RESUMEN
The suppressive effects of different concentrations of aqueous leaf extracts of Neem (Melia azadirachta L.) on cow pea (Vigna unguiculata L.) and horse gram (Dolichos biflorus L.).The experiment was conducted in sterilized petridishes with a photoperiod of 24 hours on an average temperature. The result showed that aqueous leaf extract of Melia azadirachta caused siginifican inhibitory effect on seed germination, root and shoot elongation and fresh & dry weight of receptor plants. The effect was proportional to the concentrations of the extracts and higher concentration had the stronger inhibitory effect. The study also revealed that inhibitory effect was much pronounced in shoot development than the root of cow pea. Likewise root development greatly inhibited than the shoot in horse gram.
RESUMEN
An inhibitor of trypsin and chymotrypsin was purified from horse gram (Dolichos biflorus) beans. The concentration of the inhibitor which provided total inhibition was 0.27 μg/μg tryptic enzyme and 0.46 μg/μg chymotryptic enzyme. The inhibitor was stable at 37°C between pH of 3 to 11 and at 97°C, upto pH 5.0 only. While the activities were rapidly lost in 0.1Ν NaO H the loss was only 5 0% in 0.1Ν HCl when kept for 2 h at 97°C. On heating at pH 7.8, it remained stable upto 80°C with a gradual loss in activities at 97°C and a total loss occurring by autoclaving at 15 psi for 10 min. Reduction of disulphide bonds by 2-mercaptoethanol, pronase digestion and boiling in the presence of 1 Μ NaCl led to reduction in the activities. However, the inhibitor was resistant to the action of pepsin and subtilisin and to urea at 37°C.