RESUMEN
Objective: By comparing the acute toxicity of different extracts from Coreopsis tinctoria on mice, combined with the HPLC fingerprint and multiple linear regression to analyze the element which plays the most important role in causing the death of mice, and to provide the safety data for improving the extraction technology. Methods: To measure the maximum dose and maximal tolerance dose (MTD) of all the extracts, to measure the median lethal dose (LD50) by Bliss, and to record the death and weight changes; To measure the fingerprints of the extracts by HPLC, and to determine the element which mostly induced the death of mice by analyzing the absorption peak of the extracts by HPLC fingerprint with multiple linear regression. Results: The extracts include aqueous extract by spray drying (SD), aqueous extract by vacuum drying (VD) process, ethanol extract (ETE), ethyl acetate extracted component (AC), and the ethyl acetate extracted residuum (AR). Among those extracts, the maximum dose of SD and AR is 36 g/kg, the MTD of the VD is 26 g/kg, the LD50 (95% confidence limits) of ETE and AC are 19.565 (17.558-21.734) g/kg and 16.414 (13.987-34.725) g/kg, respectively; Under the high dose situation, 3,5-dicaffeoylquinic acid properly is the component which mostly contributes to the death of mice. Conclusion: Under the high dose situation, the ETE and AC will lead the death, and 3,5-dicaffeoylquinic acid properly is the component which mostly contributes to the death of mice.
RESUMEN
Objective: To determine the maximal tolerance of Xunengda Tablets on mice and explore curative effects of its antithrombotic effect and provide the theoretical basis for selecting drugs. Methods:Mice were given maximal dose, 26g?kg -1 of body weight, XND Ig once; Divided 53 mice into five groups, they were administered XND tab.(3g?kg -1 ), RSC(1.25g?kg -1 ), CDDP(1.25g?kg -1 ), XSTL(2.5g?kg -1 ), and NS once daily for 7 days, repectively; 1h after treatment the seventh day, the animals of groups iv. were given collagen adr by producing whole organism thrombus, observing the mortality, They were determined coagulation time with the blood capillaries and the number of writhings per mouse was recorded in order to determine pain. Results:Maximal tolerance (Ig) is 26g?kg -1 , 886.7 times the dose of clinical application apart from XSTL, three kinds of drug showed the avidence of antithrombotic effect ( P