RESUMEN
Objective:To establish a high performance liquid chromatography （HPLC） fingerprint of branches of <italic>Juglans mandshurica</italic> and to evaluate the quality of the samples from different producing areas and in different harvest periods. Method:Chromatographic separation was performed on an Agilent Poroshell 120 SB-C<sub>18</sub> column （2.1 mm×100 mm， 2.7 μm） for gradient elution with mobile phase of 0.2% formic acid solution （A）-0.2% formic acid acetonitrile solution （B） （0-5 min， 5%-10%B； 5-25 min， 10%-16%B； 25-40 min， 16%-22%B； 40-45 min， 22%-45%B； 45-50 min， 45%-65%B； 50-52 min， 65%-100%B； 52-55 min， 100%B） at a flow rate of 0.3 mL·min<sup>-1</sup>. The column temperature was 30 ℃ and the detection wavelength was 270 nm. The quality of branches of <italic>Juylans mandshurica</italic> was evaluated by similarity evaluation， cluster analysis， principal component analysis and partial least squares-discriminant analysis. The chemical constituents of the samples were identified by HPLC coupled with quadrupole time-of-flight mass spectrometry （HPLC-Q-TOF-MS/MS）. The mass spectrometry was conducted in negative ion mode with electrospray ionization（ESI）. Data were acquired over a range of <italic>m</italic>/<italic>z</italic> 100-1 700 for MS and <italic>m</italic>/<italic>z</italic> 50-1 700 for MS/MS. Result:A total of 19 common peaks were confirmed in 40 batches of samples， and the similarity ranged from 0.430 to 0.995， of which the similarity of samples collected in spring and winter seasons （April， May and December） was greater than 0.90， while the similarity of most samples collected in summer （July to September） was low. Multivariate statistical analysis showed that the samples were divided into two groups according to the harvest time， but there was no obvious classification rule for the samples from different producing areas. The contents of most constituents in the samples collected in spring and winter were higher than those collected in summer. The result illustrated that different harvest periods had great influence on the quality of branches of <italic>J</italic>.<italic> mandshurica</italic>. Compared with the samples collected in summer， the quality of samples collected in spring and winter was better. A total of 22 peaks were proved to be the main constituents that contributed to the difference between samples collected in different seasons. A total of 83 chemical components were identified by HPLC-Q-TOF-MS/MS， including 49 tannins， 7 organic acids， 14 naphthalene derivatives， 1 flavonoid， 6 anthracene derivatives， 2 lignans， 3 diarylheptanoids and 1 saccharide. Totally 13 common peaks were identified. Of the peaks that contributed to discriminate samples collected in different season， 19 peaks were identified and most of them were tannins. Conclusion:The established HPLC fingerprint can provide useful information for the quality evaluation of branches of <italic>J</italic>.<italic> mandshurica</italic>. Tannin is the main constituents in the samples. Harvest period has great influence on the quality of branches of <italic>J</italic>.<italic> mandshurica</italic>.
RESUMEN
Various species of Garcinia Linn.have been widely used as food and folk medicines,for their significant economic and pharmaceutical values.As the development of modern analysis technology,many phytochemistry methods were applied in the research of Garcinia Linn.,in which especially the liquid chromatography coupled with mass spectrometry (LC-MS),was increasingly used in chemical study for its rapid and accurate analysis.In this article,based on the published articles,the LC-MS methods used in the study of Garcinia Linn.,including the qualitative analysis,new compounds discovery and quantitative analysis were reviewed,and it would provide some information for the study of C arcinia Linn.in the future.
RESUMEN
Salvia miltiorrhiza (Danshen) is a kind of Chinese materia medica (CMM), and there has been a long history of Danshen for wide use in the treatment of numerous diseases, especially the unique therapeutic effect on cardiovascular disorders. Salvianolic acids and tanshinones are the main effective components in Danshen, which attracts the researchers' more attention for its significant biological activity. Liquid chromatography coupled with mass spectrometry technique (LC-MS) is a rapid, sensitive, and comprehensive analytical method for the simultaneous separation and analysis, which has been widely used in the study on CMM component analysis. A large number of literatures of the application of LC-MS in the component analysis of Danshen are systematically summarized in this paper, so as to provide some indication for scientific interpretation of the effective material basis, and improve the quality control standard and comprehensive utilization of Danshen and its preparation.