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OBJECTIVE: Compared with the compound captopril tablets (compound Cap), to study the pharmacokinetics characteristics and bioavailability in twelve wista rats after oral administration of Cap pulsed pellets and compound CAP tablets by HPLC. METHODS: To prepare captopril pulsed pellets (Cap pulsed pellets). The pharmacokinetic parameters were computed by software program DAS2.1. RESULTS: In vivo the lag time for 4.75 h, compared with ordinary tablet, it has an obviously lag time, tmax was 9.67 h and the relative bioavailability was(117.29 ± 46.87)%. CONCLUSION: The release of CAP from CAP pulsed sustained-release pellets was shown to be sustained-release after an conspicuous lag time in vitro and in vivo. So the drug can be taken by the patient before sleeping and can achieve the purpose in the morning. Copyright 2012 by the Chinese Pharmaceutical Association.
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AIM:To establish the quality standard of the composition of Yuquan Micropill(Radix Rehmanniae,Fructus Schisandrae Chinensis,Radix Puerariae lobatae,etc.). METHODS: The composition of Yuquan Micropill were identified by TLC and the content was determined by HPLC.Puerarin could be separated through Diamonsil~(TM) C_(18) column(5 ?m,250 mm?4.6 mm) with methanol-water(1.2∶3 by volume) as a mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was set at 250 nm and the column temperature was maintained at 40 ℃.?-Schizandrin could be separated through Kromasil KR100-3.5 C_(18) column(100 mm?4.6 mm) with methanol-water(3.5∶1 by volume) as a mobile phase.The flow rate was 0.6 mL/min and the detection wavelength was set at 254 nm. RESULTS: The spots in the TLC were clear and could be well separated,the blank and adjuvant contrast could not be interfered.HPLC showed a good peak form,good resolution and could not be interfered by the foreign matter.The average recovery of puerarin was 99.24%,RSD=0.84%,RSD of precision was 0.95%,RSD of reproducibility was 0.86%,RSD of stability was 0.44%.The average recovery of ?-schizandrin was 99.74%,RSD=0.78%,RSD of precision was 1.89%,RSD of reproducibility was 1.76%,RSD of stability was 1.82%. CONCLUSION: The method is simple and has good resolution,strong specificity and good reproducibility,It can be used for the quality control of the composition of Yuquan Micropill.
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AIM: To prepare Ganmaoqing micro-pills (Rhizomaer Radix Baphicacanthis Cusiae. Folium Isatidis, etc). METHODS: Extruded-spheronizing granulator and fluidized coating-machine were applied for Ganmaoqing micro-pills' production. RESULTS: According to the method mentioned above, the content of Ganmaoqing micro-pills wasreached 96.27% paracetauol in labelled indicator. CONCLUSION: The method is feasible and convenient, the result is extract with stable quality.
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AIM: To establish a HPLC for determination of puerarin and baicalin in Gegen Qinlian Micropill. METHODS: A Kromasil C 18 Column was used. The mobile phase was methanol-0.2% phosphoric acid. Puerarin and baicalin were determined by dual wavelength, ? 1=250nm. ? 2=315nm. RESULTS: The linear range of puerarin was within 32.0 ng—288.0 ng,r=0.9998 and the sample recovery was 100.17 % (RSD=1.00%(n=9)). The linear range of baicalin was within 52.6 ng～447.1 ng, r=0.9999 and the sample recovery was 100.06 % (RSD=1.06% (n=9)). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control of Gegen Qinlian Micropill.