Further characterization of six miniSTR loci in the Han population from China / 解放军医学杂志

Objective To describe the characteristics of two miniplex sets: NC01 (D10S1248, D14S1434 and D22S1045), which was recommended by EDNAP/ENFSI, and a new miniplex one (D2S2944, D18S872 and D19S591). Methods DNA was extracted using the Chelex-100 extraction method. The products were genotyped by ABI PRISM® 310 Genetic Analyzer and the results were analyzed with GeneScan 3.7 and GenoTyper 3.7 software. Results All loci meet Hardy-Weinberg equilibrium. The combined power of discrimination for the six loci in Chinese population was 0.9999 and the cumulative probability of exclusion was 0.9793. We also compared the sequencing data of NC01 with other different ethic groups. Conclusion Two miniplex sets were constructed. These miniSTR makers have different characteristics in different ethic groups.

Polymorphism study of six miniSTR loci in Han population from Northeast China / 中国组织工程研究

OBJECTIVE:Allele frequencies and forensic parameters for six miniSTR loci(D10S1248,D14S1434,D22S1045,D4S2364,D2S441 and D1S1677)were analyzed in a population of 173 unrelated Chinese Han population individuals from Northeast China.METHODS:The six miniSTR loci were preformed in two multiplex fluorescent PCR systems.ABI 310 Genetic Analyzer was utilized in capillary electrophoresis and the lengths of allele fragments were analyzed.Genetic data collection and analysis software were used for data collection and genotyping.Statistical analysis was performed to the data.RESULTS:The six miniSTR loci showed a moderate degree of polymorphism in Han population from Northeast China.The observed allele sizes were from 67 bp to 115 bp,and the observed heterozygosity ranged from 0.728 to 0.827.The combined power of discrimination and the combined power of exclusion for the six miniSTR loci in Northeast China were 0.999 99,and 0.993 31,respectively.CONCLUSION:The six miniSTR showed a moderate genetic polymorphism in Han population from northeast of China.Due to their small size of PCR amplicon,the six miniSTR could be useful supplements to the CODIS STRs,and they would be useful in population genetics and forensic analysis.

Application of MiniSTR in the detection of fetal DNA in maternal plasma / 中国输血杂志

Objective To develop an efficient method for detecting the short tandem repeat(STR) of fetal DNA in maternal plasma by miniSTR technique.Methods A total of 9 blood samples form pregnant women from 11 to 27 weeks of gestation were collected.Each isolated total plasma DNA was amplified in single multiplex using the ABI MiniFilerTM kit,which could simultaneously genotype the 9 miniSTR loci,including D13S317,D7S820,D2S1338,D21S11,D16S539,D18S51,CSFIPO,FGA and Amelogenin,and the PCR products were detected by using ABI PrismTM 3100 DNA Sequencer.The allelic designation of each STR locus was accomplished using the GeneMapper ID 3.2 software.Results Father-origin fetal STR allele was detected in all the 9 plasma DNA samples.An average of 3.1 fetal STR alleles of the 8 autosomal STR loci was observed in each of the 9 plasma DNA samples.As for the Amelogenin locus,Amelogenin Y allele was detected in 5 plasma DNA samples from pregnancies with male fetus,and allelic peak height values were all over 50 RFU,according to ABI Mini FilerTM PCR conditions,and the ratio of Amelogenin Y allele peak height value to Amelogenin X allele peak height value was 8.45%.However,no Amelogenin Y allele was detected in other 4 plasma DNA samples from pregnant women with female fetus.Conclusion The miniSTR technique is suitable for STR genotyping using fetal DNA in maternal plasma,and it suggests a broad application in noninvasive molecular prenatal diagnosis.

Evaluation of Nine Non-CODIS MiniSTR Loci to aid Analysis of Degraded DNA / 대한법의학회지

For highly degraded DNA samples of forensic casework, new miniSTR PCR systems have been developed to supplement the current CODIS STRs. In the present study, we established the three miniplexes for nine miniSTRs (NC01 : D10S1248, D14S1434 and D22S1045; NC02 : D1S1677, D2S441 and D4S2364; and NC03 : D3S3053, D6S474 and D20S482) which had been previously suggested by Butler group (NIST, Gaitherburg, MD, USA). To evaluate the usefulness of the nine miniSTRs in analysis of degraded DNA, the sensitivity and efficacy of the three miniplexes were determined and then compared with those of the BigMini STR system which consists of six CODIS miniSTRs (TH01, CSF1PO, FGA, TPOX, D7S820, and D21S11). The three miniplexes gave better results in both the sensitivity test and efficiency test in comparison with BigMini. In the sensitivity test using serially diluted standard DNA, most loci in the three miniplexes showed reliable results for samples containing 50 pg of DNA and some even showed good sensitivity for samples containing 30 pg of DNA. Additionally, the three miniplexes generated useful profiles for both enzymatically degraded DNA and 50-year old skeletal remain samples. Among the nine miniSTRs, D4S2364, D3S3053, D14S1434, and D1S1677 produced the most successful DNA profiles for old skeletal remains. These results suggest that new miniSTRs could be useful supplements to the 13 CODIS STRs for forensic analysis of degraded DNA.

The application of MiniFiler~(TM) kit in LCN-STR genotyping / 中国法医学杂志

Objective To evaluate the application value of MiniFilerTM kit in LCN-STR genotyping in forensic science.Methods Compared the testing results of MiniFilerTM and IdentifilerTM kits in 49 general quantity biological samples and 39 LCN-biological samples,including blood stains,sperm stains,bones,and so on.Meanwhile the sensitivity of these two kits were also compared.Results Full concordance between IdentifilerTM and MiniFilerTM kits was observed in all of 49 general quantity biological samples.For 39 LCN-biological samples,only 22 samples could be genotyped in partial loci and 17 samples negative with the IdentifilerTM kit,while 30 samples could be genotyped in all loci,5 samples in partial loci and 4 samples negative with the MiniFilerTM kit.Therefore,there was a higher success rate for LCN-biological samples typing with MiniFilerTM kit than with IdentifilerTM kit.In addition,the sensitivity of the MiniFilerTMkit was also a little higer than the IdentifilerTMkit.Conclusion The results demonstrated that the MiniFilerTM kit can markedly improve the typing success rate of LCN-biological samples,and is suitable for analyzing difficult biological samples in forensic practice.