Clinical Analysis of Eight Cases of Pemphigus with High Titers of Anti-desmoglein Antibodies in Remission / 中国医学科学院学报

Objective To investigate the clinical manifestations,diagnosis,treatment,and laboratory examination characteristics of 8 pemphigus patients with high titers of anti-desmoglein antibodies in remission. Methods A retrospective study was conducted for the pemphigus patients diagnosed and treated in the department of dermatology from January 2013 to September 2020.The patients should have the serum anti-desmoglein antibodies ≥150 U/ml in remission or the antibody levels dropped less than 20%(calculated based on the maximum detection limit of 150 U/ml)of their initial ones detected before treatment,and the clinical and laboratory data of patients eligible for the inclusion criteria were collected. Results Among the 134 pemphigus patients with available follow-up data during this period,a total of 8 patients met the criteria,with the follow-up period of 21-85 months and the remission duration of 18-70 months.They all received less than or equal to 10 mg/d prednisone and had high titers of anti-desmoglein antibodies.At their first visit,the number of patients with positive anti-desmoglein 1/desmoglein 3 antibodies was 7.Two patients still had high titers of anti-desmoglein 1 antibodies 19 months and 21 months after they achieved remission,and 5 patients had high titers of anti-desmoglein 3 antibodies in 18-70 months.There was one patient showing high titers of both antibodies,especially for anti-desmoglein 1 antibodies.This patient relapsed after 19 months' remission while other patients were still in clinical remission. Conclusions Some pemphigus patients showed persistent high titers of anti-desmoglein antibodies in remission.Anti-desmoglein 3 antibodies were more common to keep positive,while high titer of anti-desmoglein 1 antibodies was less observed.The high titer of anti-desmoglein 1 antibodies had a correlation with recurrence.For the pemphigus patients with long-term clinical remission but high antibody titer,the dosages of corticosteroids should be adjusted carefully according to their actual clinical manifestations and the positive antibody type.For the patients with high titer of anti-desmoglein 1 antibodies,the dosage reduction of corticosteroids should be appropriately slower.

Effects of high titer cold lectin on positive and negative typing of ABO blood group and electron cross matching test / 国际检验医学杂志

Objective To study effect of high titer cold lectin on positive and negative typing of ABO blood group and electron cross matching test .Methods A total of 500 patients needed emergency blood transfusion were selected from November 2015 to October 2016 in People′s Hospital of Qinbei District .To observe the results of electron crossover and serum cross primary and sec-ondary matching ,ABO blood group positive and negative and electronic cross matching experimental results at different tempera-tures ,cold agglutinin test erythrocyte agglutination and cross matching results .Results There was no significant difference on the main side between the electronic cross matching and serological cross matching (P>0 .05) ,but the difference was significant on sec-ondary side(P<0 .05) .In electron cross test ,18 cases appeared agglutination at 20 ℃ ,which was obviously more than that at 37 ℃(P<0 .05) .Patients blood in the positive stereotypes A type ,anti stereotypes B type ,O type all appeared agglutination ,and at 20 ℃O type and self control also appeared agglutination .Cold agglutinin test results showed that when the temperature was lower than 31 ℃ ,red cell agglutination occurred ,when the temperature was lower than 4 ℃ ,visible agglutination ,but when the temperature rose to 37 ℃ ,agglutination disappeared .Conclusion High titer cold lectin could promote the aggregation of red blood cell at low temperature conditions ,and effect the results of ABO blood group positive and negative and electronic cross matching test ,then effect transfusion therapy .

Effects of high titer cold lectin on positive and negative typing of ABO blood group and electron cross matching test / 国际检验医学杂志

Objective To study effect of high titer cold lectin on positive and negative typing of ABO blood group and electron cross matching test .Methods A total of 500 patients needed emergency blood transfusion were selected from November 2015 to October 2016 in People′s Hospital of Qinbei District .To observe the results of electron crossover and serum cross primary and sec-ondary matching ,ABO blood group positive and negative and electronic cross matching experimental results at different tempera-tures ,cold agglutinin test erythrocyte agglutination and cross matching results .Results There was no significant difference on the main side between the electronic cross matching and serological cross matching (P>0 .05) ,but the difference was significant on sec-ondary side(P<0 .05) .In electron cross test ,18 cases appeared agglutination at 20 ℃ ,which was obviously more than that at 37 ℃(P<0 .05) .Patients blood in the positive stereotypes A type ,anti stereotypes B type ,O type all appeared agglutination ,and at 20 ℃O type and self control also appeared agglutination .Cold agglutinin test results showed that when the temperature was lower than 31 ℃ ,red cell agglutination occurred ,when the temperature was lower than 4 ℃ ,visible agglutination ,but when the temperature rose to 37 ℃ ,agglutination disappeared .Conclusion High titer cold lectin could promote the aggregation of red blood cell at low temperature conditions ,and effect the results of ABO blood group positive and negative and electronic cross matching test ,then effect transfusion therapy .

A Case of Anti-JMH Antibody with High-Titer, Low-Avidity Characteristics: The First Case in Korea / 대한수혈학회지

Anti-John Milton Hagen (JMH) is a high-titer, low-avidity (HTLA) antibody against the high frequency red blood cell (RBC) antigen JMH. It occurs very rarely and has not yet been reported in Korea. Here, we report a case of anti-JMH antibody identified in a 92-year-old man without previous blood transfusion history, who had been hospitalized with pneumonia. The patient's hemoglobin level was reduced to 7.6 g/dL on the 35th day of hospitalization, requiring RBC transfusion. Antibody identification test revealed antibodies that showed pan-reactivity to all panel cells at the antiglobulin phase. A titration test confirmed that it was a HTLA antibody. He was given one least-incompatible unit of RBC without any adverse events, and his hemoglobin level increased to 9.3 g/dL. The patient's sample was referred to a reference laboratory and the antibody was identified as anti-JMH. He was successfully transfused with 6 additional units of least-incompatible RBCs without complication. HTLA antibodies against high frequency antigens, such as anti-JMH, are less likely to cause significant destruction of transfused antigen positive RBCs. However, identifying the specificity of these antibodies is necessary to appropriately understanding the clinical significance of the antibody, detecting other clinically important alloantibodies that may coexist, and determining the appropriate blood for transfusion.

Establishment of canine kidney cell line for canine distemper virus replication / 동물의과학연구지

Kidney cells of canine embryos were separated into single cells using collagenase and dispase. Primary culture was conducted using these cells. To remove fibroblasts, these cells were treated with edetate disodium dihydrate (Na2EDDA), and pure epithelial cells were separated. Recombinant retrovirus particles that manifest teromerase were produced and inoculated into primary culture cells to produce immortalized canine cell strains (JNUCK-1 and JNUCK-2). To examine the characteristics of the produced cell strains, the growth curve, maximum cultured households, and expressed proteins (keratin) were identified. The JNUCK-1 and JNUCK-2 cell lines showed division ability until the 30th generation without growth retardation. JNUCK-1 and JNUCK-2 cell lines clearly expressed telomerase until the 25th generation. The canine distemper virus (CDV) was inoculated into the JNUCK-1 and JNUCK-2 cell lines, as well as in the Madin-Darby canine kidney (MDCK) cell line. The maximum titer of CDV from the JNUCK-1 cell strain was about 200 times higher than that from the MDCK cell strain. However, the JNUCK-2 cell strain produced a lower titer than the MDCK cell strain. We established a new canine kidney epithelial cell line (JNUCK-1) that could produce CDV with high titer.