Molecular characterization of Aeromonas hydrophila isolates from diseased fishes in district Kasur, Punjab, Pakistan

Abstract Pakistan is an agricultural country and fisheries play a very important role in the economic development of the country. Different diseases are prevalent in Pakistani fish but information related to the causative agents is not well-known. Keeping in view the significance of bacterial pathogens as the causative agents of multiple fish diseases, the present study was conducted for identification, characterization and analysis of virulence genes of Aeromonas spp. isolated from diseased fishes. A total of fifty fish samples having multiple clinical indications were collected from different fish farms of district Kasur, Punjab Pakistan. For isolation of Aeromonas spp. samples were enriched and inoculated on Aeromonas isolation medium. Isolates were identified and characterized by different biochemical tests, Analytical Profile Index (API) 20E kit and Polymerase Chain Reaction (PCR) assays. All isolates were screened for three putative virulence genes including aerolysin (aer), haemolysin (hyl) and heat labile cytotonic enterotoxin (alt). Seven isolates of Aeromonas (A.) hydrophila were retrieved and identified based on API 20E. These isolates were further confirmed as A. hydrophila on the basis of PCR assays. Three isolates were detected positive for the presence of virulence genes (alt and hyl). Whereas aerolysin (aer) gene was not present in any of A. hydrophila isolates. The present study confirmed A. hydrophila as the causative agent of epizootic ulcerative syndrome and motile Aeromonas septicemia in fish farms of district Kasur, Punjab Pakistan. Moreover, detection of two virulence genes (alt and hyl) in A. hydrophila isolates is a threat for fish consumers of study area.

Resumo O Paquistão é um país agrícola, onde a pesca desempenha um papel muito importante para o desenvolvimento econômico. Diferentes doenças são prevalentes em peixes do Paquistão, mas as informações relacionadas aos agentes causadores não são bem conhecidas. Tendo em vista a importância dos patógenos bacterianos como agentes causadores de múltiplas doenças em peixes, o presente estudo foi conduzido para identificação, caracterização e análise de genes de virulência de isolados de Aeromonas spp. de peixes doentes. Foram coletadas 50 amostras de peixes com múltiplas indicações clínicas em diferentes fazendas do distrito de Kasur, Punjab, Paquistão. Para isolar Aeromonas spp., as amostras foram enriquecidas e inoculadas em meio de isolamento. Os isolados foram identificados e caracterizados por diferentes testes bioquímicos, kit Analytical Profile Index (API) 20E, e ensaios de reação em cadeia da polimerase (PCR). Todos os isolados foram selecionados para três genes de virulência putativos, incluindo aerolisina (aer), hemolisina (hyl) e enterotoxina citotônica termolábil (alt). Sete isolados de Aeromonas hydrophila foram recuperados e identificados com base no API 20E. Esses isolados foram posteriormente confirmados como A. hydrophila de acordo com ensaios de PCR. Três isolados indicaram a presença de genes de virulência (alt e hyl), enquanto o gene aerolisina (aer) não esteve presente em nenhum dos isolados de A. hydrophila. O presente estudo confirmou A. hydrophila como o agente causador da síndrome ulcerativa epizoótica e septicemia móvel por Aeromonas em fazendas de peixes, no distrito de Kasur, Punjab, Paquistão. Além disso, a detecção de dois genes de virulência (alt e hyl) em isolados de A. hydrophila é uma ameaça para os consumidores de peixes da área de estudo.

Molecular characterization of Aeromonas hydrophila isolates from diseased fishes in district Kasur, Punjab, Pakistan / Caracterização molecular de isolados de Aeromonas hydrophila de peixes doentes no distrito de Kasur, Punjab, Paquistão

Abstract Pakistan is an agricultural country and fisheries play a very important role in the economic development of the country. Different diseases are prevalent in Pakistani fish but information related to the causative agents is not well-known. Keeping in view the significance of bacterial pathogens as the causative agents of multiple fish diseases, the present study was conducted for identification, characterization and analysis of virulence genes of Aeromonas spp. isolated from diseased fishes. A total of fifty fish samples having multiple clinical indications were collected from different fish farms of district Kasur, Punjab Pakistan. For isolation of Aeromonas spp. samples were enriched and inoculated on Aeromonas isolation medium. Isolates were identified and characterized by different biochemical tests, Analytical Profile Index (API) 20E kit and Polymerase Chain Reaction (PCR) assays. All isolates were screened for three putative virulence genes including aerolysin (aer), haemolysin (hyl) and heat labile cytotonic enterotoxin (alt). Seven isolates of Aeromonas (A.) hydrophila were retrieved and identified based on API 20E. These isolates were further confirmed as A. hydrophila on the basis of PCR assays. Three isolates were detected positive for the presence of virulence genes (alt and hyl). Whereas aerolysin (aer) gene was not present in any of A. hydrophila isolates. The present study confirmed A. hydrophila as the causative agent of epizootic ulcerative syndrome and motile Aeromonas septicemia in fish farms of district Kasur, Punjab Pakistan. Moreover, detection of two virulence genes (alt and hyl) in A. hydrophila isolates is a threat for fish consumers of study area.

Resumo O Paquistão é um país agrícola, onde a pesca desempenha um papel muito importante para o desenvolvimento econômico. Diferentes doenças são prevalentes em peixes do Paquistão, mas as informações relacionadas aos agentes causadores não são bem conhecidas. Tendo em vista a importância dos patógenos bacterianos como agentes causadores de múltiplas doenças em peixes, o presente estudo foi conduzido para identificação, caracterização e análise de genes de virulência de isolados de Aeromonas spp. de peixes doentes. Foram coletadas 50 amostras de peixes com múltiplas indicações clínicas em diferentes fazendas do distrito de Kasur, Punjab, Paquistão. Para isolar Aeromonas spp., as amostras foram enriquecidas e inoculadas em meio de isolamento. Os isolados foram identificados e caracterizados por diferentes testes bioquímicos, kit Analytical Profile Index (API) 20E, e ensaios de reação em cadeia da polimerase (PCR). Todos os isolados foram selecionados para três genes de virulência putativos, incluindo aerolisina (aer), hemolisina (hyl) e enterotoxina citotônica termolábil (alt). Sete isolados de Aeromonas hydrophila foram recuperados e identificados com base no API 20E. Esses isolados foram posteriormente confirmados como A. hydrophila de acordo com ensaios de PCR. Três isolados indicaram a presença de genes de virulência (alt e hyl), enquanto o gene aerolisina (aer) não esteve presente em nenhum dos isolados de A. hydrophila. O presente estudo confirmou A. hydrophila como o agente causador da síndrome ulcerativa epizoótica e septicemia móvel por Aeromonas em fazendas de peixes, no distrito de Kasur, Punjab, Paquistão. Além disso, a detecção de dois genes de virulência (alt e hyl) em isolados de A. hydrophila é uma ameaça para os consumidores de peixes da área de estudo.

Breaking bad news in ophthalmology: a literature review / Comunicação de más notícias em oftalmologia: uma revisão de literatura

ABSTRACT Medical specialties have recognized that breaking bad news assists clinical practice by mitigating the impact of difficult conversations. This scenario also encourages various studies on breaking bad news in ophthalmology since certain ocular diagnoses can be considered bad news. Thus, the objective is to review the scientific literature on breaking bad news in ophthalmology. The literature databases like MEDLINE/PUBMED, EMBASE, LILACS, SCOPUS, COCHRANE, and SCIELO, were screened for related research publications. Two independent reviewers read all the articles and short-listed the most relevant ones. Seven articles, in the formats of original article, review, editorial, oral communication, and correspondence, were reviewed. Conclusively it reveals that ophthalmologists are concerned with communicating bad news effectively but lack related studies. Nevertheless, there is a growing realization that training in breaking bad news can increase physicians' confidence during communication, thus, benefiting the therapeutic relationship with the patient and his family. Therefore, it would be valuable to include breaking bad news training in the curriculum of residencies.

RESUMO O reconhecimento sobre a comunicação de más notícias como mitigadora de conversas difíceis por outras especialidades médicas, incentiva o estudo desta temática na oftalmologia. Sendo assim, o objetivo deste estudo é revisar a produção de pesquisas científicas sobre a comunicação de más notícias em oftalmologia. Para isso, foi realizada uma revisão de literatura. As bases de dados utilizadas foram MEDLINE/PUBMED, EMBASE, LILACS, SCOPUS, COCHRANE e SCIELO. Dois revisores independentes leram todos os artigos e selecionaram a amostra final. Sete artigos foram escolhidos nos formatos de artigo original, revisão, editorial, comunicação oral e correspondência. Os oftalmologistas estão preocupados em comunicar as más notícias de forma eficaz, mas faltam estudos sobre o tema. No entanto, há uma crescente percepção de que o treinamento de comunicação de más notícias aumenta a confiança dos médicos na comunicação, beneficiando a relação terapêutica. Portanto, seria valioso incluir este treinamento no currículo das residências.

Controversias Diagnósticas en la Clasificación Internacional de las Cefaleas, III Edición / Diagnostic controversies on the International Classifica tion of Headaches Disorders, III Edition

Resumen La revisión consideró los puntos de conflicto que pue dan llevar a confusión en el uso diario de la clasificación internacional de las cefaleas (ICHD-III). Se evaluaron tanto las controversias al momento de confeccionar los criterios como las superposiciones producidas tras su utilización en la práctica diaria, argumentado a través de bibliografía científica. Como puntos relevantes, la anamnesis de un paciente con cefalea debe indicar la intensidad del dolor como así también la duración del episodio doloroso y si su localización es estrictamente unilateral. Estos puntos podrán ser de ayuda en los casos de dolor moderado que no cumplan en forma absoluta los criterios para ninguna de las cefaleas primarias, dilema frecuente en la práctica diaria.

Abstract The review considered points of conflict that may lead to confusion in the daily use of the International Classification of Headache Disorders (ICHD-III). Both the controversies at the time of preparing the criteria and the overlaps produced after their use in daily practice were evaluated, argued through scientific bib liography. As relevant points, the anamnesis of a patient with headache should indicate the intensity of the pain as well as the duration of the painful episode and if its location is strictly unilateral. These points may be help ful in cases of moderate pain that do not fully meet the criteria for any of the primary headaches, a frequent dilemma in daily practice.

Caracterización del síndrome coronario agudo en pacientes octogenarios

Introducción: La edad representa uno de los mayores predictores de riesgo de complicaciones cardiovasculares en pacientes con síndrome coronario agudo. La prevalencia de este síndrome en el grupo de los pacientes octogenarios es elevada. Métodos: Estudio prospectivo de cohortes analítico de todos los pacientes ingresados en la unidad de cuidados coronarios intensivos del Hospital Universitario "Comandante Manuel Fajardo" de la Habana, entre el año 2016 y el 2020. Objetivo: Caracterizar la población de pacientes octogenarios con síndrome coronario agudo y las posibles asociaciones entre la ocurrencia de complicaciones intrahospitalarias no letales y los factores de riesgo cardiovasculares. Resultados: Prevaleció el sexo femenino (64,2 %), los antecedentes de hipertensión arterial (89,7 %), cardiopatía isquémica (66,7 %), y el tabaquismo (32 %). Se encontraron asociaciones estadísticas significativas entre el tipo de síndrome coronario agudo y la presencia de complicaciones cardiovasculares de cualquier tipo (p=0,006); el aumento de la creatinina se asoció con la presencia de complicaciones hemodinámicas (Mdn=97; Rango=97,52; p=0,012), así como también la fracción de eyección del ventrículo izquierdo mostró una asociación muy significativa con la presencia de complicaciones cardiovasculares de cualquier tipo (Mdn=59; Rango=63,3; p<0,001) y hemodinámicas (Rango=55,2; p<0,001). Conclusiones: Se caracterizó la población de pacientes octogenarios con síndrome coronario agudo con elevación del segmento ST se asoció con un aumento de las complicaciones cardiovasculares intrahospitalarias, de la misma manera que sucedió con el valor de la fracción de eyección del ventrículo izquierdo.

Introduction: Age represents one of the greatest predictors of risk of cardiovascular complications in patients with acute coronary syndrome. The prevalence of this syndrome in the group of octogenarian patients is high. Objective: To characterize the population of octogenarian patients with acute coronary syndrome and the possible associations between the occurrence of non-lethal intrahospital complications and cardiovascular risk factors. Methods: This is a prospective analytical cohort study of all patients admitted to the intensive coronary care unit of Manuel Fajardo University Hospital in Havana, from 2016 to 2020. Results: The female sex (64.2%), a history of arterial hypertension (89.7%), ischemic heart disease (66.7%), and smoking habits (32%) outweighed. Significant statistical associations were found between the type of acute coronary syndrome and the presence of cardiovascular complications of any type (p=0.006); the increase in creatinine was associated with the presence of hemodynamic complications (Mdn=97; Range=97.52; p=0.012), as well as the left ventricular ejection fraction showed a highly significant association with the presence of cardiovascular complications of any type (Mdn=59; Range=63.3; p<0.001) and hemodynamic (Range=55.2; p<0.001). Conclusions: The octogenarian population of patients with ST-segment elevation acute coronary syndrome was characterized and was associated with an increase in in-hospital cardiovascular complications, in the same way that it happened with the value of the left ventricular ejection fraction.

Cáncer gástrico en octogenarios ¿Es viable una cirugía con intención curativa?

El objetivo de la presente investigación es evaluar la asociación entre la edad octogenaria y la tasa de morbimortalidad posoperatoria y supervivencia a los 5 años en adultos mayores tratados mediante gastrectomía R0 D2 en el Instituto Nacional de Enfermedades Neoplásicas (INEN) durante el periodo 2000-2013. Se realizó un estudio observacional, retrospectivo, analítico de cohorte pareado, que incluye pacientes con diagnóstico de adenocarcinoma gástrico tratados mediante gastrectomía R0 D2 en el INEN durante los años 2000 a 2013. Un grupo compuesto por todos los pacientes octogenarios que cumplieron los criterios de inclusión (92) y otro grupo compuesto por pacientes no octogenarios, con edades entre 50 a 70 años por ser el pico de presentación para esta patología (276). En una proporción 1:3, pareados según sexo, estadio tumoral y tipo de gastrectomía, los cuales constituyen los principales factores que podrían influir en la sobrevida de esta población. Los octogenarios presentaron menor albúmina (p<0,002), menor hemoglobina preoperatoria (p<0,001) y mayor clase ASA (p<0,001). La tasa de mortalidad a 30 días fue mayor en los octogenarios, pero no estadísticamente significativa (4,1% vs 1,4%; p=0,099). La probabilidad acumulada de supervivencia a 5 años fue 56% para octogenarios y 58% para los controles (p=0,763). El estadio clínico ≥ III y complicación posoperatoria grado ≥3 por Clavien Dindo fueron factores asociados de supervivencia. En conclusión, los octogenarios presentan mayor tasa de morbilidad posoperatoria, principalmente de causa respiratoria. Las tasas de mortalidad posoperatoria y supervivencia global no difieren entre octogenarios y no octogenarios con cáncer de estómago tratados mediante gastrectomía R0 D2.

The objective was to evaluate the association between octogenarian age and the rate of postoperative morbidity and mortality and 5-year survival in older adults at the National Institute of Neoplastic Diseases (INEN) during the period 2000-2013. We developed an observational, retrospective, analytical, paired cohort study. It includes patients with gastric adenocarcinoma as diagnosis, treated by R0 D2 gastrectomy at INEN during the period 2000 to 2013. One group included all octogenarian patients who met the inclusion criteria (92) and the other group made up of non-octogenarian patients, aged between 50 to 70 years because it is the age peak for this pathology (276). In a 1:3 ratio, paired according to sex, tumor stage, and type of gastrectomy, which are the main factors that could influence survival in this population. Octogenarians had lower albumin level (p<0.002), lower preoperative hemoglobin (p<0.001) and higher ASA classification (p<0.001). 30 days mortality rate was higher in octogenarians but not statistically significant (4.1% vs 1.4%; p=0.099). The 5-year cumulative survival probability was 56% for octogenarians and 58% for non- octogenarians (p=0.763). Clinical stage ≥ III and postoperative complication grade ≥ 3 by Clavien Dindo scale were predictors of survival. In conclusion, octogenarians have a higher rate of postoperative morbidity, mainly for respiratory causes. Postoperative mortality and overall survival rates do not differ between octogenarians and non-octogenarians with stomach cancer treated by R0 D2 gastrectomy.

Supervisão acadêmica de médicos em áreas indígenas durante a pandemia de Covid-19 / Academic supervision of doctors in indigenous areas during the Covid-19 pandemic / Supervisión académica de médicos en áreas indígenas durante la pandemia de Covid-19

Trata-se de estudo qualitativo sobre o papel da supervisão acadêmica do Grupo Especial de Supervisão do Projeto Mais Médicos para o Brasil de Roraima (GES-RR) durante a pandemia de Covid-19. Foi realizada uma cartografia, com entrevistas e uma roda de conversa. Durante a pandemia, a Atenção à Saúde Indígena passou por rearranjos e a supervisão acadêmica do GES-RR exerceu-se remotamente, o que reduziu seu potencial, mas manteve sua relevância. O acolhimento dos médicos pelos supervisores potencializou a capacidade transformadora da assistência e diminuiu a sensação de isolamento e abandono. O GES-RR foi importante para a mediação de conflitos com a gestão, para a qualificação do trabalho médico, para a reflexão sobre as relações e condições de trabalho e como espaço privilegiado de Educação Permanente em Saúde. O estudo mostrou a importância dos papéis exercidos e da retomada presencial em momento oportuno.(AU)

We conducted a qualitative study to investigate the academic supervision role of the Special Supervision Group for the More Doctors Project for Brazil in Roraima (GES-RR) during the Covid-19 pandemic. We constructed a map based on interviews and conversation circles. During the pandemic, indigenous health care underwent reshaping and academic supervision was performed by the GES-PR remotely, reducing its potential but maintaining its relevance. The support provided to the doctors by the supervisors enhanced the transformative capacity of care and reduced the sensation of isolation and abandonment. The GES-RR played an important role in mediating conflicts with management, improving the quality of medical work, and stimulating reflection on working relations and conditions, and is uniquely positioned to provide permanent health education. The findings highlight the importance of the roles and of returning to face-to-face working at the appropriate time.(AU)

Se trata de un estudio cualitativo sobre el papel de la supervisión académica del Grupo Especial de Supervisión del Proyecto Más Médicos para Brasil de Roraima (GES-RR) durante la pandemia de Covid-19. Se realizó una cartografía, con entrevistas y una ronda de conversaciones. Durante la pandemia, la Atención de la Salud Indígena pasó por reorganizaciones y la supervisión académica del GES-RR se ejerció remotamente, pero mantuvo su relevancia. La acogida a los médicos por parte de los supervisores potencializó la capacidad transformadora de la asistencia y disminuyó la sensación de aislamiento y abandono. El GES-RR fue importante en la mediación de conflictos con la gestión, calificación del trabajo médico, para la reflexión sobre las relaciones y condiciones de trabajo y como espacio privilegiado de Educación Permanente de Salud. El estudio mostró la importancia de los papeles ejercidos y de la retomada presencial en momento oportuno.(AU)

Effects of Tingli dazao xiefei decoction on ventricular remodeling in model rats with heart failure after myocardial infarction through ACE2-Ang（-1-7）-Mas axis / 中国药房

OBJECTIVE To explore the mechanism of Tingli dazao xiefei decoction on ventricular remodeling in model rats with heart failure after myocardial infarction. METHODS The rat model of heart failure after myocardial infarction was established by ligation of anterior descending branch of left coronary artery， which was divided into 8 groups： sham operation group， model group， A779 group （1 mg/kg）， A779 （1 mg/kg）+Tingli dazao xiefei decoction equivalent-dose group （0.8 g/kg）， A779 （1 mg/kg） +Tingli dazao xiefei decoction high-dose group （1.6 g/kg）， Tingli dazao xiefei decoction equivalent-dose group （0.8 g/kg）， Tingli dazao xiefei decoction high-dose group （1.6 g/kg） and losartan potassium group （10 mg/kg）. Each group was given equal volume of distilled water or corresponding drugs intragastrically for 4 weeks. Masson staining was used to determine the distribution of collagen fibers in rat myocardium. The content of hydroxyproline （Hyp） in myocardium was determined by alkaline hydrolyzation. The expressions of type Ⅰ and Ⅲ collagen （COLⅠ， COLⅢ）in myocardium were detected by immunohisto-chemistry. Myocardial fibrosis-related indexes such as matrix metalloproteinase-2 （MMP-2）， MMP-9， tissue inhibitor of metalloproteinase-1 （TIMP-1） and soluble suppression of tumorigenicity-2 （sST-2） were detected by ELISA. The protein expressions of angiotensin converting enzyme 2-angiotensin-（1-7）-Mas [ACE2-Ang-（1-7）-Mas] axis were detected by Western blot. RESULTS Compared with sham operation group， myocardial cells in model group and A779 group were disordered， collagen fiber deposition was significantly increased and myocardial fibrosis was obvious； the Hyp content and MMP-2， MMP-9， sST-2 levels were increased， and COL Ⅰ and COL Ⅲ positive expressions were significantly enhanced； TIMP-1 level， protein expressions of ACE2， Ang-（1-7） and Mas were significantly decreased （P＜0.05）. Compared with model group， above indexes of Tingli dazao xiefei decoction equivalent-dose and high-dose groups were improved to different extents. Compared with A779 group， A779+Tingli dazao xiefei decoction equivalent-dose and A779+high-dose groups could improve myocardial arrangement and collagen distribution， reduce the Hyp content and MMP-2， MMP-9 levels， reduce positive expressions of COL Ⅰ and COL Ⅲ （P＜0.05）， but couldn’t improve Ang-（1-7） and Mas protein expression. CONCLUSIONS Tingli dazao xiefei decoction can improve ventricular remodeling in myocardial failure model rats after myocardial infarction by improving the expression of ACE2- Ang（- 1-7）-Mas axis proteins.

Buzhong Yiqitang Reduces CIH-induced Pulmonary Inflammatory Injury by ACE2-Ang（1-7）-Mas Axis / 中国实验方剂学杂志

ObjectiveTo investigate the intervention effect of Buzhong Yiqitang （BZYQT） on pulmonary inflammation in mice induced by chronic intermittent hypoxia （CIH） and preliminarily elucidate its mechanism. MethodForty healthy male C57BL/6 mice aged 6-8 weeks were randomly divided into the following groups： normoxia group， model group （exposed to CIH）， and low-， medium-， and high-dose BZYQT groups. The normoxia group was exposed to a normoxic environment， while the model group and the low-， medium-， and high-dose BZYQT groups were exposed to intermittent hypoxia. In the BZYQT groups， the BZYQT （8.1， 16.2， 32.4 g·kg-1·d-1） was administered orally 30 min before placing the mice in the hypoxic chamber， while the model group and the normoxia group received an equivalent volume of normal saline. After five weeks of modeling， pulmonary function of the mice was measured using an EMKA animal lung function analyzer， and lung tissue samples were collected after the pulmonary function tests. Hematoxylin-eosin （HE） staining was performed to observe the histopathological changes in the lung tissue of each group. Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of interleukin-6 （IL-6）， interleukin-8 （IL-8）， tumor necrosis factor-α （TNF-α） in the serum， as well as angiotensin Ⅱ （Ang Ⅱ） and angiotensin-（1-7） ［Ang（1-7）］ in lung tissue. Western blot and immunohistochemistry were used to detect the protein expression of IL-6， IL-8， TNF-α， angiotensin-converting enzyme 2 （ACE2）， and mitochondrial assembly receptor （Mas）. ResultCompared with the normoxia group， the model group showed significant abnormalities in lung function （P<0.05， P<0.01）， lung tissue changes， such as thickening of alveolar walls and inflammatory cell infiltration， increased levels of IL-6， IL-8， TNF-α in the serum and Ang Ⅱ in lung tissue （P<0.01）， decreased level of Ang（1-7） （P<0.01）， increased protein expression of IL-6， IL-8， and TNF-α， and decreased protein expression of ACE2 and Mas （P<0.05， P<0.01）. Compared with the model group， the BZYQT groups showed improvement in lung function （P<0.05， P<0.01）， and HE staining of lung tissue showed approximately normal alveolar wall thickness and reduced inflammatory cell infiltration. Immunohistochemistry and Western blot analysis showed a significant decrease in the expression of inflammatory-related proteins （P<0.05， P<0.01）， and a significant increase in ACE2 and Mas protein expression （P<0.05， P<0.01）. ConclusionBZYQT can improve lung injury in mice exposed to CIH by regulating the ACE2-Ang（1-7）-Mas axis to inhibit inflammatory responses.

Effects and mechanism of anaphylactoid reaction induced by nonapeptide activating mast cells / 中国药房

OBJECTIVE To study the effects and potential mechanism of anaphylactoid reaction induced by nonapeptide IVQKIKHCF activating mast cells. METHODS Using human mast cell line LAD2 as subject， and substance P as positive control， the activation effects of 25， 50 and 100 μmol/L IVQKIKHCF on mast cells were investigated by determining the release rate of β-aminohexosidase， histamine release， and the contents of inflammatory factors； using MrgprX2-knockdown LAD2 cells and Mas- related G protein-coupled receptor X2 （MRGPRX2） high-expression human embryonic kidney cell line HEK293 （MRGPRX2/ HEK293 cells） as subject， the correlation between the activation effect of IVQKIKHCF and MRGPRX2 was investigated by determining the release rate of β-aminohexosidase， and intracellular calcium ion concentration. RESULTS IVQKIKHCF with 25， 50， 100 μmol/L could significantly increase the release rate of β-aminohexosidase and histamine release in LAD2 cells （P＜0.05）， and promote the release of tumor necrosis factor-α， interleukin-8， macrophage inflammatory protein-1β and monocyte chemotactic protein-1 to varying degrees （P＜0.05）. After knocking down MrgprX2， the effects of 25， 50， 100 μmol/L IVQKIKHCF promoting the release of β-aminohexosidase in LAD2 cells were reversed significantly （P＜0.05）， resulting in an increase of calcium ion concentration in MRGPRX2/HEK293 cells. CONCLUSIONS Nonapeptide IVQKIKHCF can promote mast cells to release granular matter and inflammatory mediators by activating MRGPRX2 thus inducing anaphylactoid reaction.

Prevalence of non-communicable chronic diseases: arterial hypertension, diabetes mellitus, and associated risk factors in long-lived elderly people / Prevalencia de enfermedades crónicas no transmisibles: hipertensión arterial, diabetes mellitus y factores de riesgo asociados en ancianos longevos / Prevalência das doenças crônicas não transmissíveis: hipertensão arterial, diabetes mellitus e fatores de risco associados em pessoas idosas longevas

ABSTRACT Objective: To identify the prevalence of non-communicable chronic diseases: arterial hypertension, diabetes mellitus, and associated risk factors in long-lived elderly people from three Brazilian regions. Methods: This is a multicenter, cross-sectional, and comparative study conducted with elderly people aged 80 years or older. Results: Higher prevalence of arterial hypertension were observed among those who use polypharmacy (75.7%), among elderly people aged between 80 and 84 years (33.9%), as well as in elderly people who are overweight (78.2%). The prevalence of diabetes was 24% (RP: 0.76; 95% CI: 0.59-0.98) lower among women compared to men and 2.15 times higher among those who use five or more medications (RP: 2.15; 95% CI: 1.63-2.85). Conclusions: In our sample, polypharmacy, body weight, and gender determine the prevalence of non-communicable chronic diseases: arterial hypertension and diabetes mellitus in long-lived elderly people.

RESUMEN Objetivo: Identificar la prevalencia de enfermedades crónicas no transmisibles: hipertensión arterial, diabetes mellitus y los factores de riesgo asociados en ancianos longevos de tres regiones brasileñas. Métodos: Se trata de un estudio multicéntrico, transversal y comparativo, realizado con ancianos con edad igual o superior a 80 años. Resultados: Se observaron mayores prevalencias de hipertensión arterial entre aquellos que hacen uso de polifarmacia (75,7%), entre los ancianos con edad entre 80 y 84 años (33,9%), así como en ancianos que presentan sobrepeso (78,2%). La prevalencia de diabetes fue 24% (RP: 0,76; IC 95%: 0,59-0,98) menor entre las mujeres en comparación con los hombres y 2,15 veces mayor entre aquellos que utilizan cinco o más medicamentos (RP: 2,15; IC 95%: 1,63-2,85). Conclusión: En nuestra muestra, la polifarmacia, el peso corporal y el sexo determinan la prevalencia de las enfermedades crónicas no transmisibles: hipertensión arterial y diabetes mellitus en ancianos longevos.

RESUMO Objetivo: identificar a prevalência das doenças crônicas não transmissíveis: hipertensão arterial, diabetes mellitus e os fatores de risco associados em pessoas idosas longevas de três regiões brasileiras. Métodos: trata-se de um estudo multicêntrico, transversal e comparativo, realizado com pessoas idosas com idade igual ou superior a 80 anos. Resultados: foram observadas maiores prevalências de hipertensão arterial entre aqueles que fazem uso de polifarmácia (75,7%), entre as pessoas idosas com idade entre 80 e 84 anos (33,9%), bem como em pessoas idosas que apresentam sobrepeso (78,2%). A prevalência de diabetes foi 24% (RP: 0,76; IC 95%: 0,59-0,98) menor entre as mulheres quando comparadas aos homens e 2,15 vezes maior entre aqueles que utilizam cinco ou mais medicamentos (RP: 2,15; IC 95%: 1,63-2,85). Conclusões: Em nossa amostra, a polifarmácia, o peso corporal e o sexo determinam a prevalência das doenças crônicas não transmissíveis: hipertensão arterial e diabetes mellitus em pessoas idosas longevas.

Uso ambulatorio de medicamentos potencialmente inapropiados en adultos mayores usuarios de la RAP Metropolitana de ASSE durante 2019 / Potentially inappropriate medication use in older adults assisted as outpatients in ASSE's Metropolitan RAP (Primary Health Care) during 2019 / Uso ambulatorial de medicamentos potencialmente inapropriados em idosos usuários da RAPE Metropolitana da ASSE durante 2019

Introducción: los adultos mayores son una población de riesgo para el desarrollo de reacciones adversas a los medicamentos. Los medicamentos potencialmente inapropiados son aquellos que representan mayores riesgos que beneficios en este grupo etario. Se cuenta con herramientas de apoyo a la prescripción en geriatría que permiten identificar a estos medicamentos y mediante la aplicación de estudios de utilización de medicamentos, podemos describir o analizar el uso de los mismos en una población. Objetivos: reconocer disponibilidad de medicamentos potencialmente inapropiados para adultos mayores en la RAP metropolitana de ASSE durante 2019 y establecer un diagnóstico de situación de consumo de los mismos durante ese año. Método: se realizó un análisis del vademécum institucional mediante la aplicación de los Criterios de Beers 2019 y dos escalas de riesgo anticolinérgico para identificar medicamentos potencialmente inapropiados. Posteriormente se realizó un estudio de utilización de los medicamentos identificados, mediante datos de dispensación de farmacia entre el 1 de enero y 31 de diciembre de 2019. El consumo se expresó en Dosis Diarias Definidas por cada 1000 adultos mayores-año (DHD). Resultados: se identificaron 16 medicamentos potencialmente inapropiados, de los cuales los más usados fueron clonazepam (DHD 69), quetiapina (65,6), alprazolam (DHD 43,7), flunitrazepam (DHD 42,7) y zolpidem (DHD 36,4). Conclusiones: la aplicación de herramientas explícitas facilita la identificación de medicamentos potencialmente inapropiados para adultos mayores y se evidenció un consumo elevado de los mismos durante el año 2019 a expensas de derivados benzodiazepínicos y quetiapina.

Introduction: older adults are at higher risk for developing adverse drug reactions. Potentially inappropriate medications are drugs that have more risks than benefits in this age group. There are a number of tools to support the prescription of medication in geriatrics that allow the identification of these medications, and by applying studies developed on the use of medications we may describe or analyze their impact on a given population. Objectives: to recognize availability of potentially inappropriate medications in older adults in ASSE's Metropolitan RAP during 2019 and to draw conclusions about the current situation in terms of the consumption of this kind of medications. Method: an institutional analysis of medications available in each healthcare provided was conducted through the application of Beers Criteria 2019, and two anticholinergic risk scales were used to identify potentially inappropriate medications. Subsequently, the use of the medications identified was studied by applying pharmacy dispensing data between January 1 and December 31, 2019. Consumption was expressed in defined daily doses every 1000 adults per year (DHD). Results: 16 potentially inappropriate medications were identified, the most widely used of which were clonazepam (DHD 69), quetiapine (65.6), alprazolam (DHD 43.7), flunitrazepam (DHD 42.7) and zolpidem (DHD 36.4). Conclusions: Applying explicit tools makes it easier to identify potentially inappropriate medications for older adults. An increased consumption of these kinds of drugs was noticed during 2019, as a result of benzodiazepine derivatives and quetiapine.

Introdução: os idosos são uma população de risco para o desenvolvimento de reações adversas a medicamentos. Medicamentos potencialmente inapropriados são aqueles que apresentam maiores riscos do que benefícios nessa faixa etária. Existem ferramentas de apoio à prescrição em geriatria que permitem identificar esses medicamentos e, por meio da aplicação de estudos de utilização de medicamentos, descrever ou analisar seu uso em uma população. Objetivos: reconhecer a disponibilidade de medicamentos potencialmente inapropriados para idosos na RAP metropolitana da ASSE durante o ano de 2019 e estabelecer um diagnóstico de consumo durante esse ano. Método: o formulário institucional foi analisado utilizando os Critérios de Beers 2019 e duas escalas de risco anticolinérgico para identificar medicamentos potencialmente inapropriados. Posteriormente, foi realizado um estudo de consumo dos medicamentos identificados, através dos dados de dispensação da farmácia entre 1 de janeiro e 31 de dezembro de 2019. O consumo foi expresso em Doses Diárias Definidas por 1000 idosos-ano (DHD). Resultados: foram identificados 16 medicamentos potencialmente inapropriados, sendo clonazepam (DHD 69), quetiapina (65,6), alprazolam (DHD 43,7), flunitrazepam (DHD 42,7) e zolpidem (DHD 36,4) os mais utilizados Conclusões: a aplicação de ferramentas explícitas facilita a identificação de medicamentos potencialmente inapropriados para idosos; foi observado um alto consumo dos mesmos em detrimento dos derivados benzodiazepínicos e da quetiapina durante o período do estudo.

Ang-(1-7) attenuates podocyte injury induced by high glucose in vitro

ABSTRACT Objective: The incidence of diabetic nephropathy (DN) is gradually increasing worldwide. Podocyte injury, such as podocyte apoptosis and loss of the slit diaphragm (SD)-specific markers are early pathogenic features of DN. Materials and methods: The cultured mouse podocytes were separated into a high glucose-treated (HG, 30mM) group to mimic DN in vitro, a low glucose-treated (LG, 5mM) group as a control and HG+ angiotensin-(1-7)(Ang-(1-7)) and HG+Ang-(1-7) + D-Ala7-Ang-(1-7) (A779, Ang-(1-7)/Mas receptor antagonist) experimental groups. The Cell Counting Kit-8 (CCK-8) method and flow cytometry was used to detect podocyte activity and podocyte apoptosis respectively. The expression of angiotensin type 1 receptor (AT1R), Mas receptor (MasR) and podocyte-specific markers were examined by q-PCR and Western blot, respectively. Results: The results showed that the decrease in podocyte activity; the increase in podocyte apoptosis; the decreased mRNA and protein expression of nephrin, podocin, WT-1 and MasR; and the upregulated expression of AT1R induced by HG could be reversed by Ang-(1-7). However, these effects were blocked by A779. The possible mechanisms of the Ang-(1-7)-mediated effect depended on MasR. In addition, the protective effect of Ang-(1-7) on podocyte activity was dose-dependent and most obvious at 10 µM. A779 had the greatest antagonistic action against Ang-(1-7) at a concentration of 10 μM. Conclusion: This study reveals that binding of Ang-(1-7) to its specific receptor MasR may counteract the effects of Ang II mediated by AT1R to significantly attenuate podocyte injury induced by high glucose. Ang-(1-7)/MasR targeting in podocytes may be a therapeutic approach to attenuate renal injury in DN.

Opciones de Producción Más Limpia en la Universidad de Ciencias Médicas de Matanzas

Introducción: la sostenibilidad ambiental es una de las metas del milenio establecidas por la Asamblea General de las Naciones Unidas. La minimización de residuos, la producción más limpia, la ecoeficiencia y la prevención de la contaminación contribuyen a su cumplimiento. En el sector de la salud, la Producción Más Limpia se enfoca en la prevención de la contaminación, y en la eliminación de desechos peligrosos, infecciosos y químicos y de emisiones o vertimientos dentro de la institución; se fundamenta en buenas prácticas durante la prestación de servicios, en la sustitución de insumos con características peligrosas, en cambios en los procedimientos y en mejoramiento tecnológico. Objetivo: proponer opciones de Producción Más Limpia en la Universidad de Ciencias Médicas de Matanzas. Materiales y métodos: se desarrolló una investigación de tipo cualitativa, descriptiva y documental. La búsqueda se realizó en la base de datos SciELO y en Google Académico, en el período 2016-2020. Resultados: se identificaron cinco opciones de Producción Más Limpia con resultados satisfactorios que contribuyen a la sostenibilidad ambiental de la Universidad. Conclusiones: la introducción de nuevas tecnologías, la sustitución de sustancias contaminantes, el desarrollo energético y la separación correcta de los residuos, son opciones de Producción Más Limpia que favorecen el desarrollo sostenible.

Introduction: environmental sustainability is one of the millennium goals set by the United Nations General Assembly. Waste minimization, cleaner production, eco-efficiency and pollution prevention contribute to its compliance. In the health sector, Cleaner Production focuses on the prevention of pollution, the disposal of hazardous, infectious and chemical wastes and emissions or discharges within the institution; it is based on good practices during the provision of services, on the replacement of inputs with hazardous characteristics, on changes in procedures and on technological improvement. Objective: to propose Cleaner Production options at Matanzas University of Medical Sciences. Materials and methods: a qualitative, descriptive and documentary research was developed. The search was carried out in the SciELO database and in Google Scholar, in the period 2016-2020. Results: five Cleaner Production options were identified with satisfactory results that contribute to the environmental sustainability of the University. Conclusions: the introduction of new technologies, the replacement of pollutants, energy development and the correct separation of waste are Cleaner Production options that favor sustainable development.

La importancia de la comunicación médico-paciente: un estudio en Argentina

Objetivo: Analizar la importancia de la comunicación en la relación médico-paciente y recopilar información sobre la percepción durante su práctica. Método: Se realiza una revisión bibliográfica y una encuesta (participaron 105 médicos en Argentina). Resultados: Los resultados muestran que la mayoría de los profesionales refiere dificultades en la comunicación y que estas dificultan la atención. Los médicos manifestaron dificultades para comunicar malas noticias, mayormente en las áreas clínicas, y que la habilidad de ponerse en el lugar del paciente es fundamental para la comunicación de dichas noticias. Los jóvenes consideran de manera significativa que la comunicación de malas noticias depende de la práctica. Conclusiones: Las habilidades comunicativas son indispensables. Existe evidencia para sugerir que es necesario profundizar en la adquisición de competencias comunicativas y valores éticos.

Objective: To discuss the importance of communication in the doctor-patient relationship and collect information about perception during their practice. Materials and methods: A literature review and a survey were conducted (105 doctors in Argentina participated). Results: Most professionals report communication difficulties, which hinder care. Physicians expressed difficulties communicating bad news, primarily in clinical areas, and the ability to put themselves in the patient's shoes is essential for giving such news. Young people significantly consider that the communication of bad news depends on practice. Conclusions: Communication skills are critical. Evidence suggests that it is necessary to look into the acquisition of communication skills and ethical values.

Objetivo: analisar a importância da comunicação na relação entre médico e paciente, e coletar informações sobre a percepção durante sua prática. Método: são realizados uma revisão bibliográfica e um questionário, do qual participaram 105 médicos na Argentina. Resultados: a maioria dos profissionais refere dificuldades na comunicação e que estas prejudicam o atendimento. Os médicos manifestaram dificuldades para comunicar más notícias, predominantemente nas áreas clíngicas, e que a habilidade de se colocar no lugar do paciente é fundamental para a comunicação dessas notícias. Os jovens consideram de maneira significativa que a comunicação de más notícias depende da prática. Conclusões: as habilidades comunicativas são indispensáveis. Há evidência para sugerir que seja necessário aprofundar sobre a aquisição de competências comunicacionais e valores éticos.

Revisión de los factores de riesgo y factores protectores para el cáncer de mama

Objetivo: Revisar los aspectos clínicos necesarios a tomar en cuenta por el médico tratante al interpretar los reportes de los hallazgos radiológicos en la mamografía y de otras pruebas diagnósticas para un diagnóstico integral del cáncer de mama. Métodos: Se realizó una revisión sistemática de manuscritos publicados entre el 2010 y el 2020 sobre factores de riesgo y factores protectores que pueden verse involucrados en la incidencia del cáncer de mama. Se revisaron publicaciones de investigaciones realizadas en mujeres con diagnóstico de cáncer de mama de tipo ensayos clínicos aleatorizados, casos y controles, revisiones sistemáticas, metaanálisis y guías internacionales. Se buscó en las bases de datos bibliográficas PubMed, EBSCO y Scopus, publicaciones en inglés o español, utilizando términos provenientes de los Descriptores en Ciencias de la Salud (DeCS). Resultados: Se identificaron un total de 735 artículos en las búsquedas, de los cuáles se excluyeron 508 por su poca relevancia de acuerdo con el título. De los 227 restantes se excluyeron 22 por estar repetidos en las distintas bases, 12 porque no cumplían con los criterios de inclusión, 83 durante la revisión del resumen, y 28 al revisar el texto completo. En total se incluyeron 82 artículos para elaborar esta revisión. Los factores identificados en la revisión de la literatura se clasificaron en tres áreas: fisiopatológicos, gíneco-obstétricos y ambientales. Los factores de riesgo fisiopatológicos son la historia familiar, hiperplasia epitelial atípica, etnia, mayor edad, diabetes tipo II, síndrome metabólico, mayor peso y talla y mutaciones genéticas; los gíneco-obstétricos son la menarquía temprana, la menopausia tardía y la nuliparidad; y los ambientales incluyen fármacos, hábitos de vida, procedimientos médicos y alimentación. Mientras que, entre los factores protectores, los factores fisiopatológicos son el hipotiroidismo, el asma y la rinitis alérgica; los gínecoobstétricos son la multiparidad, el primer embarazo a una edad temprana y dar lactancia; y los ambientales incluyen fármacos, procedimientos médicos y alimentación.

Aim: To review relevant clinical aspects for the attending physician to consider when interpreting the reports of radiological findings in the mammogram and other diagnostic tests for a comprehensive breast cancer diagnosis. 2 ISSN 0001-6012 • eISSN 2215-5856 / Acta méd. costarric. 2022 / octubre-diciembre; 64 (4): 1-11 Cáncer mama factores de riesgo y de protección Methods: A systematic literature review was carried out focusing on risk and protective factors associated with breast cancer incidence, within peer-reviewed scientific articles published between 2010 and 2020. Published research on women with a breast cancer diagnoses who participated in randomized clinical trials, case control studies, systematic reviews, meta-analysis, and international guides were used. Research terms were searched on PubMed, EBSCO and Scopus databases, published in Spanish and English, using terms from the Medical Subject Headings (MeSH). Results: A total of 735 publications were found of which 508 were excluded due to title content being irrelevant to the review. Of the remainder 227, 22 were excluded due to been repeated across the different databases used, 12 because they did not meet the inclusion criteria, 83 after abstract review, and 28 after reading the whole text. A total of 82 papers were used for this review. The identified factors were categorized in three areas: pathophysiological, gynecological and obstetrics, and environmental factors. The pathophysiological risk factors are, family history, atypical hyperplasia, ethnicity, age, diabetes type II, metabolic syndrome, greater weight and height and genetic mutations; the gynecological and obstetric factors are early menarche, late menopause and nulliparity; and the environmental factors include prescription drugs, lifestyle habits, medical procedures and eating habits. Regarding the protector factors, the pathophysiological factors include hypothyroidism, asthma and allergic rhinitis; in the gynecological and obstetrics category are multiparity, the first pregnancy at early age and breast feeding; lastly, the environmental factors include prescription drugs, medical procedures and eating habits. Conclusions: Considering the risk and protector factors for breast cancer would allow a more comprehensive approach in the diagnosis and treatment of breast cancer.

Responsabilização em más condutas científicas: opinião de editores do SciELO / Accountability to scientific misconduct: the SciELO editors' view / Responsabilidad por malas conductas científicas: opinión de los editores de SciELO

Responsabilização (accountability) em comunicação e editoração científica é um importante tema em ética e integridade em pesquisa, e um dos grandes desafios atuais da ciência. Este artigo apresenta um estudo descritivo sobre a responsabilização e os seus atores na comunicação científica (autores, revisores, editores), partindo da questão da responsabilização desses atores e das más condutas científicas vista por editores de revista SciELO do Brasil e de países da América Latina. Apresenta resultados a partir da visão dos editores em que os autores são responsáveis, e, parcialmente, os revisores e os próprios editores

Accountability in scientific communication and publishing is an important topic in ethics and research integrity, and one of the great challenges of science today. This article presents a descriptive study on accountability and its actors in scientific communication (authors, reviewers, editors), starting from the issue of accountability of these actors and from the scientific misconducts viewed by SciELO journals editors in Brazil and Latin America. Based on the editors' view, it stands that the authors are responsible, and the reviewers and the editors are partially responsible

La responsabilidad (accountability) en la comunicación y publicación científica es un tema importante en la ética y la integridad en la investigación, y uno de los grandes desafíos de la ciencia actual. Este artículo presenta un estudio descriptivo sobre la responsabilidad y sus actores en la comunicación científica (autores, revisores, editores), a partir de la cuestión de responsabilidad de estos actores y de las malas conductas científicas vista por los editores de revista SciELO en Brasil y en países de América Latina. Presenta resultados en la visión de los editores de que los autores son responsables, y los revisores y los propios editores son parcialmente responsables.

Recomendaciones en la primera consulta en personas adultas con obesidad / Recomendations for the first appointment of adult persons with obesity

El objetivo de esta recomendación es establecer las mejores estrategias para el abordaje de la obesidad en la primera consulta de un paciente adulto con obesidad. Para ello se formularon tres preguntas PICO con respuestas basadas en el análisis de la evidencia científica disponible. Nuestros principales hallazgos fueron: • En la primera consulta de un paciente adulto con obesidad, la entrevista motivacional es más efectiva frente al abordaje tradicional para el descenso de peso dado que, además, permite reforzar la motivación del paciente y estimular su participación en un cambio de comportamiento. • Para el diagnóstico de obesidad, el índice de masa corporal (IMC) sigue siendo una herramienta útil y sencilla de detección, sin embargo, es imperativo ampliar la visión de la obesidad y establecer el riesgo de complicaciones en la primera consulta; para esto tanto el sistema de estadificación de Edmonton como el método ABCD son herramientas útiles adicionales al IMC. • La actividad física aeróbica sigue siendo recomendada por su beneficio en la pérdida de masa grasa, principalmente visceral, no obstante, al combinar una actividad física anaeróbica, los resultados son superiores a la estrategia aeróbica aislada. Conclusiones: el abordaje de la obesidad en la primera consulta debería basarse en una entrevista motivacional para mejorar la adherencia; el diagnóstico de obesidad debería realizarse mediante el cálculo del IMC y una herramienta que permita predecir el riesgo de complicaciones; la actividad física debería combinar estrategias aeróbicas y anaeróbicas para mejorar los parámetros antropométricos y metabólicos.

The main objective of this recommendation is to establish the best strategies for obesity management in the first medical appointmet. For this, we formulated three "PICO questions" and we have answered based on the analysis of the available scientific evidence. Our main conclusions were: • In the first medical appointment of an adult patient with obesity, the motivational interview is more effective compared to the traditional approach for weight loss since it also allows to reinforce the patient's motivation and stimulate their participation in a behavioral change. • For the obesity diagnosis, the body mass index continues to be a useful and simple detection tool; however, it is imperative to extend the view of obesity and establish the risk of complications in the first appointment, for this both the Edmonton staging system and the ABCD method are useful tools in addition to the body mass index. • Aerobic physical activity is still recommended for its benefit in fat mass loss, mainly visceral, however, when combining anaerobic physical activity, the results are superior to the isolated aerobic strategy. Conclusions: the approach to obesity in the first consultation should be based on a motivational interview to improve adherence; the diagnosis of obesity should be made by calculating the body mass index with an aditional tool that allows predicting the risk of complications; physical activity should combine aerobic as well as anaerobic strategies to improve both anthropometric and metabolic parameters.

Supervisão acadêmica do Projeto Mais Médicos: reflexão sobre uma experiência na Bahia / Academic advisement within the Mais Médicos Project: reflections on an experience in Bahia / Supervisión académica del Proyecto Más Médicos: reflexión sobre una experiencia en Bahía

O texto traz uma reflexão sobre a experiência de supervisão acadêmica realizada no âmbito do Projeto Mais Médicos (PMM), em Salvador e Lauro de Freitas, no período de 2019 a 2021, buscando identificar o papel cumprido pelas atividades de supervisão, suas contribuições e seus limites vis-à-vis à competência definida nos documentos oficiais. Para tanto, foram analisados relatórios de supervisão e registros das reuniões de tutoria, os quais foram cotejados com a vivência do autor na função de tutor. Os resultados mostram que a supervisão cumpre o papel de apoiar o desenvolvimento de competências dos médicos atuantes no PMM, mas não alcança o objetivo normativamente definido de promover a integração ensino-serviço. Em suma, a supervisão tem um papel positivo, embora restrito, de fortalecimento da Atenção Básica.

This paper reflects on the experience of academic advisement carried out within the More Doctors Project (PMM) in Salvador and Lauro de Freitas, Brazil, from 2019 to 2021, seeking to identify the role played by advisement activities, their contributions and limitations in relation to the official documents. For this purpose, advisor reports and records of tutoring meetings were analyzed, which were then compared with the author's experience as a tutor. Results show that advising fulfils the role of helping to develop the competences of physicians working in the PMM, but does not reach the normatively defined objective of promoting education-service integration. In short, advisor work has a positive, albeit restricted, role in strengthening Primary Care.

Este texto reflexiona sobre la experiencia de supervisión académica realizada en el ámbito del Proyecto Más Médicos (PMM), en Salvador y Lauro de Freitas (Brasil), de 2019 a 2021, con el fin de identificar el papel de las actividades de supervisión, sus aportes y límites frente a la competencia definida en los documentos oficiales. Para ello, se analizaron informes de supervisión y actas de reuniones de tutoría, los cuales se compararon con la experiencia del autor en el rol de tutor. Los resultados muestran que la supervisión cumple el papel de apoyar el desarrollo de competencias de los médicos que actúan en el PMM, pero no alcanza el objetivo definido normativamente de promover la integración enseñanza-servicio. En definitiva, la supervisión tiene un papel positivo, aunque restringido, en el fortalecimiento de la Atención Primaria.

Percepção de gestores e profissionais sobre o projeto "Mais Médicos para o Brasil" no cenário de pandemia do Coronavírus em um município do Sul da Bahia / The "More Doctors for Brazil" project: perception of managers and professionals during the Coronavírus pandemic in Southern Bahia / Percepción de gestores y profesionales sobre el proyecto "Más Médicos para Brasil" en la pandemia del Coronavirus en un municipio del Sur de Bahía

Este artigo analisa a percepção dos gestores e dos profissionais envolvidos no Projeto Mais Médicos sobre os desafios do provimento e da reorganização do processo de trabalho médico frente ao cenário de pandemia do coronavírus no município de Ilhéus (BA), em 2020. A produção de dados foi feita por meio de revisão documental e entrevistas aos gestores de saúde do município e a quatro profissionais médicos contratados pelo programa. A análise dos resultados contemplou a percepção dos gestores acerca do provimento médico no município bem como as ações de monitoramento, supervisão e avaliação do desempenho dos profissionais realizadas pela coordenação da Atenção Básica. A análise do processo de trabalho médico, por sua vez, tratou de identificar se houve mudanças nos objetos, meios de trabalho utilizados pelos profissionais e formas de organização do trabalho, antes e durante a eclosão da pandemia da covid-19. As conclusões apontam que, apesar de o número de vagas ser insuficiente para atender as necessidades do município em relação à cobertura da Atenção Básica, o provimento e fixação de médicos por meio do Projeto Mais Médicos para o Brasil ampliou o acesso da população aos serviços e, consequentemente, ao atendimento de necessidades decorrentes da alta prevalência de doenças crônicas, desnutrição, violência doméstica e, mais recentemente, da pandemia da covid-19.

This paper analyzes the challenges perceived by managers and professionals involved in the Mais Médicos Project regarding the provision and reorganization of the medical work process during the coronavirus pandemic in the municipality of Ilhéus, Bahia, Brazil, in 2020. Data was collected by means of a document review and interviews with the municipality's health managers and four medical professionals hired by the project. Result analysis included the managers' perception regarding medical supply in the municipality, as well as the monitoring, supervision, and evaluation of professional performance carried out by the Primary Care coordination. Analysis of the medical work process, in turn, tried to identify whether the objects, means of work used by professionals, and ways of work organization changed before and during the outbreak of the COVID-19 pandemic. The findings indicate that, despite the insufficient number of vacancies to meet the needs of the municipality in terms of Primary Care coverage, the provision and retention of physicians by the More Doctors for Brazil project expanded the population's access to services and, consequently, to meeting needs arising from the high prevalence of chronic diseases, malnutrition, domestic violence, and, more recently, the COVID-19 pandemic.

El objetivo de este trabajo es analizar la percepción de los gestores y profesionales involucrados en el Proyecto Más Médicos sobre los desafíos de la prestación y reorganización del proceso de trabajo médico frente al escenario de la pandemia del coronavirus en el municipio de Ilhéus, en Bahía (Brasil), en 2020. Los datos se obtuvieron mediante la revisión documental y entrevistas con los gestores de salud del municipio y cuatro profesionales médicos contratados por el programa. El análisis de los resultados incluyó la percepción de los gestores sobre la prestación médica en el municipio, así como las acciones de seguimiento, supervisión y evaluación del desempeño de los profesionales realizadas por la coordinación de atención primaria. El análisis del proceso de trabajo médico, a su vez, trató de identificar si hubo cambios en los objetos, medios de trabajo utilizados por los profesionales y formas de organización del trabajo antes y durante el estallido de la pandemia del Covid-19. Las conclusiones indican que, a pesar de que el número de plazas es insuficiente para satisfacer las necesidades del municipio en cuanto a la cobertura de la Atención Primaria, la provisión y fijación de médicos a través del Proyecto Más Médicos para Brasil amplió el acceso de la población a los servicios y, en consecuencia, la satisfacción de las necesidades derivadas de la alta prevalencia de enfermedades crónicas, la desnutrición, la violencia doméstica y, más recientemente, la pandemia del Covid-19.