RESUMEN
Microsomal epoxide hydrolase (mEH) is a crucial biotransformation enzyme that has capability to metabolize a large number of structurally divergent, highly reactive epoxides, and numerous environmentally exposed carcinogens. It catalyzes the conversion of xenobiotic epoxide compounds into more polar diol metabolites and may play important part of the enzymatic defense against adverse effects of foreign compounds. Most commonly, two functional polymorphisms affecting mEH enzyme activity have been identified: One in exon 3 and other in exon 4 of the mEH gene, which results in His113Tyr and Arg139His amino acid substitutions, respectively. Recent reports have shown that polymorphisms in mEH gene loci may an important risk factor for susceptibility of prostate cancers (PCs), worldwide, but inconsistent finding were also be illustrated. To the best of our knowledge, globally, there is no any systematic review has been published related to mEH gene polymorphisms and PC risk. Thus, in the current review, we have discussed the association between mEH gene polymorphisms, gene–environmental interaction, and PC risk.
RESUMEN
Objective: This study intended to explore the relationships of the polymorphism of phase II drug-metabolizing enzyme, microsomal epoxide hydrolase 1 (EPHX 1) with the habits of smoking and alcohol drinking, and their interactions on risk of deve-loping primary hepatocellular carcinoma (HCC). Methods: The genotypes at codon 113 of exon 3 of gene EPHX 1 were analyzed in 105 patients with HCC and 151 healthy controls in Guangxi Province by using polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP). The habits of smoking and alcohol drinking of all the subjects were investigated. Results: The frequencies of Tyr/Tyr, Tyr/His and His/His genotypes at codon 113 of exon 3 of gene EPHX 1 were 27.62%, 21.90%, and 50.48% for HCC patients and 21.19%, 34.44%, and 44.37% for healthy controls, respectively. There was no significant difference (P > 0.05). Smoking increased the risk of developing HCC for patients with His/His genotype. The OR value was 2.99 (95% CI: 1. 29-6.91). For the HBV-positive patients, polymorphism of EPHX 1 and the habits of smoking and alcohol drinking had synergistic effects on the development of HCC. Conclusion: The interaction between the environmental factors such as smoking and alcohol drinking and polymorphism of EPHX 1 gene may increase the risk of developing HCC.
RESUMEN
Objective This study intended to explore the relationship of the polymorphisms of phase Ⅱ metabolic genes (GSTM1 and EPHX1), smoking, alcohol drinking and their interactions on risk of hepatocellular carcinoma(HCC). Methods Using multiplex PCR and PCR-RFLP, the genotypes of GSTM1 and EPHX1 were analyzed in 105 patients with HCC and 151 health controls in Guangxi. The state of smoking and alcohol drinking were investigated. Results The frequency of the GSTM1 null genotype in cases was 64.76% and 50.99% in controls, which was significantly different(P