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Objective To study the efficacy of interferon Alfa-lb( IFN?-lb) combined with lamivudine in the treatment of chronic hepatitis B. Methods 103 patients with chronic hepatitis B were divided into three groups which were comparable.30 patients were treated with IFN?-lb for about 6 months;38 patients were treated with lamivudine for about 12 months; And the last 36 patients were treated with IFN?-lb and lamivudine. The serum ALT,HBV-DNA,HBV-Marks were studied using electrochemiluminescence technique,fluorescent quantitation poly-merase chain reaction(PCR) and so on. Results The serum level of ALT,HBV-DNA and HbsAg in patients which were treated with IFN?-lb and lamivudjne showed significantly lower than the other groups(P
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Objective To compare the effects of interferon alfa-1b(IFN?-1b) and pegylated IFN?-1b(PEG IFN?-1b) on inhibiting growth of human liver cancer cells in vivo.Methods Human liver cancer cells(HepG-2,2?l06/0.2ml) cultured in log phase were subcutaneously injected into the right rear flank of male BALB/C nude mice to establish the tumor model.After HepG-2 injection,the tumor growth was followed up for 14 days.The mice with HepG-2 tumor were randomly divided into placebo(n=7),PEG IFN?-1b(n=8) and IFN?-1b(n=8) groups.Mice received injection of placebo 0.2ml/mouse each time(three times a week),PEG IFN?-1b 150?g/mouse per injection(once a week) and IFN?-1b 50?g/mouse each time(three times a week),respectively,according to their grouping,and this regime lasted for five weeks.In order to evaluate anti-tumor activity,animals' body weight was measured every week,and the measurements of tumor volume and animals' body weight were continuously done till the mice were sacrificed at the end of the fifth week.Results PEG IFN?-1b and IFN?-1b induced a significant decrease in tumor volume and the animals' weight,the suppression rates onto tumor growth were 56%(2.190g vs.4.979g) and 42%(2.678g vs.4.979g),respectively.However,no significant difference was found in animals' weight among the three groups.Conclusion HepG-2(2?l06/0.2ml) injection into nude mice may induce a typical tumor model.PEG IFN?-1b(150?g/mouse per time,once a week) could significantly inhibit the growth of HepG-2 in vivo,and so does the IFN?-1b(50?g/mouse each time,three times per week).