A DIGE PROTEOMIC ANALYSIS FOR LOW-INTENSITY EXERCISE-TRAINED RAT SKELETAL MUSCLE / 体力科学

<b>Background:</b> Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. <b>Purpose:</b> Therefore, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after low-intensity exercise training (LIT). <b>Methods:</b> For five days, rats performed LIT, which consisted of two 3-h swimming exercise with45-m rest between the exercise bouts. 2D-DIGE analysis was conducted on epitrochlearis muscles excised eighteen hours after the final training exercise. <b>Results:</b> Proteomic profiling revealed that, out of 681 detected and matched spots, 22 proteins exhibited changed expression by LIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. <b>Conclusion:</b> The proteomic 2D-DIGE analysis following LIT identified expressions of skeletal muscle proteins, includingATPsynα, UQCRC1, dihydrolipoamide dehydrogenase, dihydrolipoamide acetyltransferase, that were not previously reported to change their expressions after exercise-training.

Evaluation on the effect of low intensity exercise training in coronary artery disease. Including the evaluation on significance of exercise training after CABG surgery / 体力科学

A study was conducted to evaluate the effects of exercise training for cardiac rehabilitation. 69 patients with coronary artery disease were assigned to a low intensity exercise training program, which lasted for 3 months. The target heart rate for the exercise program was set at 50-60% of maximal heart rate. For comparison, the study patients were divided into 2 groups. 25 patients who had undergone CABG were assigned to group A and 42 patients who had not undergone CABG were assigned to group B. A symptom-limited treadmill exercise test was performed before and after the exercise training program in each patient. Oxygen uptake (VO<SUB>2</SUB>), SBP, HR and PRP were measured at rest, submaximal workload and workload at the end point during the treadmill exercise test.<BR>In addition, FAT, LVI and PCI were calculated. The parameters measured before and after exercise training were compared in order to assess the effects of the exercise training program. Our findings showed that exercise training was effective for improving exercise tolerance and submaximal exercise capacity in patients with coronary artery disease, particularly those who had undergone CABG.