A549 cells with a higher percentage of apoptotic cells (26.91?3.73)% than that of Act D-treated group (16.83?3.41)% (P
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OBJECT: The intracellular action of the antidepressant, venlafaxine, was studied in C6-gliomas using heat shock protein 70(HSP70) immunocytochemistry and HSP70 Western blots because HSP70 is associated with stress and depression. METHODS: To examine how the glucocorticoid affects the expression of HSP70 in nerve cells, the rat C6 glioma cell was treated with dexamethasone for 6 hours. In addition, venlafaxine was administered to the experimental groups of C6 glioma cells for 1, 6, 24, and 72 hours each, after which the expression of HSP70 was investigated. Finally, venlafaxine and dexamethasone were simultaneously administered to the experimental groups for 1, 6, 24, and 72 hours, followed by an investigation of the expression of HSP70. RESULTS: The short term(1 hour) venlafaxine treatment significantly increased the level of HSP70 expression. The short term treatment of venlafaxine with dexamethasone also increased the level of HSP70 expression but this reduction was not statistically significant. The long term(72 hours) venlafaxine with dexamethasone treatment significantly reduced the level of HSP70 expression. The long term treatment of venlafaxine also reduced the level of HSP70 expression but this reduction was not statistically significant. Dexamethasone(10uM, 6hours) did not affect the level of HSP70 expression compared with controls. CONCLUSION: Venlafaxine increases the expression of HSP70 at short term treatment, but prolonged treatment with dexamethasone suppresses the expression of HSP70.
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Animales , Ratas , Western Blotting , Depresión , Dexametasona , Glioma , Proteínas de Choque Térmico , Inmunohistoquímica , Neuronas , Clorhidrato de VenlafaxinaRESUMEN
OBJECTIVE: The aim of this study was to examine the effect of dexamethasone and fluoxetine on the expression of 70 kDa heat shock protein (HSP70) in C6 glioma cells. METHODS: The C6 glioma cells belong to control group were incubated with DMEM culture solution, the cells belong to dexamethasone group were incubated with dexamethasone for 6 hours, and the cells belong to fluoxetine group were incubated with fluoxetine for 1, 6, 24, and 72 hours, separately, and then exposed to dexamethasone for an additional 6 hours. Crude extracts from control, dexamethasone and fluoxetine-treated C6 glioma cells were separated on a 10% SDS-PAGE and probed with anti-HSP70 mAb. RESULTS: 1) Dexamethasone (10 uM, 6 hours) reduced the level of HSP70 expression relative to control, but this reduction was not statistically significant. 2) Pretreatment with fluoxetine (10 uM, 1, 6, 24, and 72 hours) and exposure to dexamethasone (10 uM, 6 hours) decreased the level of HSP70 expression according to the duration of fluoxetine treatment. 3) Fluoxetine significantly reduced the level of HSP70 at 24 and 72 hours compared to control. However, compare to the level of HSP70 expression at 24 hours, the level of HSP70 expression at 72 hours was elevated. CONCLUSION: These findings suggest that dexamethasone and fluoxetine may affect HSP70 expression through effects on GR.
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Animales , Ratas , Mezclas Complejas , Dexametasona , Electroforesis en Gel de Poliacrilamida , Fluoxetina , Glioma , Proteínas de Choque Térmico , Calor , Proteínas HSP70 de Choque TérmicoRESUMEN
OBJECTIVE: The aim of this study was to examine the effect of dexamethasone and fluoxetine on the expression of 70 kDa heat shock protein (HSP70) in C6 glioma cells. METHODS: The C6 glioma cells belong to control group were incubated with DMEM culture solution, the cells belong to dexamethasone group were incubated with dexamethasone for 6 hours, and the cells belong to fluoxetine group were incubated with fluoxetine for 1, 6, 24, and 72 hours, separately, and then exposed to dexamethasone for an additional 6 hours. Crude extracts from control, dexamethasone and fluoxetine-treated C6 glioma cells were separated on a 10% SDS-PAGE and probed with anti-HSP70 mAb. RESULTS: 1) Dexamethasone (10 uM, 6 hours) reduced the level of HSP70 expression relative to control, but this reduction was not statistically significant. 2) Pretreatment with fluoxetine (10 uM, 1, 6, 24, and 72 hours) and exposure to dexamethasone (10 uM, 6 hours) decreased the level of HSP70 expression according to the duration of fluoxetine treatment. 3) Fluoxetine significantly reduced the level of HSP70 at 24 and 72 hours compared to control. However, compare to the level of HSP70 expression at 24 hours, the level of HSP70 expression at 72 hours was elevated. CONCLUSION: These findings suggest that dexamethasone and fluoxetine may affect HSP70 expression through effects on GR.