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Objective To investigate the effects of modified Yupingfeng Powder medicated serum on the inflammatory response of rat lung epithelial type Ⅱ cells RLE-6TN;To explore the mechanism based on nuclear factor(NF)-κB signaling pathway.Methods The inflammatory reaction of RLE-6TN cells was induced by lipopolysaccharide,and the cells were divided into control group,model group,dexamethasone medicated serum group,and 5%,10%and 20%TCM serum group,and the cell model was intervened with different serum.After 24 hours,the content of lactate dehydrogenase(LDH)in cell supernatant was detected by kit,and the content of reactive oxygen species(ROS)in cells were detected by fluorescence staining,Hoechst/PI double staining was used to observe cell apoptosis,and ELISA was used to detect tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-1β contents in cell supernatant,RT-qPCR was used to detect Toll like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),and NF-κB inhibitory protein α(IκBα)mRNA expression,Western blot was used to detect protein expressions of TLR4,MyD88,NF-κBp65 and IκBα.Results Compared with the control group,the contents of LDH,TNF-α,IL-6,IL-1β in RLE-6TN cell supernatant and ROS content in cells of model group significantly increased(P<0.01),the cell apoptosis rate significantly increased(P<0.01),the expressions of TLR4 and MyD88 mRNA and protein significantly increased(P<0.01),the expression of IκBα mRNA significantly decreased(P<0.01),and the ratio of p-NF-κBp65/NF-κBp65 and p-IκBα/IκBα significantly increased(P<0.01).Compared with the model group,the content of LDH,TNF-α,IL-6,IL-1 in RLE-6TN cell supernatant and ROS content in cells significant decreased in the 20%TCM serum group and dexamethasone medicated serum group(P<0.01),the cell apoptosis rate significantly decreased(P<0.01),the expressions of TLR4 and MyD88 mRNA and protein significantly decreased(P<0.05,P<0.01),the expression of IκBα mRNA significantly increased(P<0.01),and the ratio of p-NF-κBp65/NF-κBp65 and p-IκBα/IκBα significantly decreased(P<0.05,P<0.01).Conclusion Modified Yupingfeng Powder medicated serum can reduce RLE-6TN cells inflammatory reaction,and its mechanism may be related to the regulation of NF-κB signaling pathway.
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[Objective]To explore the clinical efficacy of modified Yupingfeng Powder combined with western medicine in the treatment of pregnant women with mild corona virus disease 2019(COVID-19).[Methods]The pregnant women with mild COVID-19 admitted to Shanghai Public Health Clinical Center from April 2022 to June 2022 were divided into treatment group and control group randomly.The control group(52 cases)was treated with western medicine such as Vitamin C,and the treatment group(48 cases)was treated with modified Yupingfeng Powder on the basis of control group.A comparison was made between the two groups in terms of each symptoms scores,incidence of adverse pregnancy outcomes and total effective rate.Besides,the levels of laboratory indicators involving lymphocyte,C reactive protein(CRP),interleukin-2 receptor(IL-2R)and interleukin-6(IL-6)were also be compared.The negative changes of virus nucleic acid and disease outcome were observed in the two groups.[Results]Compared with control group,after treatment cessation,the total symptom score in treatment group significantly decreased(P＜0.001),and the total effective rate significantly increased(P＜0.01).The levels of inflammatory markers including CRP and IL-6 decreased significantly in treatment group(P＜0.001,P＜0.01),while the count of lymphocyte increased significantly(P＜0.001).Besides,there was no difference in the incidence of adverse pregnancy outcomes between the two groups(P＞0.05).[Conclusion]Modified Yupingfeng Powder combined with western medicine can significantly relieve the clinical symptoms of pregnant women with mild COVID-19,while shortening nucleic acid negative conversion time and does not affect the pregnancy outcome,which has certain clinical value and is worthy of clinical application.
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OBJECTIVE:To observe clinical efficacy and safety of Modified yupingfeng powder decoction in the treatment of elderly patients with type 2 diabetes hyperhidrosis.METHODS:A total of 120 elderly patients with type 2 diabetes hyperhidrosis se-lected from outpatient of our hospital during Jan. 2015 to Jun. 2016 were divided into control group and observation group accord-ing to random number table,with 60 cases in each group. Control group was given routine chemical drug therapy of diabetes. Ob-servation group was additionally given Modified yupingfeng powder,decocted with water,300 mL each time,150 mL morning and night,1 h before meal,on the basis of control group.Treatment courses of 2 groups both were 1 month. Blood glucose index-es(FPG,2 hPG,HbA1c)and TCM symptom scores before and after treatment were compared between 2 groups. Clinical effica-cies and the occurrence of ADR were observed. RESULTS:Before treatment,there was no statistical significance in FPG,2 hPG, HbA1c or TCM symptom scores between 2 groups(P>0.05). Compared to before treatment,FPG,2 hPG,HbA1c and TCM symp-tom scores of 2 groups were decreased significantly after treatment;the levels of FPG,HbA1c and TCM symptom score in observa-tion group were significantly lower than control group,with statistical significance(P<0.05). But there was no statistical signifi-cance in 2 hPG between 2 groups(P>0.05). Total response rate of observation group(90.00%)was significantly higher than that of control group(70.00%),with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Modified yupingfeng powder decoction can effectively improve the clinical symptoms,and shows good therapeutic efficacy and safety for elderly patients with type 2 diabetes hyperhidrosis.
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OBJECTIVE:To observe clinical efficacy and safety of Modified yupingfeng powder decoction in the treatment of elderly patients with type 2 diabetes hyperhidrosis.METHODS:A total of 120 elderly patients with type 2 diabetes hyperhidrosis se-lected from outpatient of our hospital during Jan. 2015 to Jun. 2016 were divided into control group and observation group accord-ing to random number table,with 60 cases in each group. Control group was given routine chemical drug therapy of diabetes. Ob-servation group was additionally given Modified yupingfeng powder,decocted with water,300 mL each time,150 mL morning and night,1 h before meal,on the basis of control group.Treatment courses of 2 groups both were 1 month. Blood glucose index-es(FPG,2 hPG,HbA1c)and TCM symptom scores before and after treatment were compared between 2 groups. Clinical effica-cies and the occurrence of ADR were observed. RESULTS:Before treatment,there was no statistical significance in FPG,2 hPG, HbA1c or TCM symptom scores between 2 groups(P>0.05). Compared to before treatment,FPG,2 hPG,HbA1c and TCM symp-tom scores of 2 groups were decreased significantly after treatment;the levels of FPG,HbA1c and TCM symptom score in observa-tion group were significantly lower than control group,with statistical significance(P<0.05). But there was no statistical signifi-cance in 2 hPG between 2 groups(P>0.05). Total response rate of observation group(90.00%)was significantly higher than that of control group(70.00%),with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Modified yupingfeng powder decoction can effectively improve the clinical symptoms,and shows good therapeutic efficacy and safety for elderly patients with type 2 diabetes hyperhidrosis.