RESUMEN
Objective To investigate any anti-inflammatory and curative effect of hyperbaric oxygen (HBO) therapy on mice with acute lung injury (ALI) induced by lipopolysaccaride endotoxin. Methods Fortysix Sprague-Dawley rats, half male and half female, were randomly divided into a normal saline group with 10 rats,an LPS group with 12 rats, and an HBO group with 24 rats. LPS was injected via the tail vein at 5 mg/kg weight to establish the ALI model. The HBO group rats were treated with HBO. Then leucocyte counts, arterial blood gas analysis, interleukin-8 (IL-8) in the blood and lung wet/dry weight (W/D) were compared among the 3 groups at the 3rd and 6th hours. Pathological changes were examined under a light microscope. Results Compared with the LPS group, blood oxygen and W/D were higher in the HBO group, the white blood cell, neutrophil and IL-8 all were lower, and inflammatory cell infiltration was less in lung slides from the HBO group. Conclusions HBO therapy can improve oxygen deficiency and has an anti-inflammatory effect. As a result it might have protective effect against ALI.
RESUMEN
Various chemical and gas-liquid chromatographic analyses indicate that the lipid A backbone of the lipopolysaccharide (LPS) isolated from Bacteriodes fragilis NCTC 9343 is chemically constituted by a ?1, 6-interlinked D-gluco-samine disaccharide. It is phosphorylated at its 1-position by a glycosidic-linkage while the ester-bound phosphate present generally in other lipid A is depleted. The lipid A is lower fatty acylated in the amount of 5.2 fatty acids per lipid A molecule (of which 0.73 forms 3-acyloxyacyl groups). The LPS containing such a lipid A component has been shown endowing with a weaker endotoxicity.
RESUMEN
The lipid A component of Re-lipopolysaccharide from S.typhimurium SL 1102 was prepared by hydrolysis with a 0.1 mol/L acetate buffer.Chemical as well as advanced l3C-,and 31P-NMR investigations revealed a ?1' ,6-interlinked D-glucosamine diasaccharide, which is substituted by two phosphate(P)groups, one being esterlinked to the non-reducing residue,and another bound glycosidically to the C1 of the reducing residue, is as the central backbone.Its structure is shown as follows:-P-GlcN(?1', 6)GlcN-P-The amino(2 and 2')and hydroxy(3 and 3')groups of the backone are substituted by 3-OH-14: 0, and unhydroxy fatty acids appear to be the subtituents of the hydroxy group of 3-OH-14 :0, in the means of formation of 3-acyloxyacyl ester and amides.
RESUMEN
0), 3-hydroxy-15-methyl-hexadecanoic (3-OH-l5-Me-l6 : 0) and 13-methyl-tetradecanoic acid(13-Me-14 s 0).Approximately one mole of 2-keto-3-deoxy-D-mannooctonate was detected after treatment of LPS with 48% hydrofluoric acid, indicating it was present as a phosphorylated molecule.