Hepatitis C virus core protein effects on expression of mutation p53 and Bcl-2 protein in hepatitis C virus hepatitis and cirrhosis / 医学研究生学报

Objective: To explore the expression of HCV core,mutation p53,and Bcl-2 proteins,and the correlation of these three proteins in the tissues of HCV infection or cirrhosis;to explore weather HCV core protein promotes the production of mutation p53 and/or the expression of Bcl-2 protein. Methods: Collect tissues from 23 HCV infected patients and determine the expression of HCV core,p53,and(Bcl-2) proteins with an immunohistochemical method(Envision method);analyze the correlation of the three proteins by statistics. Results :The positive expression of HCV core and mutation p53 proteins(primarily) lay in the nucleus,while the positive expression of Bcl-2 protein lay in the cytoplasm;the positive rate of mutation p53 was 87.0%,while the positive rate of Bcl-2 was 95.7% in the tissues with HCV core positive expression.There was no difference(P= 0.095) in expression of the three proteins;P value of correlationship test of positive intensity between HCV core and p53,HCV core and Bcl-2 was respectively 0.011 and 0.012,while the correlation coefficient was respectively 0.69 and 0.72;and P value of correlation test of positive intensity between mutation p53 and Bcl-2 was 0.007 with a correlation coefficient as 0.72. Conclusion:The expression of the three proteins is correlated: maybe the HCV core protein promotes mutation of wild p53; HCV core and mutation p53 proteins increase the expression of Bcl-2 protein.

Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library / 第三军医大学学报

Objective To screen and identify proteins that interact with the hepatitis C virus core protein by means of T7-phage display system. Methods The hepatitis C virus core protein was expressed by prokaryotic expression and used as selected molecule to biopan the T7 human liver cDNA library. The selected positive clones were identified by DNA sequence and analyzed with BLAST program in GenBank. Results After BLAST in all positive clones, one protein--Smad interacting protein 1 (SIP1) was found to interact with the hepatitis C virus core protein. Conclusion T7-phage display system is a convenient, rapid and effective method for screening interacting proteins.